Agrocybe cylindracea cultivation method using camellia fruit tree stem raw material as culture medium

[0030] Example 1

[0031] The present embodiment provides a method for cultivating Camellia sinensis using the Camellia fruit stem raw material as a culture medium: comprising the following steps:

[0032] 1) Preparation of culture medium: the raw materials of the culture medium and their mass fractions are: 35-45% of camellia stalk, 25-35% of sawdust, 8-12% of wheat husk, 8-12% of corn flour and 1-3% of quartz. %, after the raw materials of the culture medium are evenly stirred, then according to the ratio of the material to water ratio of 1:1.1 to 1.13, add water and stir to obtain the culture medium; wherein, the preparation method of the camellia fruit stem is: After being pulverized by a crusher, the particles of the camellia stem are washed and then dried, and then the particles of the camellia stem are sieved and filtered to obtain the camellia stem particles with a particle size of 0.2-0.8 cm. In the present embodiment, the particle size of the camellia fruit stem is 0.2cm, and the camellia fruit stem raw material selected for the medium is rich in nutrients and has high content of protein and fat, so that the stem of the camellia mushroom develops more sturdy, thereby improving the output of the camellia mushroom .

[0033] Further, the raw materials of the culture medium and their mass fractions are: 42% of camellia fruit stems, 32% of sawdust, 14% of wheat husks, 10% of corn flour and 2% of quartz. Then add water in a ratio of 1:1.1 to obtain a culture medium.

[0034] Further, the wood chips are wood chips that have been sun-aged for 30 to 40 days, and the length of the wood chips is 0.1 to 0.7 cm. In this embodiment, more preferably, the wood chips are wood chips that have been sun-aged for 30 days, and the length of the wood chips is 0.1 cm. The wood chips after sun aging treatment are easy to decompose polysaccharides and monosaccharides. Sugars can provide carbon source for tea tree mushroom strains and are easily absorbed by tea tree mushrooms, thereby improving the edible taste of tea tree mushrooms and making them more sweet and refreshing. The particles of the raw materials used in the medium are smaller, so that the mixing of the raw materials is more sufficient, which is convenient for the root system of the tea tree mushroom to absorb, thereby shortening the growth cycle of the tea tree mushroom and increasing the yield.

[0035] Further, the water in the culture medium is condensed distilled water boiled at high temperature.

[0036] Further, the pH value of the culture medium is 5-6. In this example, the pH value of the culture medium was 5. A slightly acidic medium can be more conducive to the growth and reproduction of the tea tree mushroom, thereby increasing the yield of the tea tree mushroom.

[0037] 2) Sterilization treatment: After the culture medium is packaged with a bacterial bag, the bacterial bag is put into a sterilization room for sterilization, the sterilization temperature is 100-120°C, and the sterilization time is 9-12 hours. In this embodiment, it is more preferable that the sterilization temperature is 120° C., and the sterilization time is 9 hours.

[0038] Further, the bacteria bag is a polyethylene plastic bag.

[0039] 3) Tea tree mushroom inoculation: after the sterilization is completed, the bacteria bag is naturally cooled to normal temperature, placed in an inoculation box, an inoculation hole is opened on the bacteria bag, and the tea tree mushroom strain is put into the inoculation hole for aseptic inoculation.

[0040] 4) Mycelium culture: After the inoculation is completed, place the fungus bag in the growth chamber for fungus culture until the mycelium is filled with the fungus bag. During the process of mycelium cultivation, the temperature of the fungus chamber is controlled within 21-27°C. , keep the room ventilated and dry. In this embodiment, it is preferable that the temperature of the germ-generating chamber is controlled at 21°C.

[0041]5) Fruiting management: after the mushroom bag is covered with mycelium, the bag mouth of the mushroom bag is opened and placed in a greenhouse for fruiting management. The fruiting management includes the following steps:

[0042] a: Open the fungus bag and remove the old fungus and hyphae on the surface of the hyphae.

