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Streptomyces castanea strain CPAT-W03 and application thereof

A technology of CPAT-W03, Streptomyces chestnutum, applied in the application, bacteria, fungicides, etc., can solve the problems of poor growth, death of the whole plant, economic loss, etc. Short, easy-to-get effects

Active Publication Date: 2021-05-04
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to damage to the stem and root, the tip of the leaf is dry when it is light, the leaves are yellow-green, and the growth is poor, and the whole plant dies when it is severe, causing serious economic losses.

Method used

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  • Streptomyces castanea strain CPAT-W03 and application thereof
  • Streptomyces castanea strain CPAT-W03 and application thereof
  • Streptomyces castanea strain CPAT-W03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Streptomyces chestnutii strain ( Streptomyces badius) Acquisition, identification and preservation of CPAT-W03.

[0044] (1) Streptomyces chestnutii strain ( Streptomyces badius ) Acquisition and identification of CPAT-W03.

[0045] Streptomyces chestnutii bacterial strain described in the present invention ( Streptomyces badius ) CPAT-W03, which is isolated from the coral-like mycorrhiza of Panzhihua cycas. The samples were collected from the Cycad Nature Reserve in Panzhihua City, Sichuan Province, and stored at 4°C for later use. Separation of endophytic actinomycetes from coral-like mycorrhizal mycorrhizal cycas of Panzhihua cycads by plate streaking method: wash the surface soil of coral-like mycorrhiza with distilled water, blot the water, first disinfect with 75% alcohol for 1 min, and then disinfect with 0.1% mercuric chloride solution 5 min, then disinfected with 5% sodium hypochlorite solution for 5 min, and finally rinsed with sterile water 3 times. F...

Embodiment 2

[0054] Streptomyces chestnutii strain ( Streptomyces badius ) Screening of CPAT-W03 strain.

[0055] (1) Screening of strains.

[0056] Preliminary screening of antagonistic bacteria was carried out by cross method. Concrete operation is as follows: take the pathogenic bacterium of stem base rot of Molan ( Phoma arachidicola ) as an indicator bacterium, inoculate a 6 mm agar block of pathogenic bacteria in the center of the improved PDA medium plate, and then inoculate a single colony of bacteria obtained by isolation and culture at an equidistant distance of 2.5 cm from the pathogenic bacteria, inoculate 4 strains per plate, and set 5 replicates After culturing at a constant temperature of 28°C for 5-7 days, observe the size of the inhibition zone and select the strain with the best antagonistic effect.

[0057] (2) Observation of results.

[0058] The isolated strains were screened by the cross method, and the results showed that there were 7 strains with antagonistic ...

Embodiment 3

[0060] Streptomyces chestnutii strain ( Streptomyces badius ) Determination of the biocontrol effect of CPAT-W03 strain.

[0061] (1) Determination of biocontrol effect.

[0062] The confrontation culture method: with the pathogenic bacteria of the stem rot of Molan ( Phoma arachidicola ) as the indicator bacteria, first use a 6mm puncher to take out the activated agar block of pathogenic bacteria, place it on the plate, and then inoculate the antagonistic bacteria by spot connection method, the distance between the two bacteria is 2.5 cm, the control group only inoculates the agar block of pathogenic bacteria, and set 3 Repeat, culture in a 28°C incubator for 5-7 days, observe the growth of pathogenic bacteria, measure the inhibition zone, calculate the inhibition rate, and finally screen out the antagonistic bacteria with the best antagonistic effect. The formula for calculating the bacteriostatic rate: bacteriostatic rate (%) = (control pathogen colony diameter - treatm...

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Abstract

The invention discloses a Streptomyces castanea strain CPAT-W03 and application thereof. The Streptomyces castanea strain 2 is classified and named as Streptomyces badius, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 13, 2020, the preservation number is 19342, and the Streptomyces castanea belongs to the field of bacteria, actinomycetes phylum, actinomycetes class, streptomycete orders, streptomycete families and Streptomyces sp. The application is the application of the Streptomyces castanea strain 2 in biological prevention and control of stem rot of Cymbidium sinense.

Description

technical field [0001] The invention belongs to the technical field of microorganism separation and cultivation, and in particular relates to the application of a Streptomyces chestnutii strain CPAT-W03 in the biological control of stem rot of Molanca spp. Background technique [0002] Cymbidium( Cymbidium sinense ) is a ground plant of the genus Orchid, mainly distributed in China, India, Myanmar, Vietnam, Thailand, Japan Ryukyu Islands and other places. Molan stem base rot is one of the main diseases in artificial cultivation and industrial production. The disease is common and is an important disease on orchids, affecting the growth and viewing of orchids. The disease mainly affects the base of the stem of Molan. In the early stage of the disease, light brown, water-soaked lesions appeared on the roots, and after expansion, they turned brown and rotted, and the base of the stem died. Phoma ( Phoma arachidicola ). Due to damage to the stem and root, the tip of the l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/18A01N63/28A01P3/00C12R1/465
CPCC12Q1/18A01N63/28
Inventor 伍建榕陈健鑫马焕成魏玉倩洪英娣马翔郑艳玲王芳竺永金
Owner SOUTHWEST FORESTRY UNIVERSITY
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