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High-heat-resistance stabilizer, microbial preparation and preparation methods and application of high-heat-resistance stabilizer and microbial preparation

A stabilizer and high heat-resistant technology, applied in the field of biotechnology and microbial products, can solve the problems of poor heat resistance, restricting the development of microbial preparations, inactivation, etc., and achieve the effect of simple ingredients, widening the scope of use, and improving the survival rate

Pending Publication Date: 2021-06-11
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When these microorganisms are added to feed additives as active ingredients, they need to go through the process of instantaneous high temperature, and the extremely poor heat resistance will quickly inactivate, which seriously restricts the development of microbial preparations in the field of animal feed additives. It is necessary to add appropriate heat-resistant protection agents to improve the thermal stability of microbial preparations

Method used

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  • High-heat-resistance stabilizer, microbial preparation and preparation methods and application of high-heat-resistance stabilizer and microbial preparation
  • High-heat-resistance stabilizer, microbial preparation and preparation methods and application of high-heat-resistance stabilizer and microbial preparation
  • High-heat-resistance stabilizer, microbial preparation and preparation methods and application of high-heat-resistance stabilizer and microbial preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The optimization of embodiment 1 heat-resistant stabilizer

[0030] Different heat-resistant stabilizers were prepared according to the compositions in Table 1, and there were 28 groups and 1 control group in total.

[0031] Table 1 The components of the heat-resistant stabilizer

[0032]

[0033]

[0034] (1) Dissolve the above components in water for injection, fully dissolve and heat-sterilize at 110°C for 15 minutes;

[0035] (2) Adjust the pH of the solution prepared in step (1) to 7.2 with sterile dilute hydrochloric acid and / or sodium hydroxide to obtain the heat-resistant stabilizer.

[0036] Coliphage (≥10 7 PFU / mL) were uniformly mixed with the above-mentioned heat-resistant stabilizer at a volume ratio of 1:1 to obtain a coliphage preparation with a heat-resistant protective agent.

[0037] The Escherichia coli phage preparation was stored at 60°C for 5 minutes at a high temperature, its potency was measured, and the protective effect of each protect...

Embodiment 2

[0042] The optimization of embodiment 2 heat-resistant stabilizer concentration

[0043] Select the heat-resistant stabilizer component finally obtained in Experimental Example 1, prepare the heat-resistant stabilizer according to the components shown in Table 3, the balance is deionized water, and set up a control group 1, and its sterilization method refers to Example 1. The preparation method of each group of coliphage preparations with heat-resistant stabilizers is the same as in Example 1.

[0044] Table 3 heat-resistant stabilizer component concentration

[0045] group Pullulan (%) Trehalose (%) Hyaluronic acid (%) Ethylenediaminetetraacetic acid(%) Magnesium chloride (%) 1 4 10 1.5 2 1.5 2 7 10 1.5 2 1.5 3 10 10 1.5 2 1.5 4 7 5 1.5 2 1.5 5 7 10 1.5 2 1.5 6 7 15 1.5 2 1.5 7 7 10 0.5 2 1.5 8 7 10 1 2 1.5 9 7 10 1.5 2 1.5 10 7 10 1.5 0.5 1.5 11 7 10 1.5 1 1...

Embodiment 3

[0050] Embodiment 3: the preparation of high heat-resistant stabilizer and phage preparation

[0051] High heat-resistant stabilizer, composed of the following components in mass percentage: 4% pullulan, 15% trehalose, 0.5% hyaluronic acid, 0.5% ethylenediaminetetraacetic acid, 1% magnesium chloride, and the balance is Water for Injection.

[0052] The specific preparation steps of high heat-resistant stabilizer are as follows:

[0053] (1) Weigh 4g of pullulan, 15g of trehalose, 0.5g of hyaluronic acid, 0.5g of ethylenediaminetetraacetic acid, and 1g of magnesium chloride according to the above mass ratio, dissolve them in 100mL of water for injection, fully dissolve and heat at 110°C Sterilize for 15 minutes;

[0054] (2) Adjust the pH of the solution prepared in step (1) to 7.2 with sterile dilute hydrochloric acid and / or sodium hydroxide to obtain the high heat-resistant stabilizer.

[0055] Preparation of phage preparations: Escherichia coli phage (≥10 7 PFU / mL) and t...

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Abstract

The invention discloses a high-heat-resistance stabilizer and a preparation method and application thereof. The high-heat-resistance stabilizer comprises the following components in percentage by weight: 4-10% of pullulan, 5-15% of trehalose, 0.5-1.5% of hyaluronic acid, 0.5-2% of ethylenediamine tetraacetic acid, 1-3% of magnesium chloride and the balance of water for injection. The invention further discloses a microbial preparation containing the high-heat-resistance stabilizer as well as a preparation method and application of the microbial preparation. The preparation process of the high-heat-resistance stabilizer is simple, safe and easy to implement, the survival rate of microorganisms at the temperature of 60-80 DEG C can be remarkably increased, and the development prospect of the microbial preparation as a green feed additive in the animal husbandry industry is greatly improved.

Description

technical field [0001] The invention belongs to the field of biotechnology and microbial products, and in particular relates to a high heat-resistant stabilizer, a microbial preparation and a preparation method and application thereof. Background technique [0002] In recent years, the presence of multidrug-resistant bacteria in animal products poses a serious threat to public health, especially in a part of the country where antibiotics are used unrestrictedly in animal husbandry. The overuse of antibiotics has caused the emergence of multidrug-resistant bacteria and the problem of drug residues is becoming more and more serious. Many countries have successively promulgated laws and regulations to prohibit the use of antibiotics. With the limited use of antibiotics, it is urgent to find new antibacterial agents that can replace antibiotics. [0003] In recent years, phages and probiotics have received more and more attention as effective feed additives and disease control ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/20C12N7/00A23K10/18C12R1/92C12R1/225
CPCC12N1/04C12N1/20C12N7/00A23K10/18C12N2795/10121C12N2795/10131
Inventor 左晓昕吕芳周明旭赵艳红卢宇邓碧华王军宁
Owner JIANGSU ACAD OF AGRI SCI