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Novel adipose tissue cryopreservation solution and preparation method thereof

A technique of adipose tissue and cryopreservation, applied in the field of biomedicine, can solve problems such as damaged cells

Inactive Publication Date: 2021-04-20
郑州市和沐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to overcome the problems of thick dendritic ice crystals in cells and excessive peroxide damage caused by oxidative stress when the temperature is lowered during cryopreservation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A new type of adipose tissue cryopreservation solution, the cryopreservation solution contains dimethyl sulfoxide with a volume percentage of 4% to 6%, hydroxyethyl starch with a mass percentage of 0.5% to 10%, and 10% to 10% by mass 20% human albumin.

[0023] A method for preparing adipose tissue, comprising the steps of:

[0024] 1), after washing the adipose tissue sample, move it into a centrifuge tube filled with buffer solution and centrifuge;

[0025] 2), move the adipose tissue to a new centrifuge tube and cut into pieces, add buffer and centrifuge;

[0026] 3) Remove the adipose tissue and mix it with the pre-cooled adipose tissue cryopreservation solution to obtain a mixed solution, which is frozen and then transferred into liquid nitrogen for storage.

Embodiment 2

[0028] A new type of adipose tissue cryopreservation solution, the cryopreservation solution contains dimethyl sulfoxide with a volume percentage of 4% to 6%, hydroxyethyl starch with a mass percentage of 0.5% to 10%, and 10% to 10% by mass 20% human albumin.

[0029] Preferably, the cryopreservation solution contains 4% to 6% by volume of dimethyl sulfoxide, 3.5% to 7% by mass of hydroxyethyl starch and 13% to 17% by mass of human blood white protein.

[0030] Preferably, the solvent of the cryopreservation solution is compound electrolyte injection; the compound electrolyte injection contains sodium chloride 5.26±0.4g / L, sodium gluconate 5.02±0.4g / L, sodium acetate trihydrate 3.68±0.3 g / L, potassium chloride 0.37±0.05g / L, magnesium chloride hexahydrate 0.30±0.05g / L.

[0031] A method for preparing adipose tissue, comprising the steps of:

[0032] 1) After washing the adipose tissue sample, transfer it to a centrifuge tube filled with buffer for centrifugation; the adipose...

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PUM

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Abstract

The invention discloses a novel adipose tissue cryopreservation solution and a preparation method thereof. The novel adipose tissue cryopreservation solution comprises the following components in percentage by volume: 4%-6% of dimethyl sulfoxide, 0.5%-10% of hydroxyethyl starch and 10%-20% of human serum albumin. The cryopreservation method comprises the following steps: 1) cleaning an adipose tissue sample, transferring the adipose tissue sample into a centrifuge tube filled with a buffer solution, and conducting centrifuging; 2) transferring the adipose tissue into a new centrifuge tube, conducting cutting to form fragments, adding a buffer solution, and conducting centrifuging; and 3) moving out the adipose tissue, mixing the adipose tissue with a pre-cooled adipose tissue cryopreservation solution to obtain a mixed solution, freezing the mixed solution, and storing the frozen mixed solution in liquid nitrogen.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an adipose tissue cryopreservation solution and a method for adipose tissue cryopreservation. Background technique [0002] Autologous fat transplantation technology is a technique widely used in aesthetic medicine at present, but the autologous fat particles required for filling have the disadvantages of low survival rate and high absorption rate after transplantation, so a small amount of repeated filling is required for multiple fillings. make up. However, repeated liposuction not only causes pain to patients, but also increases medical costs and risks, so extracorporeal cryopreservation technology emerged as the times require. In cryopreservation technology, the source of tissue, the viability and generation of tissue cells before cryopreservation, the density of cryopreservation, the choice and concentration of cryopreservation liquid protectant, the concentration of FB...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 姜南龙婕
Owner 郑州市和沐生物科技有限公司