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Content determination method of Jingulian preparation

A determination method, the technology of the golden bone lotus, applied in the field of medicine, can solve the problems that cannot fully reflect the internal quality of the product

Pending Publication Date: 2021-06-18
GUIZHOU YIBAI PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although 200910312147.3 The detection method of the capsule preparation for the treatment of joint swelling and pain and the Jingulian capsule national drug standard WS-10093 (ZD-0093)-2002-2012Z all disclose the content assay method of Jingulian capsule, but only in its assay method The "fumaric acid" component in Chinese peach leaves was determined. As a compound preparation, Jingulian can only use a single component as a quantitative index to fully reflect the internal quality of the product. How to use simple and effective methods to effectively control product quality has become a problem. top priority

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Determination method of Jingulian capsule

[0061] 【Content Determination】Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2020 Edition Sibu General Rules 0512).

[0062] time (minutes) Mobile phase A (%) Mobile phase B (%) 0～40 5→20 95→80 40～50 20→25 80→75 50～55 25→35 75→65

[0063] Preparation of Reference Substance Solution Take an appropriate amount of reference substance fumaric acid, gallic acid, protocatechuic acid, salidroside, chlorogenic acid, vanillic acid, epicatechin, liriodendron, and albinoside A. Weigh it, add 50% methanol to dissolve it, and make each 1ml contain fumaric acid, salidroside, albinoside A, epicatechin, vanillic acid 40μg, contain chlorogenic acid, tulipin 80μg, and contain original A mixed solution of 60 μg of catechin and 20 μg of gallic acid is obtained.

[0064] Preparation of the test solution Take 1.0 g of the content of Jingulian capsule, accurately weigh it...

Embodiment 2

[0068] Determination method of Jingulian capsule

[0069] 【Content Determination】Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2020 Edition Sibu General Rules 0512).

[0070] time (minutes) Mobile phase A (%) Mobile phase B (%) 0～40 5→20 95→80 40～50 20→25 80→75 50～55 25→35 75→65

[0071] Preparation of Reference Substance Solution Take an appropriate amount of reference substance fumaric acid, protocatechuic acid, salidroside, chlorogenic acid, tulipin, and albinoside A, accurately weighed, and dissolve in 50% methanol to prepare each 1ml of a mixed solution containing 40 μg of fumaric acid, salidroside, and albinoside A, 80 μg of chlorogenic acid and turioside, and 60 μg of protocatechuic acid.

[0072] Preparation of the test solution Take 1.0 g of the contents of Jingulian capsules, accurately weighed, place in a stoppered Erlenmeyer flask, accurately add 25 ml of 50% methanol, seal the plug, wei...

Embodiment 3

[0075] Determination method of Jingulian tablets

[0076] 【Content Determination】Determination according to high performance liquid chromatography (Chinese Pharmacopoeia 2020 Edition Sibu General Rules 0512).

[0077] time (minutes) Mobile phase A (%) Mobile phase B (%) 0～40 5→20 95→80 40～50 20→25 80→75 50～55 25→35 75→65

[0078] Preparation of Reference Substance Solution: Take an appropriate amount of reference substance fumaric acid, salidroside, liriodendron, and albinoside A, accurately weighed, add 50% methanol to dissolve, and make each 1ml contain fumaric acid, rhodiola A mixed solution of 40 μg of albinoside A and 80 μg of lirioside is obtained.

[0079] Preparation of the test solution Take Jingulian tablets, grind finely, take 1.0g, accurately weigh, place in a stoppered Erlenmeyer flask, accurately add 25 ml of 50% methanol, seal tightly, weigh, and ultrasonically treat for 30 minutes (300 W, 40 kHz), cooled, supplemented with 5...

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Abstract

The invention provides a method for determining the content of a Jingulian preparation, and belongs to the technical field of medicines. The Jingulian preparation is prepared from psammosilene tunicoides, schefflera kwangsiensis, gaultheria yunnanensis, alangium platanifolium and sargentgloryvine stem. The content determination method comprises the step of determining the content of nine components such as fumaric acid, gallic acid, protocatechuic acid, salidroside, chlorogenic acid, vanillic acid, epicatechin, liriodendron glycoside and gaultheroside A in the product under the same chromatographic condition by adopting a high performance liquid chromatography. Compared with the prior art, the content determination of gallic acid, protocatechuic acid, salidroside, chlorogenic acid, vanillic acid, epicatechin, liriodendron glycoside and gaultheroside A is added, the requirement for product quality detection is improved, and medicine quality and clinical efficacy are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for determining the content of a japonicus preparation. Background technique [0002] Jingulian Capsule is derived from the classic prescription of Miao medicine, which is composed of Touguxiang, Hantao Leaf, Daxueteng, Star Anise Maple and Jintiesuo five herbs. It has the effects of expelling wind and dampness, reducing swelling and relieving pain, and is used for joint swelling and pain caused by rheumatic numbness, flexion, etc. It is widely used clinically for acute soft tissue injuries and osteoarthritis and other diseases. Although 200910312147.3 the detection method of the capsule preparation for the treatment of joint swelling and pain and Jingulian Capsule National Drug Standard WS-10093 (ZD-0093)-2002-2012Z all disclose the content assay method of Jingulian Capsules, but only in its assay method Determination of the "fumaric acid" component in Ch...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 窦啟玲黄勇浦健李勇军郑林谭丹
Owner GUIZHOU YIBAI PHARMA CO LTD