Beef tallow substitute fat as well as preparation method and application thereof in hotpot condiment
A technology to replace fat and butter, applied in the fields of application, edible oil/fat, food science, etc., to achieve the effects of stable ingredients, optimized physical and chemical properties, and improved flavor characteristics
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Embodiment 1
[0037] A kind of production technology of tallow substitute fat, comprises the following steps:
[0038] (1) According to parts by weight, take 50 parts of tallow and 50 parts of mid-melting point palm oil (melting point is 36.6 ° C), heat to melt, and mix evenly;
[0039] (2) Add immobilized lipase Novo 435 at a ratio of 30000U / g substrate to carry out transesterification reaction, the reaction temperature is 60°C, the reaction time is 6h, and the stirring rate is 800r / min;
[0040] (3) Filtrate after centrifugation at 5000r / min for 10min, take out the immobilized lipase, and stop the reaction;
[0041] (4) Add lipoxygenase at the ratio of 20000U / g substrate, the reaction temperature is 40°C, the stirring rate is 800r / min, and the reaction is 3h;
[0042] (5) Inactivate the enzyme at 100°C for 10 minutes;
[0043] (6) Centrifuge at 10,000r / min for 10min, remove residue and filter for 3 times, cool to room temperature, and obtain butter instead of fat.
Embodiment 2
[0045] A kind of production technology of tallow substitute fat, comprises the following steps:
[0046] (1) In parts by weight, weigh 20 parts of tallow, 60 parts of mid-melting point palm oil (36.6° C.) and 20 parts of palm stearin, heat to melt, and mix evenly;
[0047] (2) Adding immobilized lipase Lipozyme TL IM at a ratio of 30000U / g substrate for transesterification reaction, the reaction temperature is 55°C, the reaction time is 9h, and the stirring rate is 800r / min;
[0048] (3) Filtrate after centrifugation at 5000r / min for 10min, take out the immobilized lipase, and stop the reaction;
[0049] (4) Add lipoxygenase according to the ratio of 20000U / g substrate, the reaction temperature is 40°C, the stirring speed is 800r / min, and the reaction is 4h;
[0050] (5) Inactivate the enzyme at 100°C for 10 minutes;
[0051] (6) Centrifuge at 10,000r / min for 10min, remove residue and filter for 3 times, cool to room temperature, and obtain butter instead of fat.
Embodiment 3
[0053] A kind of production technology of tallow substitute fat, comprises the following steps:
[0054] (1) According to parts by weight, take 30 parts of tallow, 30 parts of palm olein and 40 parts of palm stearin, heat to melt, and mix evenly;
[0055] (2) Adding immobilized lipase Lipozyme TL IM at a ratio of 30000U / g substrate for transesterification reaction, the reaction temperature is 55°C, the reaction time is 9h, and the stirring rate is 800r / min;
[0056] (3) Filtrate after centrifugation at 5000r / min for 10min, take out the immobilized lipase, and stop the reaction;
[0057] (4) Add lipoxygenase according to the ratio of 20000U / g substrate, the reaction temperature is 40°C, the stirring speed is 800r / min, and the reaction is 4h;
[0058] (5) Inactivate the enzyme at 100°C for 10 minutes;
[0059] (6) Centrifuge at 10,000r / min for 10min, remove residue and filter for 3 times, cool to room temperature, and obtain butter instead of fat.
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