Synchronous detection method for organophosphate and metabolite in poultry egg products and application

An organic phosphate and synchronous detection technology, which is applied in measuring devices, computer-aided medical procedures, health index calculations, etc., can solve problems such as poor repeatability, large manpower consumption, and long analysis and detection time, and achieve high accuracy and quantity Multiple, high-sensitivity effects

Inactive Publication Date: 2021-06-18
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Using the method provided by it, the two types of pollutants are pre-treated and analyzed by instruments respectively, and the time spent is 4 hours and 8 hours respectively, accumulatively 12 hours; and the results detected by the respective instruments must be recorded, counted, analyzed, and exist. Long detection time, high cost of manpower, machine time and consumables, etc.
[0005] At the same time, there is currently insufficient research on the exposure characteristics of organophosphates and metabolites in poultry and egg products in the human population, and there is a lack of scientific and effective information analysis processes, analysis models and analysis tools. The data obtained through testing can only be obtained through manual First, it requires a lot of manpower to conduct research and judgment. Second, the results of research and judgment are related to the professional level of the analysts. The repeatability of the analysis method and process is poor, and it cannot guarantee the objectivity and stability of the research and judgment results of each batch. , thus affecting the objectivity and accuracy of health risk assessment

Method used

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  • Synchronous detection method for organophosphate and metabolite in poultry egg products and application
  • Synchronous detection method for organophosphate and metabolite in poultry egg products and application
  • Synchronous detection method for organophosphate and metabolite in poultry egg products and application

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Experimental program
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Embodiment

[0081] See Figure 1 to Figure 5 , the synchronous detection method of organophosphate and metabolite in poultry and egg product provided by the present invention, comprises the following steps:

[0082] 1) Construct a mathematical model for risk assessment: a risk assessment model for human exposure to ingestion of organophosphate parent compounds and their metabolites;

[0083] 2) Determine the components to be tested and the samples to be tested: According to the parameters involved in the mathematical model of health exposure risk assessment, select 19 specific substances in the organophosphate parent compounds (OPEs) and their metabolites (mOPEs) in poultry and egg products As the component to be tested, the sample to be tested shall be drawn in accordance with the requirements of the Health Exposure Risk Assessment Specification;

[0084] 3) Sample pretreatment: perform pretreatment on the sample, extract the supernatant from the unit sample and internal standard by sol...

specific Embodiment 1

[0144] The method for synchronous detection of organophosphates and metabolites in poultry and egg products provided by the present invention is basically the same as that of the foregoing embodiments, the difference being that the synchronous detection of organophosphates and metabolites specifically includes the following step:

[0145]1) Sample pretreatment: extract the supernatant from the unit poultry egg product sample and the internal standard by solid-liquid extraction, and purify the supernatant with a solid-phase extraction column to obtain the sample extract to be tested;

[0146] 1-1) Crush the unit egg product sample, add acetonitrile and toluene together with the internal standard for ultrasonic extraction, and separate the supernatant to obtain the extract; for every two grams of biological samples, add 20 μL, 1 μg / mL d27-TBOEP, d15-TPHP, d27-TBP, d18-TCPP, d12-TCEP, d8-BBOEP, d4-BBOEHEP, d10-DPHP, d8-BCEP and d10-BDCPP mixed internal standard; in order to impro...

specific Embodiment 2

[0194] The simultaneous detection method of organophosphates and metabolites in poultry and egg products provided in this example is basically the same as the previous example, the difference is that the sample to be tested is a suspected low-concentration exposed egg, which specifically includes the following steps :

[0195] 1) Sample pretreatment: extract the supernatant from the unit poultry egg product sample and the internal standard by solid-liquid extraction, and purify the supernatant with a solid-phase extraction column to obtain the sample extract to be tested;

[0196] Step 1) the unit sample and internal standard sampling solid-liquid extraction method and solid phase extraction method to obtain the detection sample extract;

[0197] 1-1) Accurately weigh 2g of low-concentration exposed egg samples and crush them into a 15mL glass centrifuge tube, and mix with 20ng, d27-TBOEP, d15-TPHP, d27-TBP, d18-TCPP, d12-TCEP, d8-BBOEP, d4 -BBOEHEP, d10-DPHP, d8-BCEP and d10...

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Abstract

The invention discloses a synchronous detection method for organophosphate and metabolites in poultry egg products and application. The method comprises the following steps: 1) constructing a risk assessment mathematical model; 2) determining components to be detected and a sample to be detected; 3) pre-treating the sample; 4) sample detecting; 5) obtaining detection data by an internal standard method; and 6) performing health exposure risk assessment. The invention also discloses application of detection data obtained by the detection method to organophosphorus ester and metabolite crowd feeding health exposure risk assessment analysis, including daily exposure quantity analysis, pollution source analysis, exposure sensitive crowd analysis, marker safety limit value analysis, risk analysis and grading, to organophosphorus ester and metabolite crowd feeding health exposure risk assessment analysis. According to the method, a risk assessment mathematical model is constructed, 19 specific substances in OPEs and mOPEs are selected as components to be detected, and synchronous detection is performed by adopting the ultra-high performance liquid chromatography-tandem mass spectrometry, so that the contents of the 19 specific substances can be obtained at the same time, and detection data and risk analysis results can be rapidly and efficiently obtained.

Description

technical field [0001] The invention relates to the technical field of organophosphate detection and health risk assessment, in particular to a simultaneous detection method and application of organophosphate and metabolites in poultry and egg products. Background technique [0002] Organophosphate ester compounds (Organophosphate esters, OPEs) and their metabolites (mOPEs) are mainly used as flame retardants and plasticizers, widely used in electronic products, plastic products, textiles and building materials, etc., in various environmental media and biological widely detected in vivo. OPEs, as a class of endocrine disruptors, have been proven to have a variety of biological toxicity, and can enter the human body through dietary exposure, causing potential health risks, thus attracting people's attention. However, studies in recent years have shown that OPEs are rapidly metabolized into mOPEs after entering the organism, and the toxicity of some mOPEs is equivalent to or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/86G16H20/60G16H50/30
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/8696G01N2030/062G01N2030/324G16H20/60G16H50/30
Inventor 于云江李宗睿朱钰李良忠向明灯陈希超党垚
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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