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Active oligopeptide hydrogel material as well as preparation method and application thereof

A hydrogel and oligopeptide technology, applied in the field of biomedical materials, can solve the problems of difficulty in extraction and synthesis, long peptide sequence and high cost, and achieve the effects of easy extraction and synthesis, low cost and improved mechanical strength

Pending Publication Date: 2021-06-15
中科院过程工程研究所南京绿色制造产业创新研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the peptide sequences involved in the above-mentioned peptide gel materials are relatively long, making extraction and synthesis difficult and costly

Method used

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  • Active oligopeptide hydrogel material as well as preparation method and application thereof
  • Active oligopeptide hydrogel material as well as preparation method and application thereof
  • Active oligopeptide hydrogel material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] This embodiment provides a kind of active oligopeptide hydrogel material, the preparation method is as follows:

[0050] Weigh 2.0 mmol of palmitoyl hexapeptide-9, disperse it in 100 mL of phosphate-buffered saline (0.1 M, pH=7.4), add 0.1 mmol of zinc chloride, mix well, and heat up to 80°C to obtain a uniform Disperse the solution; place it at 4°C, let it stand for aging for 12 hours, and finally obtain the hydrogel of palmitoyl hexapeptide-9.

[0051] Under the above process conditions, the obtained hydrogel physical picture is as follows: figure 1 In a, the appearance of the hydrogel is uniform and transparent, and it is in a stagnant state after inversion. After pushing and pulling before injection (simulating smearing effect), it can be transformed into a sol state, such as figure 1 Shown in b.

Embodiment 2

[0053] This embodiment provides a kind of active oligopeptide hydrogel material, the preparation method is as follows:

[0054] Weigh 0.8mmol of palmitoyl tripeptide-5, disperse it in 100mL of tris(hydroxymethyl)aminomethane aqueous solution (0.01M), add 0.8mmol of silver nitrate, mix well, and heat up to 60°C to obtain a uniform dispersion solution ; After 0.5 hours of heat preservation, the temperature was slowly lowered (the cooling rate was 5°C / h), left to age for 8 hours, and finally the hydrogel of palmitoyl tripeptide-1 was obtained.

[0055] Under the above process conditions, the obtained hydrogel physical picture is as follows: figure 1 As shown in c, the hydrogel has a uniform appearance and is transparent, and it is in a stagnant state after inversion.

Embodiment 3

[0057] This embodiment provides a kind of active oligopeptide hydrogel material, the preparation method is as follows:

[0058] Weigh 0.8mmol of palmitoyl pentapeptide-4, disperse it in 100mL of pH=7.4 phosphate buffered saline, add 0.8mmol of cobalt chloride, mix evenly, raise the temperature to 60°C to obtain a uniform dispersion solution; place in 4 ℃, standing and aging for 12 hours, and finally a hydrogel of palmitoyl pentapeptide-4 was obtained.

[0059] Under the above process conditions, the obtained hydrogel physical picture is as follows: figure 1 As shown in d, the hydrogel is uniform in appearance, transparent, and in a stagnant state after inversion.

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Abstract

The invention relates to an active oligopeptide hydrogel material as well as a preparation method and application thereof. The active oligopeptide hydrogel material comprises active oligopeptide and metal ions complexed with the active oligopeptide, the active oligopeptide comprises any one or a combination of at least two of palmitoyl oligopeptide, a palmitoyl oligopeptide derivative, myristoyl oligopeptide and a myristoyl oligopeptide derivative. The active oligopeptide in the active oligopeptide hydrogel material is relatively short in peptide fragment sequence, easy to extract and synthesize and low in cost, and has a specific active function; and the metal ions widely exist in living bodies and play a crucial role in participating in important physiological activities such as various chemical reactions and signal transduction in a life system. The characteristics of the metal ions and the active oligopeptide are well combined, the mechanical strength of the material is improved, synergistic interaction is further achieved, and an active biological dressing material which is absorbable, free of irritation, high in safety, convenient to use and excellent in tissue adhesion effect is obtained.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and specifically relates to an active oligopeptide hydrogel material and its preparation method and application, in particular to an absorbable, non-irritating, high safety, convenient to use, and tissue-adhesive effect Excellent active oligopeptide hydrogel material and its preparation method and application. Background technique [0002] Hydrogel, a water-based gel that enhances absorption, is commonly used for transdermal and transmucosal drug delivery. Peptide-based hydrogel materials are widely used in tissue engineering, drug delivery, and cell culture due to their good biocompatibility, reversibility, and degradability. Currently, the types of peptides that can form peptidyl hydrogel materials include ion-complementary, peptide amphiphile, and β-hairpin, etc. For example: U.S. Patent US5670483A discloses an ion-complementary peptide (EAK16) found in yeast protein, which can ...

Claims

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Application Information

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IPC IPC(8): A61K47/18A61K47/42A61K47/02A61P17/02B01J13/00
CPCA61K47/18A61K47/42A61K47/02A61P17/02B01J13/0065
Inventor 闫学海沈桂芝邢蕊蕊袁成前
Owner 中科院过程工程研究所南京绿色制造产业创新研究院
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