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A preparation method of chromatographic packing based on capillary droplet microfluidics

A chromatographic packing and capillary technology, which is applied in the field of chromatographic packing preparation based on capillary droplet microfluidics, can solve the problems of reducing the chromatographic separation performance of packing, and difficulty in preparing monodisperse chromatographic packing.

Active Publication Date: 2022-04-26
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional packing preparation techniques (including sol-gel method, spray drying method and microemulsion method, etc.) are difficult to prepare monodisperse and uniform chromatographic packing, and the size distribution of packing is in the range of CV=10-20%, thereby reducing the Chromatographic separation performance; therefore, the preparation of chromatographic packing materials with excellent monodispersity has become an urgent need

Method used

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  • A preparation method of chromatographic packing based on capillary droplet microfluidics
  • A preparation method of chromatographic packing based on capillary droplet microfluidics
  • A preparation method of chromatographic packing based on capillary droplet microfluidics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation method of boric acid microspheres based on capillary droplet microfluidic technology

[0035] 1. Configuration of dispersed phase and continuous phase

[0036]Dispersed phase: first prepare the silane hydrolyzate, mix 360 μL TMOS, 400 μL GPTMS, 1 mL 0.01M acetic acid and 50 μL PEG 200, stir in an ice-water bath for 1 hour, then mix 10 mg APBA and 250 μL hydrolyzate to complete the preparation of the dispersed phase.

[0037] Continuous phase: n-hexadecane and 10 wt% of EM90 were mixed as the continuous phase.

[0038] 2. Construction of capillary droplet microfluidic platform

[0039] The construction of the capillary droplet microfluidic platform is mainly divided into the following steps: (1) The drawing of the capillary tip: the capillary tip is drawn on the butane flame, the capillary is first drawn thin at the outer flame of the flame, and then the inner The capillary is pulled off at the flame to form a capillary tip, such as figure 2 sh...

Embodiment 2

[0044] Example 2: Preparation method of hybrid silica gel microspheres based on capillary droplet microfluidic technology

[0045] 1. Configuration of dispersed phase and continuous phase

[0046] Dispersed phase: 223μL TMOS, 378μL BTME, 1.5mL 0.01M acetic acid, 100μL PEG 200 and 0.16g urea, stirred in an ice-water bath for 1h;

[0047] Continuous phase: n-hexadecane and 10 wt% of EM90 were mixed as the continuous phase.

[0048] 2. Droplet generation

[0049] A capillary droplet microfluidic platform was constructed in the manner of Example 1, and monodisperse droplets were generated.

[0050] 3. Droplets solidify to form microspheres

[0051] Collect the generated droplets into a centrifuge tube, react at 40°C for 14h to solidify, and then raise the temperature to 120°C for 7h to decompose urea. The resulting microspheres were then ultrasonically cleaned with hexane and methanol, respectively.

[0052] 4. Calcination of microspheres

[0053] Carry out high-temperature ...

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Abstract

The invention relates to a preparation method of a chromatographic filler based on capillary droplet microfluidics, which relates to a chromatographic filler. 1) Construction of capillary droplet microfluidic platform: combine tee, capillary, syringe and syringe pump to construct droplet microfluidic platform; 2) droplet generation: introduce the prepared reaction solution as a dispersed phase In the droplet microfluidic device, the immiscible continuous phase is used for cutting to generate monodisperse droplets; 3) microsphere solidification: the chromatographic filler can be obtained after the liquid droplets are used as templates and solidified. The construction of the capillary droplet microfluidic platform does not require photolithography and ultra-clean room, and can be constructed through the combination of capillary and tee, which greatly saves costs and can stably generate smaller-sized droplets. It can greatly improve the uniformity of the filler, and adjust the chemical composition of the stationary phase of the filler by adjusting the chemical composition of the reaction solution, and adjust the size and shape of the generated filler by adjusting the size and shape of the droplet, so as to achieve the precision of the chromatographic filler. manufacture.

Description

technical field [0001] The invention relates to a chromatographic filler, in particular to a method for preparing a chromatographic filler based on capillary droplet microfluidics. Background technique [0002] With the development of life science, people's research on the life system is deepening day by day. Especially with the arrival of the post-genomics era, people's attention began to turn to proteomics and metabolomics. Proteomics and metabolomics samples are extremely complex, which puts forward extremely high requirements for separation and analysis methods. Due to its excellent resolving power, HPLC has become an ideal choice for high-resolution separation and analysis of complex systems. Chromatographic packing is the core part of high performance liquid chromatography, and its properties will directly affect the selectivity and resolution of chromatographic separation. [0003] The separation performance of a chromatography packing is directly related to the si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/281B01J20/30B01L3/00B01J13/02
CPCB01J20/281B01J20/3028B01L3/5027B01J13/02B01J2220/52B01J2220/54
Inventor 张博刘娅
Owner XIAMEN UNIV