A kind of Trichoderma ghana and application thereof
A Ghana Trichoderma, Trichoderma technology, applied in the field of microorganisms, can solve the problems of environmental pollution, resource waste, etc., and achieve the effect of improving quality and increasing production
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Embodiment 1
[0037] This embodiment provides a kind of Trichoderma ghanense, which is collected and isolated from the tomato rhizosphere soil in Sunqiao, Pudong New Area, Shanghai.
[0038] The Trichoderma ghanense bacteria of the present invention is preserved in the General Microorganism Center (CGMCC) of the China Microorganism Culture Collection Management Committee, and the preservation address is: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; preservation date: December 21, 2020 day.
[0039] Culture characteristics of Trichoderma ganae strains:
[0040] The Trichoderma ganamis strain of the present invention is cultivated at 20-30 DEG C for 64 hours under dark conditions in PDA medium, and the colony radius can reach 70 mm, and when cultured at 40 DEG C for 64 hours, the colony radius can reach 50-60 mm. refer to Figure 4 , the conidia were uniformly and continuously spread on the colony, dark green; most of the conidia formed at 35 ℃, and the conidia yield gradual...
Embodiment 2
[0054] The present embodiment provides a preparation method of Trichoderma ganae fermentation supernatant, comprising the following steps:
[0055] Step 1, the Trichoderma ganae species isolated in Example 1 is inoculated in the PDA medium, and cultivated at a constant temperature for one week;
[0056] In step 2, the strains cultivated in step 1 are transferred to the culture solution for culturing in shake flasks for 24 hours to obtain a first-class seed solution;
[0057] Step 3, after sterilizing the culture solution in the intermediate fermentor at a temperature of 121°C and 15 pounds of pressure for 20min, maintaining positive pressure, cooling to 30°C, and then pouring the first-class seed liquid obtained in step 2 into the intermediate fermenter, Pressurized into sterile air, fermented for 6 days at 28-30°C temperature, 0.4-0.5kg pressure, 1500-1800L / H air flow and 120-150rpm rotating speed bar, to obtain Trichoderma fermented liquid;
[0058] Step 4, using a centrifu...
Embodiment 3
[0066] The present embodiment provides an organic water-soluble fertilizer, which corresponds to the Trichoderma ganae fermentation supernatant obtained by adopting group 1-3 culture solution in above-mentioned embodiment 2, respectively including the following (table 2) components by weight percentage:
[0067] Table 2
[0068] component Example 3-1 Example 3-2 Example 3-3 Trichoderma ganae fermentation supernatant 70％ 67.11％ 62.50％ mineral potassium humate 3.43％ 2.75％ 3.25％ Potassium nitrate 3.72％ 8.0％ 2.68％ Urea 3.08％ 3.25％ 8.20％ Ammonium polyphosphate 2.32％ 3.35％ 4.82％ Dipotassium phosphate 6.25％ 6.94％ 6.00％ Molasses Powder 10.70％ 8.50％ 12.05％ carotene 0.5％ 0.1％ 0.5％
[0069] The above-mentioned organic water-soluble fertilizer is prepared by the following method: according to the proportion, put the fermentation supernatant of Trichoderma ganae into the batching tank, then turn on the stirr...
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