[0043] b: Straighten the bag mouth of the bacteria bag, and cover the bag mouth with newspaper, and spray the newspaper three times a day to raise the relative humidity of the air in the bacteria bag to 80-90%. Scattering light source, the light intensity of the scattering light source is 1200-1800Lux, the scattering light source is located above the bacteria bag, and the distance between the scattering light source and the bacteria bag is 80-130cm, and the scattering light source is turned off at night to keep the shed in a dark state. The temperature in the greenhouse is controlled between 20 and 26 °C, and at night, the temperature in the greenhouse is controlled between 12 and 18 °C, so that the temperature difference between daytime and night in the greenhouse reaches 6 to 8 °C, and the temperature difference lasts for 8 to 12 days. In this embodiment, it is preferred that the relative humidity of the air in the bacterial bag is increased to 80%, the light intensity of the scattered light source is 1200Lux, the distance between the scattered light source and the bacterial bag is 80cm, and the temperature in the greenhouse is controlled at 20°C during the day. At night, the temperature in the greenhouse was controlled at 12 °C, so that the temperature difference between the daytime and nighttime in the greenhouse reached 8 °C, and the temperature difference lasted for 8 days. In the fruiting management stage of tea tree mushroom, by artificially regulating the temperature difference between day and night, dry and wet difference and light source irradiation in the greenhouse, the budding cycle of tea tree mushroom can be greatly shortened, and the rapid growth of bacteria can be promoted to increase the yield of tea tree mushroom.

[0044] Further, the scattering light source is an LED light source.

[0045] Further, between step a and step b of fruiting management, a nutrient solution is injected into the mycelium in the fungus bag, and the raw materials of the nutrient solution and their mass fractions are: 4-7 parts of sucrose, 3-7 parts of chitosan. 4 parts, 1-2 parts of magnesium carbonate, 1-2 parts of sodium chloride and 50-60 parts of water. In this embodiment, it is preferable that the raw materials of the nutrient solution and their mass fractions are: 4 parts of sucrose, 3 parts of chitosan, 1 part of magnesium carbonate, 1 part of sodium chloride and 50 parts of water. The present invention prepares a nutrient solution from raw materials such as chitosan, magnesium carbonate and sodium chloride and injects it into the tea tree mushroom, which can be easily absorbed by the tea tree mushroom, effectively improves the nutritional content of the tea tree mushroom, thereby improves the reproduction efficiency and yield of the tea tree mushroom, and has low cost. High economic benefits.

[0046] c: When the mycelium in the fungus bag becomes a fruiting body, reduce the light intensity of the scattered light source to 400-800Lux, and control the temperature in the greenhouse at 20-26 °C, until the mycosis of the fruiting body turns white and the surface of the fungus cap becomes The tea tree mushrooms can be picked after 3 to 4 days after ventilation three times a day for 20 to 40 minutes each time. In this embodiment, it is preferable to reduce the light intensity of the scattered light source to 400Lux, and the temperature in the greenhouse is controlled at 20°C until the mycosis of the fruiting body turns white and the surface of the fungus cap turns red. Tea tree mushrooms can be picked after 3 days. The tea tree mushroom cultivated by the method is complete in fruiting body, rich in nutrition, sweet and refreshing in taste, and has great edible value.

[0047] Example 2

[0048] The present embodiment provides a method for cultivating Camellia sinensis using the Camellia fruit stem raw material as a culture medium: comprising the following steps:

[0049] 1) Preparation of culture medium: the raw materials of the culture medium and their mass fractions are: 35-45% of camellia stalk, 25-35% of sawdust, 8-12% of wheat husk, 8-12% of corn flour and 1-3% of quartz. %, after the raw materials of the culture medium are evenly stirred, then according to the ratio of the material to water ratio of 1:1.1 to 1.13, add water and stir to obtain the culture medium; wherein, the preparation method of the camellia fruit stem is: After being pulverized by a crusher, the particles of the camellia stem are washed and then dried, and then the particles of the camellia stem are sieved and filtered to obtain the camellia stem particles with a particle size of 0.2-0.8 cm. In the present embodiment, the particle size of the camellia fruit stem is 0.8 cm, and the camellia fruit stem raw material selected for the medium is rich in nutrients and has high content of protein and fat, so that the stem of the camellia mushroom develops more sturdy, thereby improving the output of the camellia mushroom. .

