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Traditional Chinese medicine effective components for treating eye diseases, and composition thereof

An eye disease and composition technology, applied in the field of medicine

Active Publication Date: 2021-04-16
SHANGHAI UNIV OF T C M +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the treatment of ocular diseases related to lymphangiogenesis, inflammation or bacterial infection with the composition of fencetin and fencetin in the present application.

Method used

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  • Traditional Chinese medicine effective components for treating eye diseases, and composition thereof
  • Traditional Chinese medicine effective components for treating eye diseases, and composition thereof
  • Traditional Chinese medicine effective components for treating eye diseases, and composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The extracts and Qinbing eyedrops used in the following examples are from Longhua Hospital, and teretin is purchased from Chengdu Desite Biotechnology Co., Ltd. Borneo was purchased from Aladdin Reagent (Shanghai) Co., Ltd., D-borneol was purchased from Shanghai Merrill Chemical Technology Co., Ltd., and macrophage Raw264.7 (ATCC TIB-71) cells were purchased from the Cell Bank of the Chinese Academy of Sciences. Purchased from the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine (Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.). RAW264.7 cell culture medium is DMEM medium (Thermo Fisher Biochemical Products (Beijing) Co., Ltd.) supplemented with 10% fetal bovine serum (FBS) (Serana Europe) and 1% penicillin-streptomycin double antibody (Life Technologies).

[0037] Preparation of Frucetin: 5 mg Frucetine was dissolved in 60 μl DMSO to make 400 mM stock solution.

[0038] Preparation of aurecetine: 5 mg aurecetin was diss...

Embodiment 2

[0054] The expression of inflammation-related factors in macrophage RAW264.7 was induced by LPS (4 μg / mL). After the drug acted on the RAW264.7 cells for 3 hours, the cells were collected, the total RNA was extracted, reverse-transcribed into cDNA, and the effect of the drug on the relative expression of inflammatory factors IL-1β and iNOS mRNA was determined by qRT-PCR. The β-actin gene was used as an internal control. The experimental groups are shown in Table 1. Primer sequences are shown in Table 2.

[0055] Results: The activity screening results of inhibiting the expression of macrophage inflammatory factor IL-1βmRNA are shown in the appendix figure 2 . Compared with the model group (group B), the different proportions of fencetin extract (N, O, P group), Qinbing eye drops (Q group), fencetin (group C) and fencetin and fencetine B ( H, J, K, L, M groups) could significantly inhibit the expression of IL-1βmRNA (P image 3 . Compared with the negative control group (g...

Embodiment 3

[0057] The inhibitory effects of each group in Table 3 on the growth of methicillin-resistant Staphylococcus aureus (MRSA) and sensitive Pseudomonas aeruginosa (PA14) were tested. After the MRSA and PA14 strains are activated, streak cultures were carried out on LB solid plates respectively, and cultured at 37°C for 16-18 hours, and the colony size was about 1 mm. Pick 5 colonies and dissolve them in 5ml of MHB liquid medium, vortex and mix well, then add the bacterial solution to a 96-well plate after being diluted 1000 times, 80 μl per well, and add the drug to be tested according to Table 3. Set positive control and negative control simultaneously, the positive control of PA14 bacterial strain is the ciprofloxacin of 0.0625 μ g / ml, the positive control of MRSA bacterial strain is the vancomycin of 1.25 μ g / ml, negative control group does not contain test drug / antibiotic. After incubating at 37°C for 16-18 hours, measure the absorbance value of OD600. The calculation method...

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PUM

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Abstract

The invention relates to traditional Chinese medicine effective components for treating eye diseases, and a composition thereof. The composition contains the effective components including aesculin and aesculin. The invention breaks through the conventional cognition in the field, clarifies the actual effective components and pharmacological action of the Qin ice eye drops, verifies that the composition is stable in action effect and clear in action mechanism, can inhibit the expression of lymphatic neogenesis related factors and inflammatory factors in macrophages, the growth of methicillin-resistant staphylococcus aureus MRSA and pseudomonas aeruginosa PA14 is inhibited, and lymphatic vessel neogenesis caused by mouse corneal alkali burn is inhibited, so that the traditional Chinese medicine effective components and the composition can be used for treating lymphatic vessel neogenesis, inflammation and immune-related eye diseases.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of an active ingredient derived from Qinbing eye drops and an active ingredient composition in the treatment of eye-related diseases. Background technique [0002] Qin Bing Eye Drops, formerly known as Qin Pi Eye Drops, was prescribed by the late Professor Fan Xinfu, a famous Chinese medicine doctor. It was used as an in-hospital preparation in Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine for conjunctivitis, keratitis and electro-optic ophthalmia. For chronic conjunctivitis Symptoms such as red eyes, dry eyes, itchy eyes, burning heat and visual fatigue caused by inflammation have quick onset, no stimulation to cornea and conjunctiva, reduce drug resistance, and reduce recurrence rate. Qin Bing Eye Drops is a pure traditional Chinese medicine eye drop made from Qin Pi and borneol. Modern pharmacological studies have shown that Qinpi h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P27/02A61P29/00A61P31/04A61K31/366
Inventor 张建革周昕林国强刘新泉朱玉莹
Owner SHANGHAI UNIV OF T C M
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