Pea polypeptides and preparation method thereof
A pea polypeptide and a technology for preparation steps, applied in the field of pea polypeptide and its preparation, can solve problems such as unfavorable industrial production, complicated processing process, etc., and achieve the advantages of reducing standing and centrifugation time, accelerating precipitate formation, and facilitating nanofiltration desalination. Effect
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Embodiment 1
[0026] S100. Mix 100 grams of pea protein powder with a protein content of 72% and 2000 grams of pure water; use a mixed acid solution to adjust the pH to 3.5, and soak at 42°C for 4 hours, wherein the mixed acid solution consists of 0.2 mol of lactic acid and 0.03 mol of acetic acid. mol and malic acid 0.02mol;
[0027] S200, add 3.350 acid protease 380,000u, carry out the first hydrolysis at 52°C, and the hydrolysis time is 120min;
[0028] S300, the first hydrolyzate was inactivated at 85°C for 8 minutes, and the pH of the hydrolyzate was adjusted to 7.3 with a mixed calcium suspension; add 320,000 u of Amano NY50C neutral protease, and perform the second hydrolysis at 45°C, hydrolysis The time is 85 minutes; wherein, the mixed calcium suspension is a suspension prepared from 0.3 mol of calcium oxide, 0.05 mol of calcium sulfate and pure water.
[0029] S400. The second hydrolyzate was boiled at 100°C to inactivate the enzyme for 8 minutes. After standing still for 20 minu...
Embodiment 2
[0038] S100. Take 100 grams of pea protein powder with a protein content of 80% and mix it with 2200 grams of pure water; adjust the pH to 3.0 with a mixed acid solution and soak at 45°C for 3 hours, wherein the mixed acid solution consists of 0.2 mol of lactic acid and 0.04 mol of acetic acid mol prepared;
[0039] S200, add 3.350 acid protease 350,000u, carry out the first hydrolysis at 50°C, and the hydrolysis time is 100min;
[0040] S300, the first hydrolyzate was inactivated at 90°C for 5 minutes, and the pH value of the hydrolyzate was adjusted to 6.8 with the mixed calcium suspension; 400,000 u of NY50C protease was added, and hydrolyzed at 50°C for 60 minutes, wherein the mixed calcium suspension was Suspension prepared from 0.2mol calcium hydroxide, 0.03mol calcium sulfate and pure water;
[0041] S400, the second hydrolyzate was boiled at 100°C to inactivate the enzyme for 8 minutes; after standing still for 40 minutes, centrifuge at 8000rpm to remove the precipita...
Embodiment 3
[0044] S100. Take 100 grams of pea protein powder with a protein content of 80% and mix it with 2200 grams of pure water; adjust the pH to 3.2 with a mixed acid solution, soak at 42°C for 4 hours, wherein the mixed acid solution consists of 0.2 mol of lactic acid and 0.02 mol of acetic acid mol and malic acid 0.04mol;
[0045] S200, adding 400,000 u of 3.350 acidic protease, and performing the first hydrolysis at 55°C for 80 minutes;
[0046] S300, the first hydrolyzate was inactivated at 90°C for 8 minutes, and the pH value of the hydrolyzate was adjusted to 7.0 with the mixed calcium suspension; 450,000 u of NY50C protease was added, and hydrolyzed at 52°C for 70 minutes, wherein the mixed calcium suspension was prepared by Suspension prepared from 0.2mol calcium oxide, 0.03mol calcium sulfate and pure water;
[0047] S400, the second hydrolyzate was boiled at 100°C to inactivate the enzyme for 10min; after standing for 20min, centrifuge at 6000rpm to remove the precipitate...
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