[0050] Further, the raw materials of the culture medium and their mass fractions are: 42% of camellia fruit stems, 32% of sawdust, 14% of wheat husks, 10% of corn flour and 2% of quartz. Then add water in a ratio of 1:1.1 to obtain a culture medium.

[0051] Further, the wood chips are wood chips that have been sun-aged for 30 to 40 days, and the length of the wood chips is 0.1 to 0.7 cm. In this embodiment, more preferably, the wood chips are wood chips that have been sun-aged for 40 days, and the length of the wood chips is 0.7 cm. The wood chips after sun aging treatment are easy to decompose polysaccharides and monosaccharides. Sugars can provide carbon source for tea tree mushroom strains and are easily absorbed by tea tree mushrooms, thereby improving the edible taste of tea tree mushrooms and making them more sweet and refreshing. The particles of the raw materials used in the medium are smaller, so that the mixing of the raw materials is more sufficient, which is convenient for the root system of the tea tree mushroom to absorb, thereby shortening the growth cycle of the tea tree mushroom and increasing the yield.

[0052] Further, the water in the culture medium is condensed distilled water boiled at high temperature.

[0053] Further, the pH value of the culture medium is 5-6. In this example, the pH value of the culture medium was 6. A slightly acidic medium can be more conducive to the growth and reproduction of the tea tree mushroom, thereby increasing the yield of the tea tree mushroom.

[0054] 2) Sterilization treatment: After the culture medium is packaged with a bacterial bag, the bacterial bag is put into a sterilization room for sterilization, the sterilization temperature is 100-120°C, and the sterilization time is 9-12 hours. In this embodiment, it is more preferable that the sterilization temperature is 100° C., and the sterilization time is 12 hours.

[0055] Further, the bacteria bag is a polyethylene plastic bag.

[0056] 3) Tea tree mushroom inoculation: after the sterilization is completed, the bacteria bag is naturally cooled to normal temperature, placed in an inoculation box, an inoculation hole is opened on the bacteria bag, and the tea tree mushroom strain is put into the inoculation hole for aseptic inoculation.

[0057] 4) Mycelium culture: After the inoculation is completed, place the fungus bag in the growth chamber for fungus culture until the mycelium is filled with the fungus bag. During the process of mycelium cultivation, the temperature of the fungus chamber is controlled within 21-27°C. , keep the room ventilated and dry. In this embodiment, it is preferable that the temperature of the germ-generating chamber is controlled at 27°C.

[0058] 5) Fruiting management: after the mushroom bag is covered with mycelium, the bag mouth of the mushroom bag is opened and placed in a greenhouse for fruiting management. The fruiting management includes the following steps:

[0059] a: Open the fungus bag and remove the old fungus and hyphae on the surface of the hyphae.

[0060] b: Straighten the bag mouth of the bacteria bag, and cover the bag mouth with newspaper, and spray the newspaper three times a day to raise the relative humidity of the air in the bacteria bag to 80-90%. Scattering light source, the light intensity of the scattering light source is 1200-1800Lux, the scattering light source is located above the bacteria bag, and the distance between the scattering light source and the bacteria bag is 80-130cm, and the scattering light source is turned off at night to keep the shed in a dark state. The temperature in the greenhouse is controlled between 20 and 26 °C, and at night, the temperature in the greenhouse is controlled between 12 and 18 °C, so that the temperature difference between daytime and night in the greenhouse reaches 6 to 8 °C, and the temperature difference lasts for 8 to 12 days. In this embodiment, it is preferable that the relative humidity of the air in the bacterial bag is increased to 90%, the light intensity of the scattered light source is 1800Lux, the distance between the scattered light source and the bacterial bag is 130cm, and the temperature in the greenhouse is controlled at 26°C during the day. At night, the temperature in the greenhouse was controlled at 18 °C, so that the temperature difference between the daytime and nighttime in the greenhouse reached 8 °C, and the temperature difference lasted for 12 days. In the fruiting management stage of tea tree mushroom, by artificially regulating the temperature difference between day and night, dry and wet difference and light source irradiation in the greenhouse, the budding cycle of tea tree mushroom can be greatly shortened, and the rapid growth of bacteria can be promoted to increase the yield of tea tree mushroom.

