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Composite microbial agent, preparation method thereof, and application of composite microbial agent in rapid deodorization and purification of high-concentration wastewater in livestock and poultry farms

A compound microbial inoculant, Lactobacillus plantarum technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor treatment effect of livestock and poultry breeding wastewater, single bacterial structure, etc., and achieve excellent Subtraction removal effect, good effect, effect of reducing pollution

Active Publication Date: 2021-06-25
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many kinds of sewage treatment bacterial agents on the market, they are mainly used in domestic sewage, industrial wastewater and other fields, and there are few for livestock and poultry breeding sewage, and the bacteria have a single structure, which is suitable for some easy-to-treat sewage It can have a good effect, but the treatment effect of livestock and poultry breeding wastewater with complex components is not good

Method used

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  • Composite microbial agent, preparation method thereof, and application of composite microbial agent in rapid deodorization and purification of high-concentration wastewater in livestock and poultry farms
  • Composite microbial agent, preparation method thereof, and application of composite microbial agent in rapid deodorization and purification of high-concentration wastewater in livestock and poultry farms
  • Composite microbial agent, preparation method thereof, and application of composite microbial agent in rapid deodorization and purification of high-concentration wastewater in livestock and poultry farms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Composite microbial bacterial agent, which contains the following raw materials in the number of bacterial cells:

[0055] Unusual Wickham's yeast or fermentation broth containing it3;

[0056] Lactobacillus plantarum or fermentation broth containing it1;

[0057] Bacillus amyloliquefaciens or a fermentation broth containing it1;

[0058] The total number of bacteria in the compound microbial agent is ≥ 1×10 9 individual / ml, the Latin name of abnormal Wickham yeast is Wickerhamomyces anomalus, and its preservation number is CGMCC No.20654; the Latin name of Lactobacillus plantarum is lactobacillus plantarum, and its preservation number is CGMCC No.20656; The Latin name of the bacillus is Bacillus amyloliquefaciens, and its preservation number is CGMCC No.1.7463.

[0059] The preparation method of composite microbial inoculum comprises the following process steps:

[0060] (1) Activation of bacteria

[0061] The abnormal Wickham yeast with the Latin name Wickerhamom...

Embodiment 2

[0070] The difference between this embodiment and embodiment 1 is:

[0071] Composite microbial bacterial agent, which contains the following raw materials in the number of bacterial cells:

[0072] Unusual Wickham's yeast or fermentation broth containing it1;

[0073] Lactobacillus plantarum or fermentation broth containing it3;

[0074] Bacillus amyloliquefaciens or a fermentation broth containing it 2.

[0075] The preparation method of composite microbial inoculum comprises the following process steps:

[0076] (1) Activation of bacteria;

[0077] (2) Expanded culture of strains;

[0078] (3) Strain compounding.

[0079] The activation culture conditions of abnormal Wickham yeast in the step (1) are: temperature 28° C., rotating speed 160 rpm shaker culture to the logarithmic phase of growth; the activation culture conditions of Lactobacillus plantarum are: temperature 30°C, 160 rpm shaker culture to the logarithmic phase of growth; the activation culture conditions ...

Embodiment 3

[0083] The difference between this embodiment and embodiment 1 is:

[0084] Composite microbial bacterial agent, which contains the following raw materials in the number of bacterial cells:

[0085] Unusual Wickham's yeast or fermentation broth containing it 1.5;

[0086] Lactobacillus plantarum or fermentation broth containing it 1.5;

[0087] Bacillus amyloliquefaciens or a fermentation broth containing it 1.2.

[0088] The preparation method of composite microbial inoculum comprises the following process steps:

[0089] (1) Activation of bacteria;

[0090] (2) Expanded culture of strains;

[0091] (3) Strain compounding.

[0092] The activation culture conditions of the abnormal Wickham yeast in the step (1) are as follows: the temperature is 32° C., and the rotation speed is 160 rpm, and the shaking table is cultivated to the logarithmic phase of growth; the activation culture conditions of Lactobacillus plantarum are: temperature 33°C, 160 rpm shaker culture to the lo...

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Abstract

The invention relates to a microbial strain and application thereof, in particular to a compound microbial agent, a preparation method thereof, and application of the compound microbial agent in rapid deodorization and purification of high-concentration wastewater in livestock and poultry farms. The invention provides a compound microbial agent containing Wickerhamomyces anomalus, lactobacillus plantarum and bacillus amyloliquefaciens, and a preparation method thereof. The microbial agent can effectively reduce the release amount of NH3 and H2S in breeding sewage of livestock and poultry farms; The content of COD, ammonia nitrogen and total phosphorus in livestock and poultry farm breeding wastewater treated by the compound microbial agent can meet the requirements of relevant national standards in a short time, the treatment period of the breeding wastewater is greatly shortened, and the compound microbial agent has a wide application prospect.

Description

technical field [0001] The invention relates to microbial strains and applications thereof, in particular to a composite microbial bacterial agent and its preparation and application in rapid deodorization and purification of high-concentration wastewater from livestock and poultry farms. Background technique [0002] Wastewater from livestock and poultry breeding contains a large amount of pollutants, which far exceeds the self-purification ability of the environment. Direct discharge into the environment will cause a series of environmental problems such as water pollution, soil pollution and air pollution, which will affect the ecological environment, animal and plant survival and Hazard to human health. At present, the discharge of COD, nitrogen, phosphorus and other pollutants in livestock and poultry wastewater exceeds that of industrial wastewater and domestic wastewater, and agricultural pollution sources have become the top three pollution sources including industri...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/20C02F3/34C12R1/645C12R1/25C12R1/07
CPCC12N1/16C12N1/20C02F3/34C02F3/347C02F2103/20C02F2209/08C02F2209/14C02F2209/18C02F2209/26
Inventor 胡栋王占武王旭彭杰丽张翠绵贾楠马佳
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI