Method for constructing zebra-fish melanin deposition model

A technology of melaninization and construction method, which is applied in the field of zebrafish melaninization model construction, can solve the problems of high laboratory requirements, long time consumption of chickens, large sample volume, etc., and achieve the effect of reduced experimental requirements, easy breeding, and small individuals

Inactive Publication Date: 2021-06-08
SUZHOU UNIV
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Problems solved by technology

Guinea pigs, mice, and chickens are time-consuming, costly, and h

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  • Method for constructing zebra-fish melanin deposition model

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Embodiment 1

[0046] A method for constructing a zebrafish melanosis model, comprising the following steps:

[0047] 1) Put the sexually mature zebrafish into the mating fish tank according to the ratio of male to female ratio = 1:2, insert a partition, and separate the male and female zebrafish; after overnight, turn on the light and change the water, take out the partition, and wait for 30-60 minutes collect fish eggs;

[0048] 2) The collected fish eggs are placed in a petri dish containing methylene blue, and the fertilized fish eggs are selected, divided into plates at a density of 100 pieces / plate, added with embryo culture medium, and incubated at a constant temperature of 28.5°C for 5 hours;

[0049] 3) After constant temperature incubation, fertilized eggs with excellent development were picked out and distributed in 6-well plates, randomly divided into a control group and three concentrations of sodium arsenite solution groups, and 3ml of fish culture was added to each well of the...

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Abstract

The invention discloses a method for constructing a zebra-fish melanin deposition model. The method comprises the following steps: putting sexually mature zebra-fish into a mating fish tank according to male and female, and separating the male and female zebra-fish by inserting a separator; turning on a lamp to change water overnight, taking out the separator, and collecting roes after 30-60 minutes; placing the roes in a culture dish containing methylene blue, selecting fertilized fish eggs to a separated dish and adding embryo culture solution into the fertilized fish eggs for constant-temperature incubation; selecting well-developed fertilized eggs, randomly dividing the fertilized eggs into a control group and a sodium arsenite solution group, and carrying out constant-temperature culture; and performing microscopic observation on the cultured zebra-fish, and obtaining the zebra-fish melanin deposition model if the melanin generation of the sodium arsenite solution group is increased in comparison with the control group. According to the method, the zebra-fish melanin generation increasing model can be quickly established in a short time after treatment of the sodium arsenite solution. The zebra-fish is short in growth cycle, easy to reproduce, large in egg laying amount, small in size and easy to breed, so that the experimental requirements can be greatly reduced, and a simple, efficient and low-cost biological model is provided for the mechanism research of arsenic-induced skin pigmentation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for constructing a zebrafish melanosis model. Background technique [0002] Arsenic is a naturally occurring environmental poison, and long-term exposure to arsenic can cause endemic arsenicosis, referred to as endemic arsenicosis. Ground arsenic disease is a biogeochemical disease, which is caused by the loss of skin pigmentation or / and excessive sedation caused by the long-term intake of excessive inorganic arsenic through drinking water, air or food by residents living in a specific geographical environment. , palmoplantar keratosis and cancerous systemic chronic poisoning. The symptoms of arsenicosis patients first appear on the skin, so skin lesions are regarded as an early sign of arsenic poisoning. At present, animal models of pigmentation have been widely used in the research of arsenic-induced skin hyperpigmentation. An ideal animal model for pig...

Claims

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Application Information

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IPC IPC(8): C12N5/073A01K67/027
CPCC12N5/0604A01K67/027A01K2227/40A01K2267/03C12N2500/05
Inventor 安艳魏园杰严鋭景楠王鲁娜杨乾磊
Owner SUZHOU UNIV
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