Composite microbial agent, preparation of composite microbial agent, and application of composite microbial agent in rapid reduction of ammonia gas and hydrogen sulphide in farm environment
A compound microbial inoculant, Lactobacillus plantarum technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low ammonia and hydrogen sulfide removal effect, falsely high strain concentration, damage to body functions, etc. problems, to achieve excellent reduction and removal effects, broad application prospects, and remarkable effects
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Embodiment 1
[0054] Composite microbial bacterial agent, which contains the following raw materials in the number of bacterial cells:
[0055] Unusual Wickham's yeast or fermentation broth containing it3;
[0056] Lactobacillus plantarum or its fermented liquid 1;
[0057] The total number of bacteria in the compound microbial agent is ≥ 1×10 9 unit / ml, the Latin name of abnormal Wickham yeast is Wickerhamomyces anomalus, and its preservation number is CGMCC No.20654; the Latin name of Lactobacillus plantarum is lactobacillus plantarum, and its preservation number is CGMCC No.20656.
[0058] The preparation method of the composite microbial bacterial agent is to use the abnormal Wickham yeast with the Latin name of Wickerhamomyces anom alus and the preservation number of CGMCC No.20654, and the plant milk with the Latin name of lactobacillus plantarum and the preservation number of CGMCC No.20656 The bacilli are respectively inoculated in a sterilized medium containing carbon sources, ni...
Embodiment 2
[0070] The difference between this embodiment and embodiment 1 is:
[0071] Composite microbial bacterial agent, which contains the following raw materials in the number of bacterial cells:
[0072] Unusual Wickham's yeast or a fermentation broth containing it1;
[0073] Lactobacillus plantarum or the fermentation liquid containing it3;
[0074] The preparation method of the composite microbial bacterial agent is to inoculate Wickham abnormalities yeast and plantarum lactobacillus respectively in sterilized culture medium containing carbon source, nitrogen source and inorganic salt for aerated culture to make fermentation liquid, and the above-mentioned The fermentation broth is compounded in proportion or purified in proportion.
[0075] Above-mentioned preparation method comprises following processing steps:
[0076] (1) Activation of bacteria
[0077] Inoculate the abnormal Wickham yeast and Lactobacillus plantarum on the corresponding sterile liquid medium, culture to ...
Embodiment 3
[0086] The difference between this embodiment and embodiment 1 is:
[0087] Composite microbial bacterial agent, which contains the following raw materials in the number of bacterial cells:
[0088] Unusual Wickham's yeast or fermentation broth containing it 1.5;
[0089] Lactobacillus plantarum or its fermented liquid 1;
[0090] The preparation method of the composite microbial bacterial agent is to respectively inoculate the abnormal Wickham yeast and plantarum lactobacillus in the sterilized medium containing carbon source, nitrogen source and inorganic salt for aerated culture to make a fermentation liquid, and the above-mentioned The fermentation broth is compounded in proportion or purified in proportion.
[0091] Above-mentioned preparation method comprises following processing steps:
[0092] (1) Activation of bacteria
[0093] Inoculate the abnormal Wickham yeast and Lactobacillus plantarum on the corresponding sterile liquid medium, culture to the logarithmic ph...
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