Mutant for improving gene expression quantity of purine operon pur and construction method and application thereof

A technology of gene expression quantity and construction method, applied in the directions of microorganism-based methods, biochemical equipment and methods, resistance to vector-borne diseases, etc., to achieve the effect of increasing yield and improving gene expression level
CN112980756AActive Publication Date: 2021-06-18QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Authority / Receiving Office
CN · China
Current Assignee / Owner
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Publication Date
2021-06-18

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Abstract

The invention discloses a mutant for improving the gene expression quantity of a purine operon pur and a construction method and application thereof, and belongs to the technical field of gene engineering. Aiming at the problems of transcription inhibition of a purine operon pur and difficulty in accumulation of a purine compound in a purine de novo synthesis route, the mutant for improving the gene expression quantity of the purine operon pur is provided, and the mutant is constructed by knocking out DNA in escherichia coli and combining with a transcription two-component regulatory factor arcA (Genbank accession number is 948874). The mutant is further used as a starting strain, a mutated PRPP synthetase gene prs and a mutated PRPP transamidase gene purF are subjected to overexpression, and a recombinant strain with high yield of hypoxanthine is constructed. According to the mutant, regulation of the regulatory factor arcA in gene expression of the purine operon pur and application of the regulatory factor arcA in purine biosynthesis are realized for the first time.
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Description

technical field

[0001] The invention relates to a mutant for increasing the expression of purine operon pur gene, its construction method and application, and belongs to the technical field of genetic engineering. Background technique

[0002] Purine compounds are important life-active substances in cells, and play important roles in the synthesis and transmission of genetic material, signal transduction, and energy supply in cells. In addition, purine compounds can be used as precursors to synthesize a variety of drugs, and are also widely used in the diagnosis and treatment of diseases and life science research. For example, the early mercaptopurine drugs, and the late fludarabine, clorabine and other purine nucleoside drugs play an important role in cancer treatment.

[0003] The de novo synthesis route of purine has long been proven. The de novo synthesis of purine mainly uses glutamine and 5-phosphoribosyl pyrophosphate (PRPP) as precursors. After 10 steps of reaction,...

Claims

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