Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Strain for producing long-chain dicarboxylic acid and fermentation method of strain

A long-chain dibasic acid and dibasic acid technology, applied in the biological field, can solve the problems of high production cost, low bacterial concentration, and low conversion efficiency

Pending Publication Date: 2021-04-20
CATHAY R&D CENT CO LTD +3
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the first aspect of the present invention is to provide a Candida tropicalis strain, to solve the problems of low cell concentration, long fermentation cycle, and low conversion efficiency in the process of producing decadioic acid by the current Candida tropicalis strain, and then solve the problem of Technical problems with high production costs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Strain for producing long-chain dicarboxylic acid and fermentation method of strain
  • Strain for producing long-chain dicarboxylic acid and fermentation method of strain
  • Strain for producing long-chain dicarboxylic acid and fermentation method of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080]The tropical msr2525 of the present invention is verified by passing experiments, and there has been no significant change in the ability of five passage forms and the production of Tencarbon dibasic acid.figure 1 ,figure 2 ). Under 29 ° C, the first generation and the sixth generation strain were 140.5 g / l and 139.8 g / l (add 4.5 ml of NC10 as a substrate), respectively; colony form: colony surface smooth and humid milk white Glossy, round, neat. It can be seen that the passida tropicalis AM2525 of the tropicalis of the present invention is good. When the decane concentration ranges from 0 to 100 mL / L, the strain Tropicalis AM2525 exhibits a complete and smooth surface, indicating that the strain cell morphology has strong tolerance.

[0081]In order to further verify the tolerance of the bacteria in higher concentration decane, the volume ratio of the bacteria in the decane and seed culture is 10%, 20%, 40%, respectively, no decane The seed medium is a blank control group,...

Embodiment 2

[0084]Take 1 glycerin tube seed to the YPD activation medium, and cultured for 24 h. After 24 h, the culture liquid into seed culture medium (cultured for 20 minutes). Seed medium includes: sucrose 20g / L, corn pulp 2g / L, yeast paste 6g / L, podium phosphate, urea 2 g / L, NC10 1 mL; cultured under 29 ° C for 48 h, measured seed fluid OD620To reach 0.7 (30 times the water), 3.0 ml of seed liquid inoculated into a shake flask equipped with a fermented medium (15 ml of fermentation medium), fermentation medium included: sucrose 30 g / L, corn 5g / L The n-alkane NC10 added in the fermentation medium was 4.5 mL, and the fermentation medium was sterilized at 121 ° C for 20 minutes. Under 29 ° C, fermentation substrates in the fermentation liquid end fermentation were detected at 0, and the period was 160 h.

[0085]After the end of fermentation, the content of DC10 diomeral acid in the fermentation broth was determined was 140 g / L, and the average acid rate was 0.88 g / h · L, the sub...

Embodiment 3

[0087]Take 1 glycerin tube seed to the YPD activation medium, and cultured for 24 h. After 24 h, the culture liquid into seed culture medium (cultured for 20 minutes). Seed medium includes: sucrose 20g / L, corn pulp 2g / L, yeast paste 6g / L, potassium phosphate is 8 g / L, urea 2g / L; medium 48 h under 29 ° C, the OD of the seed fluid620To reach 0.8 (30 times water dilution), 3.0 ml of seed liquid inoculated into a shake flask equipped with a fermentation medium (15 ml of fermentation medium), fermentation medium included: sucrose 30 g / L, corn 5g / L, yeast paste 5g / L, pelletivate potassium dihydrogenate 8g / L, potassium nitrate 4g / L, sodium chloride 1.5 g / L, urea 0.5 g / L. The n-alkane NC10 added in the fermentation medium was 4.5 mL, and the fermentation medium was sterilized at 121 ° C for 20 minutes. At 29 ° C, fermentation substrates in the fermentation substrate were detected at 0, and the period was 180 h.

[0088]After the end of fermentation, the amount of DC10 d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a candida tropicalis strain and a method for producing long-chain dicarboxylic acid by fermenting the strain. The strain is Candida tropicalis Am2525, and the preservation number of the strain is CCTCC NO: M 2019419. The production method of the long-chain dicarboxylic acid comprises the following steps of preparing a seed solution from the candida tropicalis strain Am2525, and / or performing fermentation production of long-chain dicarboxylic acid. Compared with parents, the candida tropicalis Am2525 disclosed by the invention has the advantages that the resistance of the candida tropicalis Am2525 to the toxicity of a substrate decane is enhanced, the productivity of long-chain dicarboxylic acid is improved, the production cost is reduced, the subsequent separation and purification are simple, the fermentation production process is easy to realize large-scale production, and the application prospect is good.

Description

Technical field[0001]The invention belongs to the field of biotechnology, and more particularly to a strain, particularly to a strain producing a long chain diomerate, and a method of fermented long chain diomerate using the strain.Background technique[0002]Long chain diprofen acid is an important raw material for synthetic fragrance, nylon engineering plastic, hot melt, resin, cold-resistant plasticizer, medicine and pesticides, etc. The structural formula is HOOC (CH2)nCOOH, where N is an integer greater than 7. Among them, Tencarbonic acid or 10 carbon long chain diomelic acid, decanic acid, chemical formula is HOOC (CH2)8COOH. As an important monomer raw material, it is widely used to produce polyamide engineering plastics, such as nylon 510, nylon 1010, nylon 610. It can also be used for high temperature lubricants, epoxy resin curing agents, synthetic greases and artificial flavors, cold-resistant plasticizers, etc., are one of the wide chemical raw materials.[0003]At present,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/64C12R1/74
CPCC12N1/16C12N1/165C12R2001/74C12P7/6409C12P7/44
Inventor 汪江林李葳李秦佩姜文晓闵淑雅刘修才
Owner CATHAY R&D CENT CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com