[0061] Further, the scattering light source is an LED light source.

[0062] Further, between step a and step b of fruiting management, a nutrient solution is injected into the mycelium in the fungus bag, and the raw materials of the nutrient solution and their mass fractions are: 4-7 parts of sucrose, 3-7 parts of chitosan. 4 parts, 1-2 parts of magnesium carbonate, 1-2 parts of sodium chloride and 50-60 parts of water. In this embodiment, the more preferred raw materials and mass fractions of the nutrient solution are: 7 parts of sucrose, 4 parts of chitosan, 2 parts of magnesium carbonate, 2 parts of sodium chloride and 60 parts of water. The present invention prepares a nutrient solution from raw materials such as chitosan, magnesium carbonate and sodium chloride and injects it into the tea tree mushroom, which can be easily absorbed by the tea tree mushroom, effectively improves the nutritional content of the tea tree mushroom, thereby improves the reproduction efficiency and yield of the tea tree mushroom, and has low cost. High economic benefits.

[0063] c: When the mycelium in the fungus bag becomes a fruiting body, reduce the light intensity of the scattered light source to 400-800Lux, and control the temperature in the greenhouse at 20-26 °C, until the mycosis of the fruiting body turns white and the surface of the fungus cap becomes The tea tree mushrooms can be picked after 3 to 4 days after ventilation three times a day for 20 to 40 minutes each time. In this embodiment, it is preferable to reduce the light intensity of the scattered light source to 800Lux, and the temperature in the greenhouse is controlled at 26°C until the fungal disease of the fruiting body turns white and the surface of the fungus cap turns red. Tea tree mushrooms can be picked after 4 days. The tea tree mushroom cultivated by the method is complete in fruiting body, rich in nutrition, sweet and refreshing in taste, and has great edible value.

[0064] Compared with the prior art, the present invention has the following beneficial effects:

[0065] 1, the present invention selects camellia fruit stalk and aging sawdust as the raw material of the culture medium, the camellia fruit stalk is nutritious and has high-content protein and fat, so that the stem of the tea tree mushroom is developed more sturdy, thereby improving the output of the tea tree mushroom; Sun-aging treated wood chips are easy to decompose polysaccharides and monosaccharides. Sugars can provide carbon source for tea tree mushroom strains and are easily absorbed by tea tree mushrooms, thereby improving the edible taste of tea tree mushrooms and making them more sweet and refreshing; the medium is selected from raw materials The particles are smaller, which makes the mixing between the raw materials more sufficient, which is convenient for the root system to absorb the tea tree mushroom, thereby shortening the growth cycle of the tea tree mushroom and increasing the yield;

[0066] 2. In the fruiting management stage of the tea tree mushroom, the present invention can greatly shorten the bud-promoting period of the tea tree mushroom by artificially regulating the temperature difference between day and night, the dry-humidity difference and the light source irradiation in the greenhouse, and promote the rapid growth of the bacteria, so as to improve the quality of the tea tree mushroom. output;

[0067] 3. The present invention uses raw materials such as chitosan, magnesium carbonate and sodium chloride to prepare a nutrient solution and inject it into the tea tree mushroom, which can be easily absorbed by the tea tree mushroom, effectively improve the nutritional content of the tea tree mushroom, thereby improving the reproductive efficiency and output of the tea tree mushroom, and the cost low cost and high economic efficiency;

[0068] 4. The tea tree mushroom cultivated by the present invention has complete fruiting body, rich nutrition, sweet and refreshing taste, and has great edible value.