Method for constructing high-quality porcine nuclear transplantation donor cells with high lean meat percentage, fast growth, high fertility and resistance to series epidemic diseases and application thereof

A technology of recombinant cells and plasmids, applied in the biological field, can solve problems such as inability to infect live pigs, inactivation of receptor proteins, and reduced infectivity.
CN112779291AActive Publication Date: 2021-05-11NANJING KGENE GENETIC ENG CO LTD
4 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING KGENE GENETIC ENG CO LTD
Publication Date
2021-05-11

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for constructing high-quality porcine nuclear transplantation donor cells with high lean meat percentage, fast growth, high fertility and resistance to series epidemic diseases and application thereof. A CRISPR / Cas9 system for editing five genes of porcine MSTN, SST, INHA, CD163 and pAPN comprises a Cas9 expression vector and gRNA expression vectors respectively aiming at the porcine MSTN, SST, INHA, CD163 and pAPN genes; and the plasmid complete sequence of the Cas9 expression vector is shown as SEQ ID NO. 2. The corresponding gRNA expression vectors are respectively designed aiming at different target points of the MSTN, SST, INHA, CD163 and pAPN genes, and gRNA with higher editing efficiency and expression vectors thereof are obtained through screening. The gene editing is performed by matching with the modified Cas9 high-efficiency expression vector, and the editing efficiency is obviously improved compared with that of the original vector.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The present invention belongs to the biotechnology field, and the CRISPR / CAS9 system for MSTN, SST, INHA, CD163 and PAPN-five genes and its high growth rate, high growth, high reproductive and anti-blue Application of Quality Pig Nuclear Transplantation Differential Cells in Diarrhea.Background technique

[0002] Pig is one of the earliest domestic livestock in my country, which has always been an important meat animal in the long river of history. Chinese people love pork, related to diet culture for thousands of years. Since 2000, pork has more than 70% of meat consumption in my country, and is the most important consumption meat in my country. At present, the price of urban lean meat in my country has exceeded the price of more than 1 times, with more than 60% of lean-lean pigmented pigs, and the lean meat-lean sex has a further improvement space. On the other hand, the current domestic hyperfection pig has a weight of about 120kg. The pig is only 180 days from...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More