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Method for rapidly identifying authenticity of hulless oat flour and buckwheat flour

A technology of naked oat flour and buckwheat flour, which is applied in the rapid identification of the authenticity of buckwheat flour and in the field of naked oat flour, can solve the problems of lack of intuition and accuracy, high equipment investment and high quality testing personnel, and achieve simple operation and economical use of instruments Affordable effect

Pending Publication Date: 2021-06-01
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is an authenticity identification method for oat flour and buckwheat flour in China, the patent publication number is CN105223164B. This method uses chemometric software to establish an identification model for the NIR diffuse reflectance spectrum fingerprint of the sample, and uses the model to identify the authenticity of the wheat flour to be inspected. The equipment input and the quality of testing personnel are required to be relatively high, and at the same time, there is also a lack of intuition and accuracy

Method used

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  • Method for rapidly identifying authenticity of hulless oat flour and buckwheat flour
  • Method for rapidly identifying authenticity of hulless oat flour and buckwheat flour
  • Method for rapidly identifying authenticity of hulless oat flour and buckwheat flour

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Detection of oat flour mixed with wheat flour:

[0038] 1) Use a toothpick to randomly pick a small amount (1-2mg) of test sample powder on a glass slide;

[0039] 2) Add 2-3 drops of acetic acid glycerin solution (dispersant);

[0040] 3) After the sample is fully dispersed on the glass slide, add a cover glass;

[0041] 4) Absorb excess solution on the glass slide with filter paper;

[0042] 5) Place the prepared glass slide under a microscope, adjust the magnification to 40×10, observe the starch granules in the sample and take pictures, and measure the naked oat flour samples doped with 10%, 20%, and 30% wheat flour respectively, Microscopic images obtained as Figure 5 shown.

[0043] 6) Analysis and testing results: compare the microscope pictures of pure naked oats and pure wheat starch granules; it can be seen from the microscope pictures of the samples that oblate and spherical large grains of wheat starch are mixed in the polygonal and elliptical naked oat...

Embodiment 2

[0045] Detection of buckwheat flour mixed with wheat flour:

[0046] 1) Use a toothpick to randomly pick a small amount (1-2mg) of test sample powder on a glass slide;

[0047] 2) Add 2-3 drops of acetic acid glycerin solution (dispersant);

[0048] 3) After the sample is fully dispersed on the glass slide, add a cover glass;

[0049] 4) Absorb excess solution on the glass slide with filter paper;

[0050] 5) Place the prepared glass slide under the microscope, adjust the magnification to 40×10, observe the starch granules in the sample and take pictures, and measure the buckwheat flour samples doped with 10%, 20%, and 30% wheat flour respectively, Microscopic images obtained as Image 6 shown.

[0051] 6) Analysis and testing results: compare the microscope pictures of pure buckwheat and pure wheat starch granules; from the sample microscope pictures, it can be seen that irregular polyhedral spherical buckwheat starch granules are mixed with oblate and spherical large-gra...

Embodiment 3

[0053] Detection of cornstarch mixed with naked oat flour:

[0054] 1) Use a toothpick to randomly pick a small amount (1-2mg) of test sample powder on a glass slide;

[0055] 2) Add 2-3 drops of acetic acid glycerin solution (dispersant);

[0056] 3) After the sample is fully dispersed on the glass slide, add a cover glass;

[0057] 4) Absorb excess solution on the glass slide with filter paper;

[0058] 5) Place the prepared glass slide under a microscope, adjust the magnification to 40×10, observe the starch granules in the sample and take pictures, and measure the naked oat flour samples doped with 10%, 20%, and 30% corn starch respectively , the obtained microscopic image is as Figure 7 shown.

[0059] 6) Analysis and testing results: compare the microscope pictures of pure naked oats and pure corn starch granules; from the microscope pictures of the samples, it can be seen that there are irregular and polyhedral large grains of corn starch mixed in the polygonal and...

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Abstract

The invention relates to a method for rapidly identifying the authenticity of hulless oat flour and buckwheat flour. The method comprises the following steps: S1, preparing a dispersing agent; S2, preparing a standard sample: respectively taking pure hulless oat flour and pure buckwheat flour as standard samples, taking a trace of standard samples on a glass slide, dropwise adding 2-3 drops of a dispersing agent, dispersing, adding a cover glass, absorbing redundant solution, placing the glass slide under a biological microscope, and carrying out microscopic observation and photographing to obtain a standard sample microscope picture; S3, performing sample detection: obtaining a microscope picture of the to-be-detected sample according to the same method in step S2; and S4, performing sample analysis: carrying out microscope picture comparison, if the starch particle shape and the particle size of the to-be-detected sample are consistent with the corresponding characteristics of the two standard samples, determining that the to-be-detected sample is pure hulless oat flour or pure buckwheat flour, and if not, determining that the to-be-detected sample is an adulterated product. The method for identifying the authenticity of the hulless oat flour and the buckwheat flour by identifying the shape and the granularity of the starch granules has the advantages of accuracy, intuition, rapidness, simplicity and convenience in operation, economical and practical used instruments and the like.

Description

technical field [0001] The invention relates to the field of food, in particular to a method for quickly identifying the authenticity of naked oat flour and buckwheat flour. Background technique [0002] Naked oats (also known as naked oats, oats) have extremely high nutritional value. Protein is the most important building material for the human body. The protein content in naked oats ranks first among grains. Among them, the 8 kinds of amino acids necessary for the human body are complete and the ratio is reasonable. The human body has high digestion and absorption rate and utilization rate; the average fat content of naked oats is It is 6.3%, ranking first among grains, and belongs to high-quality vegetable fat; naked oats also contain a lot of vitamins, including B vitamins, folic acid, vitamin H, pantothenic acid, etc., especially vitamin E, which contains 15mg per 100 grams; Compared with grains, the content of phosphorus, calcium and iron in naked oats is the first a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N15/14G01N15/02G01N1/28
CPCG01N21/84G01N15/0205G01N15/1434G01N1/2813G01N2021/8466
Inventor 刘英王展李芳
Owner WUHAN POLYTECHNIC UNIVERSITY