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Composite microbial agent and its preparation and its application in rapid reduction of ammonia and hydrogen sulfide in farm environment

A compound microbial inoculant and microorganism technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low removal effect of ammonia and hydrogen sulfide, falsely high concentration of strains, instability, etc. Excellent subtraction and removal effect, broad application prospects, and the effect of enhancing the effect

Active Publication Date: 2022-06-28
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ammonia and hydrogen sulfide in the stench are soluble or easily soluble in water. These harmful gases are easily adsorbed on the mucous membranes of the respiratory system of livestock and even humans. If animals are exposed to and live in harmful gas environments for a long time, the function of the immune system will be impaired. Weakened, the function of the body will be irreversibly damaged, resulting in a slow decline in production performance
The stench problem of farms has always been the focus and difficulty of pollution prevention and control, which restricts the green and healthy development of the livestock and poultry breeding industry
Although the biological deodorization method based on the application of environmental microorganisms is the current development direction, most of the bacterial agents currently on the market have a falsely high concentration of bacterial strains, and the removal effect of ammonia and hydrogen sulfide in the farm environment is low and unstable. question

Method used

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  • Composite microbial agent and its preparation and its application in rapid reduction of ammonia and hydrogen sulfide in farm environment
  • Composite microbial agent and its preparation and its application in rapid reduction of ammonia and hydrogen sulfide in farm environment
  • Composite microbial agent and its preparation and its application in rapid reduction of ammonia and hydrogen sulfide in farm environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A composite microbial inoculum, which contains the following raw materials in the number of parts:

[0055] Abnormal Wickham yeast or fermentation broth containing it3;

[0056] Lactobacillus plantarum or fermentation broth 1 containing the same;

[0057] The total number of bacteria in the compound microbial inoculum is ≥1×10 9 per ml, the Latin name of Wickham anomaly is Wickerhamomyces anomalus, and its deposit number is CGMCC No. 20654; the Latin name of Lactobacillus plantarum is lactobacillus plantarum, and its deposit number is CGMCC No. 20656.

[0058] The preparation method of the compound microbial inoculum is to combine the abnormal Wickham yeast with the Latin name of Wickerhamomyces anom alus and the deposit number of CGMCC No.20654, and the vegetable milk of the Latin name of lactobacillus plantarum with the deposit number of CGMCC No.20656. The bacilli are respectively inoculated into the sterilized medium containing carbon source, nitrogen source and i...

Embodiment 2

[0070] The difference between this embodiment and Embodiment 1 is:

[0071] A composite microbial inoculum, which contains the following raw materials in the number of parts:

[0072] Abnormal Wickham yeast or fermentation broth containing it 1;

[0073] Lactobacillus plantarum or its fermentation broth 3;

[0074] The preparation method of the composite microbial inoculum is to inoculate the abnormal Wickham yeast and Lactobacillus plantarum respectively in the culture medium after sterilization and containing carbon source, nitrogen source and inorganic salt to carry out aeration culture to make fermentation liquid, and the above-mentioned The fermentation broth is compounded in proportion or purified in proportion.

[0075] The above-mentioned preparation method comprises the following processing steps:

[0076] (1) Strain activation

[0077] The abnormal Wickham yeast and Lactobacillus plantarum are respectively inoculated on the corresponding sterile liquid medium, an...

Embodiment 3

[0086] The difference between this embodiment and Embodiment 1 is:

[0087] A composite microbial inoculum, which contains the following raw materials in the number of parts:

[0088] Abnormal Wickham yeast or fermentation broth containing it 1.5;

[0089] Lactobacillus plantarum or fermentation broth 1 containing the same;

[0090] The preparation method of the composite microbial inoculum comprises the steps of inoculating yeast Wickham abnormality and Lactobacillus plantarum respectively in a medium containing carbon source, nitrogen source and inorganic salt after sterilization to carry out aeration culture to prepare a fermentation liquid, The fermentation broth is compounded in proportion or purified in proportion.

[0091] The above-mentioned preparation method comprises the following processing steps:

[0092] (1) Strain activation

[0093] The abnormal Wickham yeast and Lactobacillus plantarum are respectively inoculated on the corresponding sterile liquid medium,...

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Abstract

The invention relates to microbial strains and applications thereof, in particular to a composite microbial bacterial agent, its preparation and the application of ammonia and hydrogen sulfide in rapidly reducing farm environments. The invention provides a compound microbial agent containing abnormal Wickham yeast and Lactobacillus plantarum and a preparation method thereof. The microbial agent can quickly and effectively reduce ammonia and hydrogen sulfide in the environment of livestock and poultry farms , the content of harmful substances such as ammonia and hydrogen sulfide after using the composite microbial bacterial agent of the present invention meets the relevant requirements of industry standards, and has broad application prospects.

Description

technical field [0001] The invention relates to microbial strains and applications thereof, in particular to a compound microbial inoculum and its preparation and application in rapidly reducing ammonia and hydrogen sulfide in the environment of farms. Background technique [0002] Odor pollution is one of the seven major environmental hazards recognized in the world. As a large livestock and poultry breeding country, the odor emitted by farms has become one of the main sources of agricultural pollution in my country. The odor of farms not only affects the surrounding environment, but also directly affects livestock and breeding. staff health. Ammonia and hydrogen sulfide in the stench are soluble or easily soluble in water. These harmful gases are easily adsorbed on the mucous membranes of the respiratory system of livestock and even people. If animals are exposed to and live in a harmful gas environment for a long time, their immune system functions will be impaired. If it...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16B01D53/84B01D53/58B01D53/52C12R1/25C12R1/645
CPCC12N1/20C12N1/16B01D53/84B01D53/58B01D53/52B01D2251/95Y02A50/20
Inventor 王旭王占武胡栋朱德富彭杰丽张翠绵贾楠马佳
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI