Drug combination composition for type 2 diabetes and liver injury and application of drug combination composition

A type 2 diabetes and combined drug technology, applied in the field of biomedicine, can solve the problems that the gain effect has not been further verified, and there is no detailed report

Active Publication Date: 2021-05-18
JIANGSU DAYSEBIOTECH LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing studies have confirmed that bifidobacteria and selenium have a certain hypoglycemic effect, but whether the combination of selenium-enriched bifidobacterium longum and pioglitazone has a gain effect has not been further verified, and there is no detailed report. Therefore, the present invention is devoted to Study on the alleviating effect of bifidobacterium selenium longum combined with pioglitazone on type 2 diabetes and liver injury

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  • Drug combination composition for type 2 diabetes and liver injury and application of drug combination composition
  • Drug combination composition for type 2 diabetes and liver injury and application of drug combination composition
  • Drug combination composition for type 2 diabetes and liver injury and application of drug combination composition

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Effect test

Embodiment 12

[0075] Example 1 Construction of type 2 diabetes mouse model and combined drug detection

[0076] 1. Preparation of Se-enriched Bifidobacterium longum DD98

[0077] RCM modified medium: beef extract 1%, peptone 0.5%, yeast powder 0.3%, glucose 0.6%, sodium chloride 0.5%, anhydrous sodium acetate 0.3%, L-cysteine ​​hydrochloride 0.05%, agar 1.5 -1.8%.

[0078] Preparation method: inoculate the Bifidobacterium longum DD98 strain (the preservation number of the Bifidobacterium longum DD98 strain is CGMCC No. 16573) into the RCM modified medium, and after anaerobic cultivation at 37°C for 12 hours, add selenium to the medium Na2SO4 (concentration: 18.5 μg / mL), continued to cultivate for 24 hours, kept the pH value at 6.8, and fermented in a high-selenium environment to prepare selenium-enriched DD98 fermentation broth.

[0079] The detection method of selenium content in Se-DD98 fermentation broth refers to the national standard GB5009.93-2010 "Determination of Selenium in Food"...

Embodiment 2

[0082] Embodiment 2 Changes in food intake and body weight of mice

[0083] The food intake of the mice was detected every week, and the average daily food intake of the mice in each group was calculated during the administration period. After the mice were fasted for 12 hours, the fasting body weight of the mice was weighed. figure 1 is the detection result of the food intake of the mice, figure 2 It is the result of fasting weight change of mice.

[0084] Depend on figure 1 It can be seen that the food intake of the mice in the T2DM group was significantly increased compared with that in the NC group (p<0.05), which was in line with the characteristics of diabetic polyphagia. Compared with the T2DM group, the food intake of the mice in each administration group was reduced. Compared with the PIO group, the food intake of the PIO+Se group and the PIO+DD98 group did not decrease significantly, but the combination of selenium-enriched DD98 and pioglitazone could significan...

Embodiment 3

[0087] Example 3 Changes in Fasting Blood Glucose in Mice

[0088] The mice were fasted for 12 hours, and the blood was collected from the tail to detect the fasting blood glucose, and the blood glucose was detected every week during the 3 weeks of administration. image 3 It is the change result of fasting blood glucose (FBG) of mice.

[0089] Depend on image 3 It can be seen that the fasting blood glucose value of the mice after modeling is greater than 11.1mmol / L, which proves that the type 2 diabetes model is successful. There was no significant difference in the fasting blood glucose of the mice in each model group when grouping, and the fasting blood glucose of the mice with type 2 diabetes continued to rise after 1 week of administration. Compared with the T2DM group, the PIO group, PIO+Se group, PIO+DD98 group, PIO group The increase in fasting blood glucose in the +Se-DD98 group was alleviated, and at the second week of administration, the fasting blood glucose in al...

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Abstract

The invention provides a drug combination composition for type 2 diabetes and liver injury and an application of the drug combination composition, and belongs to the field of biological medicine. The drug combination composition comprises selenium-enriched probiotics and thiazolidinedione medicine pioglitazone, and experiments of a type 2 diabetes mouse model constructed by combining high-fat feeding with STZ injection find that after the selenium-enriched probiotics DD98 and the pioglitazone are combined for administration, the weight and fasting blood glucose of the mouse can be obviously improved, liver lipid deposition, oxidative stress and inflammation levels can be reduced, and liver tissue damage can be relieved, so that the administration dosage of the pioglitazone is reduced, and a basis is provided for clinically avoiding side effects of the pioglitazone.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a combination medicine composition for type 2 diabetes and liver injury and application thereof. Background technique [0002] Diabetes is a metabolic disease characterized by hyperglycemia. Long-term hyperglycemia can lead to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. Diabetes is one of the most important chronic non-communicable diseases in the world, and is an important global health problem. The global diabetes map released by the International Diabetes Federation in 2017 shows that the number of people with diabetes has reached 425 million, of which type 2 diabetes is diabetes main types of . In recent years, with the development of socio-economic level, changes in population structure and changes in the lifestyle of the whole people, the incidence of diabetes has been increasing year by year. China has becom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K35/741A61K35/745A61K31/4439A61P3/10A61P1/16
CPCA61K45/06A61K35/741A61K35/745A61K31/4439A61P3/10A61P1/16A61K2300/00Y02A50/30
Inventor 陈代杰高斐陆惠刚朱慧陆春怡钱志祥
Owner JIANGSU DAYSEBIOTECH LTD
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