Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Duplex breeding method for oilseed rape

A rapeseed, chromosome doubling technology, applied in botany equipment and methods, applications, plant gene improvement, etc., to achieve the effects of rich and diverse mutation forms, improved efficiency, and increased success rate

Active Publication Date: 2021-06-08
GUANGXI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Rapeseed oil extracted from rapeseed is one of the main edible oils in my country. It is rich in unsaturated fatty acids such as linoleic acid and vitamin E and other nutrients. It can be well absorbed by the body and has a good effect of softening blood vessels and delaying aging. It can also be used as industrial oil and other raw materials. Obviously, the existing rapeseed varieties are difficult to meet people's needs. Therefore, it is of great significance to provide a rapeseed with high yield, high oil yield, high disease resistance and a breeding method.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Duplex breeding method for oilseed rape

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Utilizing 40KHz ultrasonic-assisted mutagen to soak the rapeseed seeds of three strains of Chinese cabbage type rape, shepherd's purse type rape and Brassica napus for 12 hours, so that the chromosomes of the rapeseed species are doubled;

[0026] The mutagen consists of 0.05 parts by mass of ethylene sulfate, 0.1 part of aromatic oxyacetic acid glucose lipid, 0.05 part of nitrosoguanidine, 0.2 part of colchicine, 30 parts of rapeseed oil, fatty alcohol polyoxyethylene ether phosphoric acid 3 parts of ester sodium salt, 7 parts of fatty alcohol polysalt vinyl ether and 60 parts of ethanol are mixed;

[0027] (2) Spray the soaked seeds with a concentration of 1.0×10 5 After cfu / ml pathogenic bacteria liquid, plant different strains into the seed nursery, open pollination, select plants with light disease, multi-branching, short stalk, and high-oil traits, and select full-bodied rapeseed seeds from each plant , get the first-generation chromosome doubling female seri...

Embodiment 2

[0032] (3) Utilizing 60KHz ultrasonic-assisted mutagen to soak the rapeseeds of two strains of shepherd's purse and Brassica napus for 10 hours, so that the chromosomes of the rape varieties are doubled;

[0033] The mutagen consists of 0.06 parts by mass of ethylene sulfate, 0.2 parts of aromatic oxyacetic acid glucose lipid, 0.1 part of nitrosoguanidine, 0.1 part of colchicine, 20 parts of rapeseed oil, fatty alcohol polyoxyethylene ether phosphoric acid 4 parts of ester sodium salt, 6 parts of fatty alcohol polysalt vinyl ether and 60 parts of ethanol are mixed;

[0034] (4) Spray the soaked seeds with a concentration of 1.0×10 4 After cfu / ml pathogenic bacteria liquid, plant different strains into the seed nursery, open pollination, select plants with light disease, multi-branching, short stalk, and high-oil traits, and select full-bodied rapeseed seeds from each plant , get the first-generation chromosome doubling female series as the basic population;

[0035] Describe...

Embodiment 3

[0039] (5) Utilize 50KHz ultrasonic-assisted mutagen to soak the rape seeds of two strains of Chinese cabbage type rape and shepherd's purse type rape for 11 hours, so that the chromosomes of the rapeseed species are doubled;

[0040] The mutagen consists of 0.06 parts by mass of ethylene sulfate, 0.1 part of aromatic oxyacetic acid glucose lipid, 0.08 part of nitrosoguanidine, 0.15 parts of colchicine, 25 parts of rapeseed oil, fatty alcohol polyoxyethylene ether phosphoric acid 4 parts of ester sodium salt, 6 parts of fatty alcohol polysalt vinyl ether and 60 parts of ethanol are mixed;

[0041] (6) Spray the soaked seeds with a concentration of 6×10 4After cfu / ml pathogenic bacteria liquid, plant different strains into the seed nursery, open pollination, select plants with light disease, multi-branching, short stalk, and high-oil traits, and select full-bodied rapeseed seeds from each plant , get the first-generation chromosome doubling female series as the basic populatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of agricultural breeding technologies, and particularly discloses a duplex breeding method for oilseed rape. According to the method, different strains of rape seeds are soaked by a mutagenic agent consisting of ethylene sulfate, aryloxyacetic acid glucose ester, nitrosoguanidine, colchicine, rapeseed oil, fatty alcohol polyoxyethylene ether phosphate sodium salt, fatty alcohol polysalt vinyl ether and ethanol, and the chromosome of the rape varieties is doubled under the assistance of ultrasonic waves; then, the rape seeds infected and soaked by pathogenic bacteria causing pathogenicity to the oilseed rape are used, and the different strains of oilseed rape treated in such a way are interplanted to obtain a first-generation chromosome doubled basic group; and then, breeding for 6 to 8 comprehensive cycles is carried out to obtain an excellent oilseed rape seed line with stable characters, and the oilseed rape planted by the rape seed line is high in yield, high in oil yield, strong in disease resistance and low in plant morbidity.

Description

technical field [0001] The invention belongs to the technical field of agricultural breeding, in particular to a method for doubling breeding of rapeseed. Background technique [0002] The study found that due to the doubling of chromosomes, qualitative changes caused by quantitative changes, a series of plant characteristics have undergone great changes: its external morphological characteristics are significantly different from those of the original plant, often with huge tissues, such as: tall plants, hypertrophic And dark green leaves, larger or double flowers, larger fruits or seeds, enlarged and thicker stems or roots, etc.; chromosome doubled plants often contain more carbohydrates, proteins, and proteins than the original plants. Vitamins, chlorophyll, and certain useful chemicals such as alkaloids, sugars, and other medicinal ingredients and minerals; the disease resistance, drought resistance, cold resistance, and adaptability to adverse environments of chromosome-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H1/04
CPCA01H1/08A01H1/04
Inventor 杨欣欣王伟超莫颖颖吴严严严坚强
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com