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Bifidobacterium bifidum for relieving constipation and its fermented food and probiotic preparation

A technology of bifidobacteria bifidum and fermented food, applied in the field of microorganisms, can solve the problems of single function of bifidobacteria, partial functions are not prominent, etc., and achieve the effect of reducing the time of the first black stool, increasing the effect, and improving the water content of the stool

Active Publication Date: 2022-07-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on bifidobacteria for relieving constipation will have a huge impact on food science, microbiology, and preventive medicine. Due to the single function of bifidobacteria that has the effect of relieving constipation in the prior art and some functions are relatively small Not outstanding, therefore, research on the effect of bifidobacteria on constipation relief is needed

Method used

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  • Bifidobacterium bifidum for relieving constipation and its fermented food and probiotic preparation
  • Bifidobacterium bifidum for relieving constipation and its fermented food and probiotic preparation
  • Bifidobacterium bifidum for relieving constipation and its fermented food and probiotic preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1: Acquisition of Bifidobacterium bifidum CCFM1167

[0104] Specific steps are as follows:

[0105] 1. Isolation and screening of Bifidobacterium strains:

[0106] (1) Use a disposable sterile toilet to collect the feces of a woman, put the fecal samples in a liquid medium containing MRS + mass percent (0.05%-0.1%) cysteine ​​containing fructooligosaccharides, in an anaerobic incubator (N2:CO2:H2=80:10:10) for 12h;

[0107] (2) The fecal sample was diluted with sterile physiological saline and then applied to MRS+ mass percentage (0.05%-0.1%) L- On the solid plate of cysteine ​​hydrochloride, cultivate for 24-48h;

[0108] (3) choose the single colony that accords with the basic form of bifidobacteria to carry out streak purification on the plate, and screen and isolate the selected strain of bifidobacteria;

[0109] (4) The above single colony was cultured in liquid MRS+mass percentage (0.05%-0.1%) cysteine ​​medium for 24 hours, and then Gram-stained, and ...

Embodiment 2

[0128] Example 2: Relief of loperamide-induced constipation-related symptoms by Bifidobacterium bifidum CCFM1167

[0129] Specific steps are as follows:

[0130] (1) Preparation of Bifidobacterium bifidum CCFM1167 bacterial suspension

[0131] Bifidobacterium bifidum CCFM1167 strain was taken out from the refrigerator at -80°C, streaked in MRS solid medium, cultivated at 37°C for 48h, picked a single colony in MRS liquid medium, and cultivated at 37°C for 24h to prepare seeds liquid;

[0132] The prepared seed liquid was inoculated into a new MRS liquid medium with an inoculum of 2% (v / v), cultivated at 37 ° C for 24 h, and cultured for another generation in the same way to prepare Bifidobacterium bifidum CCFM1167 fermentation liquid;

[0133] Then, the prepared Bifidobacterium bifidum CCFM1167 fermentation broth was centrifuged at 6000 r / min and 4°C for 5 min, and then resuspended with skimmed milk with a mass-to-volume ratio of 10% to obtain a bacterial suspension for ani...

Embodiment 3

[0145] Example 3: Bifidobacterium bifidum CCFM1167 reduces the content of somatostatin (SS) in serum of constipated mice

[0146] Specific steps are as follows:

[0147] (1) The grouping, modeling and treatment methods of C57BL / 6J mice were the same as those in Example 2.

[0148] (2) After the mice were sacrificed on the 40th day, the collected blood of the mice was allowed to stand for 2 hours, and the serum was obtained after centrifugation at 3000 × g for 15 minutes. The somatostatin (SS) detection kit was used. The concentration of SS in serum was calculated.

[0149] Studies have shown that somatostatin can inhibit gastrointestinal motility and secretion of digestive tract hormones.

[0150] The experimental results are as figure 2 As shown, the model group (the content of somatostatin SS was 6.410ng / L) had an upward trend compared with the control group (P=0.0330), while the Bifidobacterium bifidum CCFM1167 group (the content of somatostatin SS was 5.266ng / L) ) Com...

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Abstract

The invention discloses bifidobacteria bifidum for relieving constipation and a fermented food and probiotic preparation prepared therefrom, belonging to the technical field of microorganisms. The Bifidobacterium bifidum CCFM1167 provided by the invention can effectively relieve the first black stool time, intestinal propulsion rate and fecal water content of constipated mice, and can obviously improve the apparent pathological indexes of constipation; In the detection of Bifidobacterium bifidum, Bifidobacterium bifidum can also affect the content of gastrointestinal active peptides, thereby affecting intestinal peristalsis; Bifidobacterium bifidum can also effectively relieve the pathological damage of colon tissue, thereby effectively relieving constipation symptoms, and has Compared with Fidobacteria GDMCC NO: 60941, it has a more significant effect on the MTL content, and then has a more obvious effect on the increase of the small intestinal propulsion rate, which is more suitable for constipation patients with weak small intestinal propulsion.

Description

technical field [0001] The present invention relates to bifidobacteria bifidum for relieving constipation, and fermented food and probiotic preparations prepared therefrom, belonging to the field of microorganisms. Background technique [0002] According to incomplete statistics, the prevalence of constipation in the world is about 20.0%, and the prevalence of constipation in Chinese adults is 7.0%-20.3%. Meanwhile, the prevalence in the Asia-Pacific region is 8.75%, and the prevalence in Western countries is as high as 27%; the prevalence in children and adolescents is 0.7-29.6%, and the prevalence in the elderly is 15-50%. It can be seen that constipation is not only a disease with a high prevalence rate, but also has an extremely wide range. [0003] With the change of diet structure and the change of life rhythm, the number of patients with constipation is increasing. Although constipation does not directly threaten people's life safety, the harm caused by this disease ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123A23L11/65A23L11/50A23L19/00A23L17/60A23L33/135A61K35/745A61P1/10C12R1/01
CPCC12N1/20A23C9/1234A23C11/106A23L19/00A23L17/60A23L17/65A23L33/135A61K35/745A61P1/10A23V2002/00A23V2400/517A23V2200/3204
Inventor 王刚王琳琳柴茂赵建新陈卫张灏
Owner JIANGNAN UNIV
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