Application of NtFER gene-based modification in improvement of bacterial wilt resistance of plants

A bacterial wilt and gene technology, applied in the application field of improving plant resistance, can solve the problems of complex infection sources and many types of variation, and achieve obvious resistance and improve the effect of resistance

Pending Publication Date: 2021-06-29
HUNAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still many problems in the chemical and biological control of tobacco bacterial wilt, and there are many variant types of R. solanacearum strains, and the source of infection is relatively complicated. effective treatment for the disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of NtFER gene-based modification in improvement of bacterial wilt resistance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention , but not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0024] The tobacco ecotype is Yunyan 87; the Agrobacterium strain is LBA4404; the vector is pORE-Cas9 / gRNA; the main reagents include: restriction endonuclease from Thermo Fisher, DNA polymerase from Novizyme, Infusion ligase, etc.; Reverse transcription kit from Thermo Company; RNA extraction kit from Tiangen Company; plasmid extraction kit and DNA recovery kit from Tiangen Company;...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to application of NtFER-based transformation in improvement of plant resistance. According to the invention, the tobacco NtFER gene is taken as a target, transformation is carried out through a genetic engineering means, the expression quantity of the NtFER gene is reduced, and the resistance of plants to bacterial wilt is improved. Specifically, the tobacco NtFER gene is used as a target, an sgRNA sequence of CRISPR / Cas9 is designed, a DNA fragment containing the sgRNA is connected into a CRISPR / Cas9 carrier, then tobacco is transformed, an editing material of the tobacco NtFER gene is obtained, and it is found that the editing material has obvious resistance to bacterial wilt.

Description

technical field [0001] The invention belongs to the technical field of plant disease prevention and control, and in particular relates to the application of NtFER gene-based transformation in improving plant resistance. Background technique [0002] FERONIA (FER) is a kind of receptor protein kinase conserved in the plant kingdom. It is found in plants that it has the function of responding to biotic and abiotic stresses, including cold and heat stress, salt stress, pathogenic bacteria stress, etc. Especially in recent years, it has been found that its ligand molecule RALF23 can inhibit the plant immune system through FERONIA, and in the roots of plants, root-knot nematodes can inhibit the plant immune system by secreting RALF analogues and FERONIA, so FER plays an important role in the regulation of plant immunity. play an important role in. In tobacco, it has been found that NtFER is mainly involved in the regulation of tobacco leaf size and aroma substances. [0003] Ra...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/82
CPCC07K14/415C12N15/8281C12N15/8216
Inventor 汪龙于峰伍斗生李晓旭刘红斌
Owner HUNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap