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Extraction method of centella asiatica polysaccharide extract, centella asiatica polysaccharide extract and application of same

An extraction method and technology of extracts, which can be used in drug combinations, pharmaceutical formulas, cosmetic preparations, etc., can solve problems such as difficulty in anti-inflammatory effects and high irritation of cosmetics

Inactive Publication Date: 2021-04-16
NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when the Centella asiatica extract currently on the market is added as a component of cosmetics, other irritating organic solvents usually need to be introduced, which makes the prepared cosmetics more irritating and difficult to play a safe anti-inflammatory effect.

Method used

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  • Extraction method of centella asiatica polysaccharide extract, centella asiatica polysaccharide extract and application of same
  • Extraction method of centella asiatica polysaccharide extract, centella asiatica polysaccharide extract and application of same
  • Extraction method of centella asiatica polysaccharide extract, centella asiatica polysaccharide extract and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Optimizing the Extraction Process of Centella Asiatica Polysaccharide Extract by Response Surface Methodology

[0063] 1 Experimental materials and instruments

[0064] 1.1 Experimental Instruments

[0065] Centella asiatica (produced in the Altay region of Xinjiang Uygur Autonomous Region) provided by Nanjing Institute of Comprehensive Utilization of Wild Plants, Jiangsu Province; NH-4 digital display constant temperature water bath (Santan Instrument Factory, Jintan City, Jiangsu Province); SHZ-DⅢ circulating vacuum pump ( Gongyi Yuhua Instrument Co., Ltd.); RE-52AA rotary evaporator (Shanghai Yarong Shenggong Instrument Factory); KF-1209 electronic balance (China Kaifeng Group Co., Ltd.); FA2004HM electronic balance (Changzhou Wantai Instrument Co., Ltd.); BCD-183A refrigerator (Hefei Rongshida Co., Ltd.); TG1650-WS desktop high-speed centrifuge (Hunan Xiangyi Laboratory Instrument Development Co., Ltd.); DS-1 tissue grinder (Beijing Huaxin Kechuang Technology Co., ...

Embodiment 2

[0126] Preparation method of anti-inflammatory cosmetics containing centella asiatica polysaccharide extract

[0127] Prepare components according to the following parts by mass:

[0128] Xanthan gum 0.25 parts, phenoxyethanol or ethylhexylglycerin 0.50 parts, propylene glycol 3.00 parts, glycerin 3.00 parts, ceteareth-6 olive oil 2.50 parts, cetyl palmitate or sorbitan Palmitate or sorbitan olive oil 3.50 parts, olive fruit unsaponifiable matter 0.50 parts, cetearyl alcohol 1.50 parts, dioctyl carbonate 3.00 parts, olive fruit oil 3.00 parts, octyl dodecanol 2.00 parts, 2.00 parts of dimethyl polysiloxane, 0.50 parts of cocoa seed butter, 0.50 parts of stearic acid, 0.50 parts of Centella asiatica extract provided in Example 1, 0.10 parts of tocopheryl acetate, 0.30 parts of essence and ionized water.

[0129]Take Xanthan Gum 0.25%, Phenoxyethanol / Ethylhexylglycerin 0.50%, Propylene Glycol 3.00%, Glycerin 3.00%, Ceteareth-6 Olivate 2.50%, Cetyl Palmitate / Sorbit Tanpalmitat...

Embodiment 3

[0132] Safety experiment of centella asiatica polysaccharide extract

[0133] 1 Experimental method

[0134] 1.1 Collection of experimental fish and their fertilized eggs

[0135] Sexually mature zebrafish were reared in separate tanks for sex and sex in a zebrafish culture unit. Water temperature: 26±2°C; pH 7.2; conductivity: 520μs / cm; light / dark cycle: 14h:10h. The day before the start of the exposure experiment, the zebrafish were paired with a male-to-female ratio of 1:2, and naturally mated and spawned under light stimulation.

[0136] 1.2 Exposure experiment

[0137] 1.2.1 Fish embryo safety experiment

[0138] (1) Select healthy zebrafish embryos developed to 0-3hpf (hours post-fertilization) and place them in a 6-well cell culture plate, 20 tails / well, and add 5 mL of Centella asiatica provided in Example 1 at a certain concentration to each well Solution of polysaccharide extract. The zebrafish culture water was used as the blank control group.

[0139] (2) At...

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Abstract

The invention belongs to the field of natural plant effective component separation and cosmetic additives, and particularly relates to an extraction method of a centella asiatica polysaccharide extract, the centella asiatica polysaccharide extract, and application of the same; the invention provides application of an asiatic pennywort herb polysaccharide extract in the anti-inflammatory aspect. The asiatic pennywort herb polysaccharide extract has good in-vivo anti-inflammatory activity. The invention also provides an extraction method of the centella asiatica polysaccharide extract, which comprises the following steps: extracting effective substances in centella asiatica by using deionized water and absolute ethyl alcohol to obtain the centella asiatica polysaccharide extract. By optimizing the extraction process, the extraction amount of the centella asiatica polysaccharide is effectively increased, and the extraction rate is as high as 0.603%.

Description

technical field [0001] The invention belongs to the field of natural plant active ingredient separation and cosmetic additives, and in particular relates to an extraction method of centella asiatica polysaccharide extract, centella asiatica polysaccharide extract and application thereof. Background technique [0002] Centella asiatica (L.) Urb., as a traditional Chinese medicine, is bitter in taste and cold in nature, and returns to the liver, spleen, and kidney meridians. The dry whole grass of Centella asiatica, a plant of the genus Centella, is also known as old crow bowl, horseshoe grass, copper money grass, money tooth grass, iron lamp lamp and big money grass. At present, it is mainly distributed in Anhui, Zhejiang, Hunan, Hubei, Jiangxi, Guangdong and other places in my country. The growth environment is mostly humid land or low grass. It likes humidity and tolerates shade, and can be harvested all year round. Centella asiatica was first recorded in "Shen Nong's Mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73C08B37/00A61Q19/00A61P29/00
Inventor 束成杰李卓航马世宏陈国庆汪海波李淑红单承莺聂韡
Owner NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP