Cultivation method of cordyceps militaris

A cultivation method and technology for Cordyceps militaris are applied in the field of cultivation of Cordyceps militaris, and can solve the problems of low rigidity rate, low yield rate and low content of cordycepin in Cordyceps militaris cultivation technology.

Active Publication Date: 2021-05-11
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of low ossification rate, low weed rate and low content of

Method used

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  • Cultivation method of cordyceps militaris
  • Cultivation method of cordyceps militaris
  • Cultivation method of cordyceps militaris

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specific Embodiment approach 1

[0027] Specific embodiment one: the cultivation method of a kind of Cordyceps militaris of present embodiment, carry out according to the following steps:

[0028] 1. Add the oily soybean meal hydrolyzate, calcium gluconate mother solution and vitamin C mother solution into deionized water, after fully stirring, sterilize and cool to room temperature to obtain a sterilized liquid medium; under sterile conditions, the pupa The mother species of Cordyceps is inoculated in the sterilized liquid medium, and the inoculation amount is 5-10 pieces / 0.5cm 3 , and cultured for 5-7 days at a temperature of 18-22°C and a rotational speed of 140-160r / min to obtain liquid production seeds;

[0029] 2. Under sterile conditions, inoculate the liquid production seeds on the surface of live silkworm chrysalis pupa skin, the inoculation amount is 0.4-0.6mL / one silkworm chrysalis; incubate the live silkworm chrysalis after inoculation at a temperature of 16-20°C in the dark After 20-30 days, the...

specific Embodiment approach 2

[0034] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, the hydrolyzate of oily sage soybean meal, calcium gluconate mother liquor and vitamin C mother liquor are added to deionized water, and after fully stirring, extinguish at 121 ° C. Bacteria for 30 minutes, cooled to room temperature to obtain a sterilized liquid culture medium, the volume ratio of the oily sage soybean meal hydrolyzate, calcium gluconate mother liquor and vitamin C mother liquor to deionized water is 10mL: 2mL: 1.5mL: 1000mL.

[0035] Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0036]Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the hydrolyzed liquid of oily soybean meal is prepared according to the following steps: adding oily soybean meal to a sulfuric acid solution with a concentration of 1.2%, and the solid-to-liquid ratio is 1:8, then reacted at 123°C for 2 hours, and after solid-liquid separation, the initial hydrolyzate was obtained; the initial hydrolyzate was neutralized by calcium carbonate emulsion to pH 3.5, and then decolorized by 1.0% activated carbon for 30 minutes, to obtain Oily Sha soybean meal hydrolyzate, the mass fraction of soluble solids in the oily sand soybean meal hydrolyzate is 10.0%, the mass fraction of total reducing sugar is 8.0%; the total reducing sugar is composed of 80% glucose, 15% arabinose and 5% xylose .

[0037] Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a cultivation method of cordyceps militaris, and relates to the technical field of edible and medicinal fungus cultivation. The method aims at solving the problems that according to an existing cordyceps militaris cultivation technology, the rigidity rate is low, the grass emergence rate is low, and the cordycepin content is low. The method comprises the following steps of inoculating liquid production seeds on the surface of pupa skin of living silkworm chrysalis under a sterile condition, and then culturing for 20-30 days in a dark place; performing opening treatment at the positions of the first pair of small wings of the stiff silkworm chrysalis, performing illumination and temperature difference stimulation for 5-10 days, performing illumination and temperature stimulation on the silkworm chrysalis in a primordium germination stage for 5-6 days, performing illumination and temperature stimulation on the silkworm chrysalis in a sporocarp initial stage for 10-15 days, performing fourth illumination and temperature stimulation on the silkworm chrysalis in a sporocarp growth stage for 3-7 days, obtaining mature sporocarps, and harvesting the sporocarps. The cultivation method of the cordyceps militaris can be obtained.

Description

technical field [0001] The invention relates to the technical field of cultivation of edible and medicinal fungi, in particular to a cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris is also known as Cordyceps militaris and Cordyceps militaris. The host of the wild Cordyceps militaris grows from the top of the parasite pupae from the pupae of insects such as Lepidoptera, which is shaped like a stick, 0.8-3cm long, and orange-yellow. Wild Cordyceps militaris is mainly produced in Liaoning, Jilin, Hebei, Shaanxi, Anhui, Yunnan, Guangdong and Guangxi. [0003] Tussah belongs to Lepidoptera insects and is a characteristic industry in my country. It is now distributed in more than 10 provinces including Liaoning, Jilin, Heilongjiang, Inner Mongolia, Hebei, Shandong, Henan, Guizhou, Sichuan and Hubei. The annual output of tussah silkworm cocoons (chrysalis) in my country accounts for 90% of world output, and tussah silkworm chrysalis is...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20A01G18/40A01G18/50
CPCA01G18/00A01G18/20A01G18/40A01G18/50
Inventor 钱朋智张梅娟
Owner QIQIHAR UNIVERSITY
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