Inoculation and expanding propagation method of fusarium verticillatum

A technology of Fusarium verticillium, injection and inoculation, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problem of ear rot that is not suitable for large-scale inoculation of Fusarium verticillium, and sticky flakes. In the case of problems such as uneven inoculation on the wall of the culture container, it can improve the propagation speed, save labor and time costs, and improve the uniformity.

Active Publication Date: 2021-04-06
YUAN LONGPING HIGH TECH AGRI CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following problems: (1) low efficiency; (2) low sporulation yield; (3) uneven inoculation; (4) a large number of culture dishes are required for propagation; Desporulation; (6) bacterial flakes are easy to stick on the culture container wall; (7) easy to pollute; the above problems make the traditional method of propagation of Fusarium pseudoverticillium unsuitable for large-scale inoculation of Fusarium pseudoverticillium ear rot

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  • Inoculation and expanding propagation method of fusarium verticillatum
  • Inoculation and expanding propagation method of fusarium verticillatum

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Embodiment 1

[0031] The present embodiment provides a method for inoculating and multiplying Fusarium pseudoverticillium, and the specific steps are as follows:

[0032] 1. PDA propagation of Fusarium pseudoverticilis: inoculate the selected target Fusarium pseudoverticillium strains on PDA medium, put each 90mm*90mm petri dish with 15g of PDA medium into a 0.9cm diameter dish The fungus cake was cultivated at 28°C for 7 days.

[0033] 2. Inoculate sorghum grains with Fusarium pseudoverticillium bacteria liquid: put 500g sorghum grains into a 20cm*40cm double-opening polypropylene edible mushroom bag, seal both sides with double collars with a diameter of 4cm, and sterilize in an autoclave for 1 hour Afterwards, take it out, and put the sorghum grains down to room temperature into an aseptic operating table; put the culture of Fusarium pseudovermitilis with a purity of 100% (including all solid medium in the pathogenic bacteria and the petri dish) that is obtained in step 1 into a squeezer...

Embodiment 2

[0037] This embodiment provides a method for inoculating and multiplying Fusarium pseudoverticillium. The difference from Example 1 is that a 500mL triangular flask is used instead of a double-opening polypropylene edible fungus bag. The specific steps are as follows:

[0038] 1. PDA propagation of Fusarium pseudoverticilis: inoculate the selected target Fusarium pseudoverticillium strains on PDA medium, put each 90mm*90mm petri dish with 15g of PDA medium into a 0.9cm diameter dish The fungus cake was cultivated at 28°C for 7 days.

[0039]2. Inoculate sorghum grains with Fusarium pseudoverticillium bacteria liquid: put 200g sorghum grains into a 500mL triangular flask, seal it with a sealing film, take it out after autoclaving for 1 hour, and put the sorghum grains into aseptic operation after cooling down to room temperature platform, put the 100% culture of Fusarium pseudoverticillium purity obtained in step 1 (comprising pathogenic bacteria and all solid medium in the pet...

experiment example 1

[0052] Measure respectively the spore amount and the purity of the Fusarium pseudoverticillium that the inoculation and propagation method of each embodiment and comparative example obtain, wherein embodiment 1 carries out the propagation test of 5 bacteria bags, and embodiment 2 carries out 5 triangular flask liquids The multiplication test of inoculation, comparative example 1 carries out the multiplication test of 200 dishes, and comparative example 2 carries out the multiplication test of 5 triangular flask solid inoculations, and concrete result is as follows, wherein the statistical result of sporulation amount and purity is as shown in table 1 Show.

[0053] The method of Example 1: from step 2 to the end of inoculation, the time required to inoculate a bag of sorghum grains is about 20-24 seconds; every 20 petri dishes are squeezed to obtain bacterial liquid, and continuous syringes can be used to inoculate 80 bags of sorghum grains ; The spore concentration of Fusariu...

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Abstract

The invention relates to the technical field of expanding propagation of plant pathogenic fungi, in particular to an inoculation and expanding propagation method of fusarium verticillatum. The inoculation and expanding propagation method of the fusarium verticillatum comprises the steps of solid culture medium expanding propagation and plant seed expanding propagation, and specifically comprises the following steps of preparing a bacterial solution from the fusarium verticillatum obtained by solid culture medium expanding propagation and an attached solid culture medium thereof, and injecting and inoculating the bacterial solution into a culture container filled with plant seeds for expanding propagation. The expanding propagation method significantly improves the spore production efficiency of the fusarium verticillatum, reduces the cost, has the advantages of simple operation, low pollution rate, uniform inoculation, no adhesion of inoculums to a propagation container, high spore production amount, uniform growth, convenient unified delivery and the like, and can be used for large-scale inoculation and expanding propagation of the fusarium verticillatum in practice.

Description

technical field [0001] The invention relates to the technical field of propagation of plant pathogenic fungi, in particular to a method for inoculating and propagating Fusarium pseudoverticillium. Background technique [0002] In recent years, changes in crop farming systems and changes in climate have made the agricultural field more and more demanding on the disease resistance of crops. Fusarium verticillioides is a common plant pathogenic fungus. Under natural environmental conditions, Fusarium pseudoverticillium can often cause corn ear rot, and the fumonisin (FB) produced by it will contaminate food and its products, and cause acute toxicity and potential carcinogenicity to some livestock . At present, the most effective and environmentally friendly method for corn ear rot is to cultivate and promote disease-resistant varieties, and to identify whether crops have resistance to Verticillium pseudoverticillium ear rot requires the production of sufficient quantities of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 王建海顾美玲杨乾阮祥经
Owner YUAN LONGPING HIGH TECH AGRI CO LTD
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