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Efficacy and application of lactobacillus plantarum YZX21capable of reducing blood sugar

A Lactobacillus plantarum and blood sugar technology, applied in the field of food microorganisms, can solve the problems of lack of screening high-yield GLP-1, lack of probiotics to regulate GLP-1 effect verification and mechanism reveal, etc., to achieve good blood sugar regulation effect and good intestinal survival. Ability, the effect of no pollution to the environment

Inactive Publication Date: 2021-05-28
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The bottleneck of the above problems lies in: the lack of an effective method for screening high-yield GLP-1 and adapting to the human intestinal environment, and the lack of verification of the effect and mechanism of probiotics regulating GLP-1

Method used

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  • Efficacy and application of lactobacillus plantarum YZX21capable of reducing blood sugar
  • Efficacy and application of lactobacillus plantarum YZX21capable of reducing blood sugar
  • Efficacy and application of lactobacillus plantarum YZX21capable of reducing blood sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. The hypoglycemic efficacy of Lactobacillus plantarum YZX21

[0050] (1) Lactobacillus plantarum YZX21 has excellent gastrointestinal tract survival ability

[0051] Has excellent surface hydrophobicity and self-aggregation ability

[0052] Compared with the model strain Lactobacillus rhamnosus GG (LGG), the surface hydrophobicity of Lactobacillus plantarum YZX21 reached 53.35±1.49%; the self-aggregation ability reached 94.13±0.02%. Therefore, it has excellent adhesion (see Figure 2a ).

[0053] Has excellent acid and bile salt resistance

[0054] Plantarum YZX21 can not only survive well after being digested in an acidic environment with a pH of 3.00 for 3 hours, but also achieve a certain degree of growth, up to 108.74±0.021%; After 4 hours of digestion, the survival rate is as high as 76.62±6.11%, and it has excellent acid and bile salt resistance (see Figure 2b ).

[0055] Excellent ability to tolerate simulated gastrointestinal fluid

[00...

Embodiment 2

[0061] Example 2. Mechanism of hypoglycemic effect of Lactobacillus plantarum YZX21

[0062] We observed a significant difference in the metabolites between the diabetes group and the Lactobacillus plantarum YZX21 group in the OPLS-DA scoring chart ( Figure 6a ), which suggested that there were significant differences between the two intestinal metabolic profiles. Figure 6b A total of 88 metabolites were significantly different between the diabetic group and the L. plantarum YZX21 intervention group. Based on the VIP score and the fold-of-difference value, we found that free fatty acids were highly correlated with diabetes, which was 2.2 times that of the Lactobacillus plantarum YZX21 supplemented group. Among the top ten differential products, the fifth place, β-hydroxybutyric acid, is closely related to free fatty acids. And the β-hydroxybutyric acid in the Lactobacillus plantarum YZX21 intervention group increased significantly, which was more than 3 times higher than t...

Embodiment 3

[0064] Embodiment 3. A kind of preparation method of hypoglycemic probiotic fermented yogurt, described preparation method comprises:

[0065] (1) Culture Lactobacillus plantarum YZX21 in fresh milk or reduced milk at 37°C for 24-36 hours for later use;

[0066] (2) Add the bacterial solution obtained in step 1 into sterilized pure milk, stir well and mix evenly, and then leave it to ferment at 37-42°C for 8-12 hours to prepare probiotic fermented yogurt with hypoglycemic effect.

[0067] (3) After the probiotic fermented yoghurt was diluted and counted on the plate, the live bacteria of Lactobacillus plantarum YZX21 was greater than 1×10 9 CFU / g.

[0068] The invention only requires a single bacterial strain to have a good blood sugar regulation effect, so it can be used as an excellent microbial preparation replacing traditional medicines to regulate blood sugar levels and type 2 diabetes. Compared with the existing technology, this case combines the in vitro (gastrointes...

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Abstract

The invention belongs to the technical field of food and medicine microorganisms, and discloses efficacy and application of probiotics capable of reducing blood sugar. The efficacy of the probiotic product capable of reducing blood sugar comprises the blood sugar reducing effect of the lactobacillus plantarum YZX21 and the blood sugar reducing mechanism of the lactobacillus plantarum YZX21. The application of the probiotic product capable of reducing blood sugar comprises the step of preparing lactobacillus plantarum YZX21 yoghourt. The probiotics provided by the invention have an excellent blood sugar regulating effect and a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of food microorganisms, and in particular relates to a preparation method and application of hypoglycemic probiotics. Background technique [0002] Type 2 diabetes is characterized by elevated levels of glucose in the blood. Hyperglycemia is the third largest independent risk factor for premature mortality and disability in individuals. The hormone GLP-1 secreted by intestinal L cells can stimulate insulin secretion, inhibit gastric emptying, and control blood sugar stability. Current research is focusing on increasing endogenous GLP-1 production and secretion. Most people with type 2 diabetes have insulin resistance. Insulin resistance leads to elevated levels of circulating triglycerides, leading to elevated levels of free fatty acids, their hydrolysates. Long-term free fatty acid stimulation or long-term high-fat diet can lead to impairment of the secretion and function of GLP-1. Since more than half...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P3/10A23C9/123C12R1/25
CPCA61K35/747A61P3/10A23C9/123A23V2002/00A23V2400/169A23V2200/328
Inventor 张兰威张喆刘同杰易华西公丕民梁曦吕优优
Owner OCEAN UNIV OF CHINA
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