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Blueberry anthocyanin-rich extract and preparation method thereof

A technology rich in anthocyanins and extracts, applied in chemical instruments and methods, food science, azo dyes, etc., to achieve good hypoglycemic activity

Pending Publication Date: 2021-05-11
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the literature proves that blueberries are beneficial to health, there are few preparation methods for anthocyanin-rich blueberry preparations with high hypoglycemic activity, so it is necessary to provide a blueberry anthocyanin extract with high antiglycemic activity

Method used

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  • Blueberry anthocyanin-rich extract and preparation method thereof
  • Blueberry anthocyanin-rich extract and preparation method thereof

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preparation example Construction

[0022] In one embodiment of the present invention, a kind of preparation method of blueberry rich anthocyanin extract is provided, comprises the following steps:

[0023] (1) Mix and immerse frozen blueberries with ethanol acidified with trifluoroacetic acid, beat until uniform, vacuum extract under heating conditions, and then cool to room temperature;

[0024] (2) Evaporating and concentrating the slurry to obtain a concentrated extract after filtering the slurry, adding ethyl acetate to it for mixing, centrifuging and extracting to obtain an aqueous phase extract;

[0025] (3) Purifying the aqueous phase extract with a macroporous resin, eluting with an ethanol solution of trifluoroacetic acid, evaporating the eluent, and freezing to obtain a phenol-rich extract;

[0026] (4) Adsorb the phenol-rich extract with a Sephadex LH-20 packed column, and elute with an ethanol solution. When the colored substance begins to elute from the chromatographic column, collect it in stages ...

Embodiment 1

[0042] (1) Mix and immerse frozen blueberries in ethanol acidified with trifluoroacetic acid, beat until uniform, place at 50°C for vacuum extraction for 2 hours, and cool to room temperature;

[0043] (2) After the slurry is filtered, carry out rotary steaming, evaporate to 1 / 3 of the original volume, and the rotary steaming temperature does not exceed 40°C;

[0044] (3) Mix the obtained concentrated extract with 4 times of ethyl acetate, extract by centrifugation, and obtain the aqueous phase extract;

[0045] (4) Purify the aqueous phase extract with a macroporous resin: elute with 40 column volumes of ethanol solution containing 0.3% trifluoroacetic acid, evaporate the eluent, and freeze to obtain a phenol-rich extract.

[0046] (5) The phenol-rich extract is adsorbed with a Sephadex LH-20 packed column;

[0047] (6) Then elute with 25% ethanol solution (containing 0.3% trifluoroacetic acid). When the colored substance starts to elute from the chromatographic column, coll...

Embodiment 2

[0051] (1) Mix and immerse frozen blueberries and ethanol acidified with trifluoroacetic acid, beat until uniform, place at 40°C for vacuum extraction for 2 hours, and cool to room temperature;

[0052] (2) After the slurry is filtered, carry out rotary steaming, evaporate to 1 / 4 of the original volume, and the rotary steaming temperature does not exceed 40°C;

[0053] (3) Mix the obtained concentrated extract with 5 times of ethyl acetate, and extract by centrifugation to obtain an aqueous phase extract;

[0054] (4) Purify the aqueous phase extract with a macroporous resin: elute with 50 column volumes of ethanol solution containing 0.3% trifluoroacetic acid, evaporate the eluent, and freeze to obtain a phenol-rich extract.

[0055] (5) The phenol-rich extract is adsorbed with a Sephadex LH-20 packed column;

[0056] (6) Then elute with 30% ethanol solution (containing 0.3% trifluoroacetic acid). When the colored substance starts to elute from the chromatographic column, co...

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Abstract

The invention relates to the technical field of blueberry anthocyanin-rich extraction, in particular to a blueberry anthocyanin-rich extract and a preparation method thereof. The preparation method comprises the steps of: mixing frozen blueberries and trifluoroacetic acid acidified ethyl alcohol, immersing the blueberries and carrying out vacuum extraction; filtering the slurry, evaporating and concentrating the slurry to obtain a concentrated extract, adding ethyl acetate, mixing and centrifugally extracting to obtain a water-phase extract; purifying the water-phase extract with macroporous resin, and eluting with an ethanol solution of trifluoroacetic acid to obtain a phenol-rich extract; adsorbing the phenol-rich extract by using a Sephadex LH-20 packed column, eluting by using an ethanol solution, and collecting by stages; intercepting the first 20-30% of the total eluent as an extract sample, and freeze-drying the extract sample to obtain a sample; and dissolving the sample in an aqueous solution containing citric acid, adding caprylic / capric polyethylene glycol glyceride, polyethylene glycol and sodium carboxymethyl cellulose for emulsification, and carrying out vacuum filling or secondary freeze-drying and sealing after homogenization. The total anthocyanin content in the obtained blueberry anthocyanin-rich extract is more than 20 times that of freeze-dried fruit powder, and the blueberry anthocyanin-rich extract has good hypoglycemic activity.

Description

technical field [0001] The invention relates to the technical field of blueberry anthocyanin-rich extraction, in particular to a blueberry anthocyanin-rich extract and a preparation method thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Type 2 diabetes currently affects the health of many people around the world. In addition to traditional treatments that rely on insulin injections or prescription drugs, natural products, especially berry preparations, have been used as one of the alternative medicinal treatments for diabetes. [0004] Blueberries and related fruits are especially nutritious and have been shown to be beneficial for a variety of conditions. Most ...

Claims

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Application Information

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IPC IPC(8): C07D311/62C09B61/00C09B67/54A23L33/105
CPCC07D311/62C09B61/00C09B67/0096A23L33/105A23V2002/00A23V2200/328A23V2250/2104
Inventor 孙锐韩燕苓杨明冠
Owner QILU UNIV OF TECH
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