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A bacterial strain capable of removing cadmium ions and its application

A cadmium ion and reaction technology, applied in the field of bioengineering, can solve the problems of poor adsorption capacity of cadmium ions and long adsorption cycle, etc., and achieve the effect of wide application prospects and significant cadmium ion removal effect

Active Publication Date: 2022-05-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problems that the strains in the prior art have poor adsorption capacity for cadmium ions in the waste liquid and the adsorption cycle is long

Method used

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  • A bacterial strain capable of removing cadmium ions and its application
  • A bacterial strain capable of removing cadmium ions and its application
  • A bacterial strain capable of removing cadmium ions and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Isolation, screening and identification of Exiguobacterium acetylicum LBBE-U1 strain

[0055] 1. Isolation of strains

[0056] Specific steps are as follows:

[0057] (1) Obtain sludge samples from the biochemically treated anaerobic tank of Lucun Sewage Treatment Plant Phase III in Wuxi City, Jiangsu Province, put them in 50mL centrifuge tubes and store them in a refrigerator at 4°C;

[0058] (2) After removing impurities from the sample, draw 10 mL into a 250 mL shaker flask filled with 90 mL of sterile water, and vibrate on a shaker at 30°C and 220 r / min for 1 hour to obtain a sample suspension;

[0059] (3) Let the sample suspension stand for 5 minutes, take the supernatant for gradient dilution, and the dilution factor is 10 -1 、10 -2 、10 -3 ;

[0060] (4) Take 100 μL of each gradient dilution solution and spread them on the LB plates added with cadmium ions respectively, two parallels for each gradient, and place them in a constant temperature incu...

Embodiment 2

[0075] Example 2: Application of Exiguobacterium acetylicum LBBE-U1 strain in removing cadmium ions in waste liquid

[0076] Specific steps are as follows:

[0077] 1. Preparation of Exiguobacterium acetylicum LBBE-U1 bacterial suspension

[0078] (1) Pick a single colony of Exiguobacterium acetylicum LBBE-U1 and inoculate it into a 50mL centrifuge tube added with 10mL LB liquid medium, and culture it for 10-12h to obtain a seed solution;

[0079] (2) measure the OD of the seed liquid that step (1) obtains 600 value, and adjust the concentration of the seed solution to OD by saline 600 The value was 4, and the bacterial suspension was obtained.

[0080] 2. The acetyl microbacterium Exiguobacterium acetylicum LBBE-U1 bacterial suspension obtained in step 1 was prepared according to the final concentration OD 600 The additive amount with a value of 0.5 was added to 20mL simulated wastewater liquid containing 10mg / L and 5mg / L cadmium ions respectively. Among them, the composi...

Embodiment 3

[0082] Example 3: Properties of Exiguobacterium acetylicum LBBE-U1 strain

[0083] The Exiguobacterium acetylicum LBBE-U1 strain of the present invention can adapt to the environment of industrial waste water, and the lethality of the strain in the waste water environment is measured by taking simulated waste water samples as an example.

[0084] Specific steps are as follows:

[0085] (1) On the basis of the Exiguobacterium acetylicum LBBE-U1 bacterial suspension obtained in step 1 of Example 2, the bacterial suspension is adjusted to OD by physiological saline 600 The value is 0.5.

[0086] (2) the bacterial suspension that step (1) obtains is diluted to 10 -6 、10 -7 、10 -8 Three gradients, two parallels for each gradient, pipette 100 μL to coat LB plates, the number of colonies is 7.52×10 9 cfu / mL.

[0087] (3) the reaction solution after reacting 24h, 48h in the simulated wastewater sample in embodiment 2 is diluted to 10 respectively by gradient -6 、10 -7 、10 -8 ...

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Abstract

The invention discloses a bacterial strain capable of removing cadmium ions and an application thereof, belonging to the technical field of bioengineering. The present invention takes samples from nature to obtain heavy metal-tolerant bacterial strains, and uses 24 deep-well plate screening methods to carry out heavy metal adsorption experiments on them to obtain bacterial strains that can efficiently remove heavy metal ions and cadmium ions. After identification, the bacterial strains are Exiguobacterium acetylicum , preserved in the Guangdong Microbial Culture Collection Center, with the preservation number GDMCC No: 61321; the bacterial strain provided by the present invention is used to adsorb cadmium ions in wastewater, and the removal rate is the highest, up to 73.44%, when adsorbed for 48 hours.

Description

technical field [0001] The invention relates to a bacterial strain capable of removing cadmium ions and an application thereof, belonging to the technical field of bioengineering. Background technique [0002] With the rapid development of industrialization in my country, heavy metal pollution in natural water bodies and soil environments is becoming more and more serious. Heavy metal pollution mainly includes representative heavy metal ions such as copper, cadmium, lead, mercury, and hexavalent chromium, which usually come from electroplating, plastic manufacturing, mining and metallurgical process, etc. Heavy metal pollution in my country has a large amount, a wide range, and is difficult to deal with. It is always endangering the sustainable development of the ecology and posing a major threat to people's health. Therefore, it is urgent to effectively control heavy metal pollution in the environment. [0003] At present, the commonly used methods for removing heavy metals...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20
CPCC12N1/20C02F3/34C02F2101/20
Inventor 张娟房峻赵丹丹陈坚堵国成
Owner JIANGNAN UNIV