Sericin hydrogel as well as preparation method and application thereof

Abstract

The invention discloses sericin hydrogel and a preparation method thereof. The sericin hydrogel is prepared by reaction of a sericin aqueous solution and a cross-linking agent aqueous solution. The preparation method comprises the following steps: cleaning, drying and crushing silkworm cocoons without silk fibroin to obtain a silkworm cocoon powder, adding water, performing reaction at 100-180 DEG C for 0.5-10 hours, performing centrifuging to remove insoluble substances, and collecting a clarified solution to obtain a sericin aqueous solution with the concentration of 2-18%; and preparing a cross-linking agent aqueous solution, filtering and degerming by a filter membrane, adding sterilized material into the sericin aqueous solution, performing uniform mixing, and performing oscillating in a shaking table for 20-120 minutes. The sericin hydrogel has excellent mechanical property, porous microstructure, deformation memory property and sterility, can be used as a carrier of a drug or a growth factor, and is freeze-dried to obtain a sericin biological scaffold, and the scaffold can be applied to repair of tissue damage and treatment of diseases. The preparation method is simple, the gelling process is controllable, and the gelling time can be regulated and controlled by regulating the concentration of sericin.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a sericin hydrogel and a preparation method and application thereof. Background technique [0002] Sericin is a natural viscous protein secreted by the silkworm, which consists of polypeptides with a molecular weight of 24-400kDa and contains 18 kinds of amino acids. In recent years, people have found that sericin is not only non-immunogenic, but also has many excellent properties, such as degradable, anti-bacterial, anti-cancer, anti-oxidation, anti-ultraviolet, anti-coagulation, inhibition of tyrosinase activity, hydrophilic, moisturizing , and promote cell adhesion and proliferation, etc.; in addition, sericin has a large number of active groups that are convenient for people to modify or cross-link, such as amino, hydroxyl, carboxyl, etc. At present, sericin has become a new biomedical material favored by people. [0003] Currently, sericin has been used to p...

AI Technical Summary

Problems solved by technology

However, in terms of sterile sericin hydrogels, although sterile sericin hydrogels can be directly prepared by self-assembly, their mechanical properties are poor, lack of elasticity and are fragile.

On the other hand, long-term studies have believed that sericin obtained by high temperature and pressure has high water solubility, and it is difficult to prepare pure sericin hydrogel by direct crosslinking.

On the other hand, currently pure sericin hydrogels prepared based on sericin extracted from silkworm cocoons have low mechanical strength, and although the hydrogels prepared from sericin extracted from silkworm bodies have strong mechanical strength, however Obtaining sericin from silkworm body is time-consuming and laborious, and the operation is very difficult, so it is difficult to carry out large-scale production; at the same time, sericin protein in silkworm body and sericin isolated from silkworm cocoons also have great differences in structure and performance. Therefore, even if hydrogels with the same mechanical strength are prepared based on sericin obtained from silkworm bodies and sericin isolated from silkworm cocoons, the hydrogels prepared by the two have different physical and chemical properties and biological activities. There will still be large differences in terms of

In addition, there are few reports on whether silkworm feeding conditions and silk spinning environment affect the performance and extraction efficiency of sericin in silkworm cocoons.

Images