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Nanosecond pulse laser perforation system and method based on spatial light modulation technology

A nanosecond pulsed laser and spatial light modulation technology, applied in the field of nanosecond pulsed laser perforation system, can solve the problems of high cost and complicated operation of cell photoperforation, and achieve the effect of increasing perforation speed and flux and protecting high-speed imagers.

Active Publication Date: 2021-05-11
XI AN JIAOTONG UNIV
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Problems solved by technology

However, the use of two pulsed lasers to induce jet formation to achieve cell photoporation has the disadvantages of high cost and complicated operation.

Method used

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  • Nanosecond pulse laser perforation system and method based on spatial light modulation technology
  • Nanosecond pulse laser perforation system and method based on spatial light modulation technology
  • Nanosecond pulse laser perforation system and method based on spatial light modulation technology

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Embodiment Construction

[0038] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0039] Such as figure 1 As shown, a nanosecond pulse laser perforation system based on spatial light modulation technology includes a pulse laser 1 , a spatial light modulator 9 , a white light source 12 and a system control storage module 19 .

[0040] A beam splitting prism 2, a half-wave plate 4, a concave lens 5, a second convex lens 6 and a first reflector 7 are sequentially arranged in the pulse laser propagation direction of the pulse laser 1; the transmission and reflection splitting ratio of the beam splitting prism 2 is 9 : 1; the image focus of the concave lens 5 coincides with the object focus of the second convex lens 6; the angle between the first reflector 7 and the incident pulse light is 45° counterclockwise; the energy meter 3 is placed on the reflected light direction of th...

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Abstract

The invention provides a nanosecond pulse laser perforation system and method based on a spatial light modulation technology. A spatial light modulator is used for modulating a spatial light field of single-pulse laser; the modulated pulse laser is focused by an objective lens to form controllable micro-jet in a micro-fluidic chip, and the high-speed micro-jet is used for perforating cells and introducing exogenous substances. The novel cell membrane micro-surgery method has the potential of accurately controlling the introduction dosage and improving the perforation efficiency and the success rate.

Description

technical field [0001] The present invention relates to the fields of liquid photo-induced breakdown and cell photo-perforation, in particular to a nanosecond pulse laser perforation system and method based on spatial light modulation technology. Background technique [0002] Cell membrane microsurgery has great scientific significance for us to deeply analyze the life process and the pathogenesis of diseases. For example, introducing some exogenous biomacromolecules such as protein, DNA, RNA and siRNA into living cells can interfere with protein function expression Even targeted killing or reversal of diseased cells in the microscopic field. In this process, how to achieve recoverable perforation of cells without affecting cell activity as much as possible is a key step. [0003] In the process of introducing exogenous substances into cells, the ability to actively introduce them is the key to improving the introduction efficiency, success rate, and dose controllability. A...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/36C12M1/34C12M1/00H01S3/00
CPCC12M23/16C12M35/00C12M41/46C12M41/48C12N15/87C12N13/00H01S3/0085
Inventor 付磊张镇西王晶姚翠萍王斯佳王萍辛静张璐薇
Owner XI AN JIAOTONG UNIV
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