A strain of Paenibacillus marseille and its application in the control of plant nematode diseases
A technology of Paenibacillus and bacterial strains, which is applied to Paenibacillus marseille and its application in the prevention and treatment of plant nematode diseases, can solve the problems that the application of nematode activity has not been reported, and achieve low production cost, stable control effect, and good biological control effect of effect
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[0047] The preparation method of microbial bacterial agent provided by the present invention, concrete steps comprise:
[0048] (1) Preparation of solid LB medium;
[0049] (2) Preparation of liquid LB medium;
[0050] (3) Activation of the strain: pick a colony of strain HMQAU19034, inoculate it in 50 mL of liquid LB medium, culture it at 200 rpm and 30°C for 24 hours with constant temperature shaking, and make a seed liquid;
[0051] (4) Preparation of fermentation broth: Inoculate the seed solution in 50 mL of liquid LB medium at an inoculum size of 1 / 50, culture at a constant temperature of 200 rpm and 30° C. for 48 hours, and prepare the fermentation broth of strain HMQAU19034;
[0052] (5) Preparation of fermentation filtrate: the prepared fermentation liquid was centrifuged at 4° C., 8000 r / min for 15 min, and the supernatant was filtered with a 0.22 μm microporous membrane to prepare the fermentation filtrate of strain HMQAU19034.
[0053] The solid LB medium provide...
Embodiment 1
[0055] Example 1 Isolation, screening and identification of antagonistic bacterial strains
[0056] (1) Separation and screening
[0057] In September 2019, American ginseng roots with root knots of root-knot nematodes were collected in Xiaoyuan Village, Wandi Town, Laiyang, Shandong Province. About 1 gram of American ginseng roots with root knots were sterilized on the surface, then cut into pieces with sterilized scissors, and added Sterilized water 1mL, grind with a sterilized mortar and pestle, separate the bacterial strains from the grinding juice by the dilution plate method, the medium is a solid LB medium, after the colonies grow, pick a single bacterial colony on the LB solid The plate was streaked and purified and saved.
[0058] (2) Morphological identification
[0059] Transfer the strains on the slant to the solid LB medium plate, carry out the three-section line, culture in the incubator at 30°C for 48 hours, observe the colony shape after growing into obvious ...
Embodiment 2
[0074] Example 2 Toxicity of bacterial strain HMQAU19034 to second instar larvae of M. incognita
[0075] (1) Acquisition of second instar larvae of M. incognita
[0076] Take out the root system of cucumber or water spinach containing a large number of root-knot nematode oocysts, rinse gently with water, carefully remove the oocysts, put them in 0.5% sodium hypochlorite for disinfection for 2 minutes, then rinse them with sterile water for 3 times, put the oocysts on the In a petri dish with a small amount of sterile water, cultivate in a 25°C incubator, and collect newly hatched second-instar larvae after 24 hours for subsequent use. In the present invention, second-instar larvae are called J2 larvae for short.
[0077] (2) Preparation of working solution of strain HMQAU19034 fermentation broth and fermentation filtrate
[0078] Take the fermentation broth of strain HMQAU19034, and use sterile water to adjust the cell concentration to 1.0×10 7 cfu m L -1 , which is the wo...
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