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Trichoderma harzianum, culture method, application and composite biological agent

A technology of biological preparations and inoculants, applied in the field of microorganisms, can solve the problems of canker spots on citrus leaves, branches and stems, economic losses, yield and quality decline, etc., and achieve the effects of improving quality, increasing yield, and achieving good control effects.

Active Publication Date: 2021-06-11
金禾佳农(北京)生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Citrus canker is a domestic and foreign quarantine bacterial disease caused by Xanthomonas axonopodispv.citri. , fruit, and branch canker spots, leading to weak growth of tree vigor, decline in yield and quality, and causing huge economic losses
[0003] At present, the prevention and control of citrus canker in China is mainly based on chemical control. As we all know, the long-term and large-scale use of chemical pesticides often leads to many problems such as environmental pollution, pesticide residues, and drug resistance of pathogenic bacteria that are difficult to solve.

Method used

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  • Trichoderma harzianum, culture method, application and composite biological agent
  • Trichoderma harzianum, culture method, application and composite biological agent
  • Trichoderma harzianum, culture method, application and composite biological agent

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Experimental program
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Effect test

Embodiment 1

[0034] The source and screening of embodiment 1 strain Bacillus subtilis

[0035] (1) Separation and purification of rhizobacteria

[0036] The soil samples were collected from the rhizosphere soil of Wogan in Sancai Village, Shuiyuan Town, Huanjiang Maonan Autonomous County, Huanjiang Maonan Autonomous County, Hechi City, Guangxi Zhuang Autonomous Region by using the "three-point sampling method", and were separated according to the conventional plate dilution method of soil microorganisms. Weigh about 10g of the rhizosphere soil sample, put it into a glass container filled with 100mL of sterile water and vibrate for 30 minutes, so that the soil particles in it are evenly dispersed, and after standing for 5 minutes, take the supernatant part of the soil suspension for 10 times gradient Diluted sequentially to 10 -3 、10 -4 、10 -5 , take 0.1mL of each dilution and apply it to a PDA plate (potato 200g, glucose 20g, H 2 O 1L, agar 20g, pH 7), all the plates were placed in a c...

Embodiment 2

[0039] The preparation method of embodiment 2 Trichoderma harzianum (Trichoderma harzianum) JNHC bacterial agent

[0040] (1) Inoculate the activated Trichoderma harzianum (Trichoderma harzianum) JNHC strain on the PDA liquid culture medium, under the condition of rotating speed of 100-140r / min, the cultivation temperature is 25-30°C, the cultivation time is 50-72h, and the preparation get seed liquid;

[0041](2) inoculating the seed solution prepared in step (1) into a liquid fermentation medium, and fermenting and culturing under the condition of 25-30° C. to obtain a fermentation liquid;

[0042] (3) Inoculate the fermented liquid prepared in step (2) in a solid fermentation medium at a ratio of 20%-30% by mass, and ferment 72% of the solid state under the conditions of 25-30°C and air humidity of 60%-90%. -96h, dry below 40°C until the moisture content is less than 10%, pulverize, and pack.

[0043] Also include the step of activating the Trichoderma harzianum (Trichode...

experiment example 1

[0054] Experimental example 1 Trichoderma harzianum (Trichoderma harzianum) JNHC bacterial agent applied to Citrus citrus

[0055] In this experiment, 2 treatments were set up, and each treatment was repeated 3 times. 15 citrus citrus plants were randomly selected in each repetition, and 6 plots were set up. Each plot was randomly arranged, with a total of 90 citrus citrus plants.

[0056] Treatment 1 (control group): apply water 50g / tree / time to Citrus citrus, once every 20 days.

[0057] Treatment 2 (experimental group): Trichoderma harzianum (Trichoderma harzianum) JNHC fungal agent was applied to the tangerines, the dosage of the Trichoderma harzianum fungal agent was 50 g / tree / time, and it was applied once every 20 days.

[0058] 1. Chlorophyll content in leaves of Wokan

[0059] 5 citrus oranges were randomly selected in each plot, and each plant was divided into upper, middle and lower parts. 5 leaves were randomly picked from each part, and the 3 Measure at different...

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Abstract

The invention discloses a Trichoderma harzianum JNHC strain as well as a culture method and application thereof, and belongs to the technical field of microorganisms. The Trichoderma harzianum JNHC strain provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is November 20, 2017, and the preservation number is CGMCC NO.14638. The Trichoderma harzianum JNHC provided by the invention has a good prevention effect on the citrus canker; the growth and production promoting effects on the citrus are good; and a Trichoderma harzianum JNHC microbial inoculum has high combining ability with other pesticides and chemical fertilizers, can protect plant root systems, and can improve the utilization rate of the fertilizers.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Trichoderma harzianum JNHC strain, a culture method, an application and a composite biological preparation. Background technique [0002] Citrus canker is a domestic and foreign quarantine bacterial disease caused by Xanthomonas axonopodispv.citri. , fruit, and canker spots on branches lead to weak growth of tree vigor, decline in yield and quality, and cause huge economic losses. [0003] At present, the prevention and control of citrus canker in China is mainly based on chemical control. As we all know, the long-term and large-scale use of chemical pesticides often leads to many problems such as environmental pollution, pesticide residues, and drug resistance of pathogenic bacteria, which are difficult to solve. The use of antibacterial active substances produced by plant endophytes for biological control of plant diseases has the advantages of high efficiency, low to...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P1/00A01P21/00C05F11/08C05G3/80C12R1/885
CPCC12N1/14A01N63/38C05F11/08C05G3/80
Inventor 张丽霞
Owner 金禾佳农(北京)生物技术有限公司
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