Application of gene TSA in detection of collybia albuminosa derived components
A technology of derived components and genes, applied in the field of biological species identification and PCR detection, to achieve the effect of less time-consuming, good specificity and simple operation
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[0016] Example 1: Chicken Source Universal Internal Standard Gene TSA Screening
[0017] In the NCBI's Nucleotide database, the gene information of the chicken is searching, and the glycosidase is finally screened by the BLAST alignment analysis. TSA Gene (login number: jo124034.1) as an internal standard gene, TSA Gene's BLAST results and details figure 1 , TSA The BLAST alignment of the gene is shown in the drawing of all sequences of any other genes. TSA The sequence of the gene is the same or similar, this shows TSA Genes have no homology with other genes, which has strong species specificity.
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[0018] Example 2: Verification of geosetic components generalized internal standard gene
[0019] 1. Internal gene screening for Example 1 TSA The primer is designed with Primer Premier 5.0 software, and BLAST comparison analysis is performed on the design of the primer to ensure the specificity of the primer, the primer sequence is shown in Table 1; the amplified sequence is the 213th base of SEQ ID NO: 1 ~ 546 bases;
[0020] Table 1 TSA Gene's PCR primer sequence
[0021] ;
[0022] 2, qualitative verification of standard gene primer species in chicken
[0023] Take the yellow boys, northern wind bacteria, black bovine liver, milk pump, green bacteria, drum, chicken oil, pine, red, white bolet, big red mushroom, yellow raft, chicken, old man , Coral bacteria, mushrooms, tea trees, apricot emerrenus, genomic DNA of the mushrooms as a template, use primers TSA -F / b conducts normal PCR amplification detection, qualitatively verifies the species specificity of standard genes in...
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