Preparation method of antibiotic-crosslinked specific demineralized extracellular matrix scaffold

A technology of antibiotics and extracellular matrix, applied in the field of bone infection treatment, can solve the problems of inability to prevent re-infection of "latent" bacteria, unfavorable for tissue repair and osteogenesis, and no longer have antibacterial properties, so as to achieve sufficient reference, prevention and treatment. Bone infection, good biocompatibility

Active Publication Date: 2021-04-09
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

First of all, due to the difference in surface free energy between inorganic and organic media, this modification is not simple, and the unstable coating will inevitably fall off with the passage of time and changes in the internal environment, resulting in a decrease in the biocompatibility of the material; Secondly, with the complete release of antibiotics or antimicrobial peptides, the material itself no longer has antibacterial properties and becomes the object of bacterial adhesion, and cannot prevent the reinfection of "latent" bacteria; finally, metal ions are cytotoxic, which is not conducive to tissue repair and osteogenesis

Method used

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  • Preparation method of antibiotic-crosslinked specific demineralized extracellular matrix scaffold
  • Preparation method of antibiotic-crosslinked specific demineralized extracellular matrix scaffold
  • Preparation method of antibiotic-crosslinked specific demineralized extracellular matrix scaffold

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Embodiment 1

[0076] The preparation method of the specific demineralized extracellular matrix scaffold (Van-SDECM) of cross-linked vancomycin, the specific steps are as follows:

[0077] (1) Fresh pork scapula is obtained with a hollow bone drill with a diameter of 6mm to obtain cancellous bone, and then cut into cylinders with a thickness of 3mm;

[0078] (2) Wash the cancellous bone with tap water for 1 hour, pack it in an embedding box, and then soak it in 0.6% (v / v) peracetic acid ultrapure water for 1; 1% (v / v) Triton-X100 After filtering and sterilizing with a 0.22 μm filter head, transfer the embedding cassette to a Triton-X100 solution flask, shake at 100 rpm, 4°C for 24 h; wash the embedding cassette with 500 ml of sterilized water, and keep stirring for 1 h, repeat twice; Then add the cancellous bone block into 1% (w / v) sodium dodecyl sulfate (SDS) aqueous solution, shake at 100 rpm, 4°C for 36 hours, and wash the embedding cassette again;

[0079] (3) Soak cancellous bone ECM i...

Embodiment 2

[0082]The preparation method of the specific demineralized extracellular matrix scaffold (Van-SDECM) of cross-linked vancomycin, the specific steps are as follows:

[0083] (1) Fresh pork scapula is obtained with a hollow bone drill with a diameter of 4mm to obtain cancellous bone, and then cut into cylinders with a thickness of 2mm;

[0084] (2) Wash the cancellous bone with tap water for 1 hour, pack it in an embedding box, and then soak it in 0.6% (v / v) peracetic acid ultrapure water for 1; 1% (v / v) Triton-X100 After filtering and sterilizing with a 0.22 μm filter head, transfer the embedding cassette to a Triton-X100 solution flask, shake at 100 rpm, 4°C for 12 h; wash the embedding cassette with 500 ml of sterilized water, and keep stirring for 1 h, repeat twice; Then add the cancellous bone block into 1% (w / v) sodium dodecyl sulfate (SDS) aqueous solution, shake at 100 rpm, 4°C for 12 hours, and wash the embedding cassette again;

[0085] (3) Soak cancellous bone ECM in...

Embodiment 3

[0088] The preparation method of the specific demineralized extracellular matrix scaffold (Van-SDECM) of cross-linked vancomycin, the specific steps are as follows:

[0089] (1) Fresh pork scapula is obtained with a hollow bone drill with a diameter of 8mm to obtain cancellous bone, and then cut into cylinders with a thickness of 4mm;

[0090] (2) Wash the cancellous bone with tap water for 1 hour, pack it in an embedding box, and then soak it in 0.6% (v / v) peracetic acid ultrapure water for 1; 1% (v / v) Triton-X100 After sterilizing with a 0.22 μm filter head, transfer the embedding cassette to a Triton-X100 solution flask, shake at 100 rpm, 4°C for 48 h; wash the embedding cassette with 500 ml of sterilized water, and keep stirring for 1 h, repeat twice; Then add the cancellous bone block into 1% (w / v) sodium dodecyl sulfate (SDS) aqueous solution, shake at 100 rpm, 4°C for 48 hours, and wash the embedding cassette again;

[0091] (3) Soak cancellous bone ECM in 10% EDTA dec...

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Abstract

The invention discloses a preparation method of an antibiotic-crosslinked specific demineralized extracellular matrix scaffold, and belongs to the technical field of bone infection treatment. The invention discloses the preparation method of the antibiotic-crosslinked specific demineralized extracellular matrix scaffold, which comprises the following steps of by using a specific demineralized and decellularized cancellous bone extracellular matrix scaffold (SDECM) as a substrate, carrying out compound drug loading on the substrate and the antibiotics in two forms of electrostatic adsorption and chemical crosslinking, releasing antibiotics in two modes of rapid pH response and accompanying material degradation in an acid environment, and adopting the antibiotics for resisting infection and promoting repair through SDECM, so that the pH-sensitive release drug in the early acidic environment of infection is rapidly sterilized, the drug is slowly released in the middle-late physiological environment for continuous sterilization, the treatment effect of permanent sterilization capability and prevention of infection recurrence is still achieved in the absence of infection, and a new thought and a new tool are provided for antibacterial and repair treatment of bone infection.

Description

technical field [0001] The invention relates to the technical field of bone infection treatment, in particular to a method for preparing a cross-linked antibiotic-specific demineralized extracellular matrix scaffold. Background technique [0002] Despite the continuous improvement of the medical level, bone infection is still a huge problem in clinical work, and nearly 40% of patients are faced with recurrence and persistent infection. In the pathological process of bone infection, bacteria multiply and form abscesses in the infected area, avoiding the immune system to kill and protect the living bacteria at their core. Factors and acidic metabolites secreted by bacteria in abscesses not only promote the differentiation of osteoclasts , reduce the activity of osteoblasts, and at the same time help bacteria adhere to collagen or cell membranes, promote the formation of biofilms, and cause bone infections to persist. Among the five key factors that lead to infections that are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/00A61L27/50A61L27/54
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/3608A61L27/365A61L27/00A61L27/50A61L27/54A61L2430/40A61L2430/02A61L2300/602A61L2300/412A61L2300/406A61L2300/41
Inventor 林贤丰顾辰辉方斌范顺武
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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