Application of gene TSA in detection of collybia albuminosa derived components

A technology of derived components and genes, applied in the field of biological species identification and PCR detection, to achieve the effect of less time-consuming, good specificity and simple operation

Pending Publication Date: 2021-06-29
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the internal standard gene of chicken fir has not been seen relevant report, in order to establish a kind of method t

Method used

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  • Application of gene TSA in detection of collybia albuminosa derived components
  • Application of gene TSA in detection of collybia albuminosa derived components
  • Application of gene TSA in detection of collybia albuminosa derived components

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1: Chicken Source Universal Internal Standard Gene TSA Screening

[0017] In the NCBI's Nucleotide database, the gene information of the chicken is searching, and the glycosidase is finally screened by the BLAST alignment analysis. TSA Gene (login number: jo124034.1) as an internal standard gene, TSA Gene's BLAST results and details figure 1 , TSA The BLAST alignment of the gene is shown in the drawing of all sequences of any other genes. TSA The sequence of the gene is the same or similar, this shows TSA Genes have no homology with other genes, which has strong species specificity.

Example Embodiment

[0018] Example 2: Verification of geosetic components generalized internal standard gene

[0019] 1. Internal gene screening for Example 1 TSA The primer is designed with Primer Premier 5.0 software, and BLAST comparison analysis is performed on the design of the primer to ensure the specificity of the primer, the primer sequence is shown in Table 1; the amplified sequence is the 213th base of SEQ ID NO: 1 ~ 546 bases;

[0020] Table 1 TSA Gene's PCR primer sequence

[0021] ;

[0022] 2, qualitative verification of standard gene primer species in chicken

[0023] Take the yellow boys, northern wind bacteria, black bovine liver, milk pump, green bacteria, drum, chicken oil, pine, red, white bolet, big red mushroom, yellow raft, chicken, old man , Coral bacteria, mushrooms, tea trees, apricot emerrenus, genomic DNA of the mushrooms as a template, use primers TSA -F / b conducts normal PCR amplification detection, qualitatively verifies the species specificity of standard genes in...

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Abstract

The invention discloses an application of gene TSA in detection of collybia albuminosa derived component. A set of qualitative and quantitative PCR detection method for collybia albuminosa is established, a deep processing product of collybia albuminosaisdetected and verified, analysis finds that a BLAST comparison result graph of the TSA gene shows that all sequences of other genes are not the same as or similar to the sequence of the TSA gene, which indicates that the TSA gene does not have homology with other genes and has extremely high species specificity, so that the TSA gene is selected as a candidate endogenous reference gene of thecollybia albuminosa, and the collybia albuminosa-derived components in food can be rapidly and sensitively detected. The method is high in sensitivity, high in specificity, simple to operate, low in cost, easy in reaction result observation and very suitable for on-site real-time detection, and has good development prospects in rapid biological source detection of collybia albuminosa and deeply processed products thereof.

Description

technical field [0001] The invention relates to the technical field of biological species identification and PCR detection, in particular to a gene TSA Application in the detection of components derived from chicken fir. Background technique [0002] Chicken fir ( Collybia albuminosa ) Because it tastes like chicken, it is also known as chicken silk fungus. It is mainly distributed in the tropics of Asia and Africa, the islands of the South Pacific and the subtropical regions of Asia. In my country, it is mainly produced in Yunnan, Guizhou, Sichuan, Taiwan, Tibet and other places. Chicken fir is rich in amino acids, riboflavin, K, Fe, Cu, Mg, Zn, Ca and other mineral elements. The protein and total sugar content reached 32.82% and 36.79% respectively. It is a delicious and nutritious food. Highly valuable edible fungi. However, due to its complex symbiotic relationship with termites, the growth environment of fir is extremely harsh, and there is no report of artificial ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/158C12Q2600/166
Inventor 商颖曹建新易伦朝刘耀李玲魏元苗
Owner KUNMING UNIV OF SCI & TECH
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