Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gelatin-silk fibroin hydrogel loaded with adipose-derived stem cells and plasma as well as preparation method and application of gelatin-silk fibroin hydrogel

An adipose stem cell and silk fibroin technology, applied in the field of gelatin-silk fibroin hydrogel and its preparation, can solve problems such as short retention time, and achieve excellent biocompatibility, good mechanical properties, and slow biodegradability. Effect

Pending Publication Date: 2021-06-29
BEOGENE BIOTECH GUANGZHOU
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although ADSCs and PRP are therapeutically effective, they remain in vivo for a short time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gelatin-silk fibroin hydrogel loaded with adipose-derived stem cells and plasma as well as preparation method and application of gelatin-silk fibroin hydrogel
  • Gelatin-silk fibroin hydrogel loaded with adipose-derived stem cells and plasma as well as preparation method and application of gelatin-silk fibroin hydrogel
  • Gelatin-silk fibroin hydrogel loaded with adipose-derived stem cells and plasma as well as preparation method and application of gelatin-silk fibroin hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of embodiment 1, methacrylated gelatin (GelMA)

[0048] Dissolve 10g of gelatin in 100mL of PBS solution at a temperature of 60°C until completely dissolved to obtain a mixture, then add 12mL of methacrylic anhydride (MA) to the mixture at a temperature of 50°C for 4 to 12 hours until it becomes It is milky white, and finally uses a dialysis bag with a molecular weight cut-off of 8000-15000Da to dialyze with deionized water for 5 days to remove unreacted methacrylic anhydride and other by-products. The resulting solution is freeze-dried to obtain methacrylated gelatin, and placed in Store at -20°C for further use.

Embodiment 2

[0049] Embodiment 2, the preparation of methacrylated silk fibroin (SFMA)

[0050] The preparation method of methacrylated silk fibroin (SFMA), comprises the following steps:

[0051] 1) Place 50g silkworm cocoons in 500mL 0.1M Na 2 CO 3 Boil in the solution for 20 minutes at a boiling temperature of 100°C to remove the sericin, then ultrasonically wash it in deionized water for 3 times to obtain the degummed silk fibroin, and then place it in an oven at a temperature of 60°C to over-dry to a constant weight;

[0052] 2) Mix 20 g of dried degummed silk fibroin into 100 mL of lithium bromide solution (9.3 mol / L) until the degummed silk fibroin is completely dissolved to obtain a silk fibroin high-salt solution, and magnetically stir the silk fibroin high-salt solution at room temperature. Saline solution, stirred for 8 hours, and 6 mL of glycidyl methacrylate was added dropwise to the silk fibroin high-salt solution to obtain a mixed solution;

[0053] 3) Filter the mixed ...

Embodiment 3

[0054] The preparation of embodiment 3, GelMA / 10%SFMA hydrogel

[0055] Get the GelMA (10wt.%) prepared by 0.2g embodiment 1, the SFMA (10wt.%) prepared by 0.2g embodiment 2 and the photoinitiator (0.1wt.%) of 0.002g LAP are dissolved in 2mL deionized water, blue light After 10 seconds, the GelMA / 10% SFMA hydrogel was obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of medical biological materials, and particularly discloses gelatin-silk fibroin hydrogel loaded with adipose-derived stem cells and plasma as well as a preparation method and application of the gelatin-silk fibroin hydrogel. The hydrogel is prepared from the following materials: methacrylic acid gelatin, methacrylic acid silk fibroin and a photoinitiator, and in the hydrogel, the mass ratio of the methacrylic acid gelatin to the methacrylic acid silk fibroin to the photoinitiator is (100-150):(100-150):(0.5-1). The hydrogel prepared by the invention not only has stable rheological property, slow biodegradability and better mechanical property, but also can continuously release growth factors (the continuous release rate reaches 14 days) and obviously promote the proliferation performance of the adipose-derived stem cells, and the adipose-derived stem cells wrapped in the hydrogel have higher survival time, so that the growth factors can be continuously released, nd continuous proliferation can be realized in the hydrogel.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a gelatin-silk fibroin hydrogel loaded with fat stem cells and blood plasma, a preparation method and application thereof. Background technique [0002] Emaciated elderly patients often develop pressure ulcers due to prolonged paralysis and bed rest. The appearance of pressure ulcers is usually due to the long-term pressure without adipose tissue protection. Pressure sores on the buttocks are more common among patients. Pressure ulcers not only bring a heavy financial burden to patients, but also aggravate their physical pain and even cause life-threatening complications. As the population ages, the incidence of pressure ulcers increases year by year. Effective treatment of pressure ulcers is an unsolved problem. At present, the more traditional treatment method is postoperative fixation and bandaging with gauze and cotton pads. However, the inconvenience of post...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08F299/00C08F2/48A61L26/00
CPCC08F299/00C08F2/48A61L26/0038A61L26/0047A61L26/0057A61L26/0066A61L26/008A61L26/0085A61L26/009A61L2300/30A61L2300/412A61L2300/602
Inventor 郭瑞蓝咏刘玉胡圣学李可可卢魁
Owner BEOGENE BIOTECH GUANGZHOU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com