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Application of gene TSA in detection of collybia albuminosa derived components

A technology of derived components and genes, applied in the field of biological species identification and PCR detection, to achieve the effect of less time-consuming, good specificity and simple operation

Pending Publication Date: 2021-06-29
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the internal standard gene of chicken fir has not been seen relevant report, in order to establish a kind of method that can quickly and accurately identify chicken fir, it is necessary to develop the internal standard gene of chicken fir

Method used

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  • Application of gene TSA in detection of collybia albuminosa derived components
  • Application of gene TSA in detection of collybia albuminosa derived components
  • Application of gene TSA in detection of collybia albuminosa derived components

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Embodiment 1: Common internal standard gene of chicken fir source TSA screening

[0017] In the Nucleotide database of NCBI, search for the gene information of cyprinus fir, and finally screen out the genes used to encode glycosidase through BLAST comparison analysis. TSA Gene (accession number: JO124034.1) was used as an internal standard gene, TSA BLAST results and details for genes are in figure 1 , TSA The BLAST alignment results of the gene show that there is no complete sequence of any other gene with TSA The sequences of the genes are identical or similar, which means that TSA The gene has no homology with other genes and has strong species specificity.

Embodiment 2

[0018] Embodiment 2: Verification of the universal internal standard gene of the origin component of chicken fir

[0019] 1. For the internal gene screened in Example 1 TSA , use the Primer Premier 5.0 software to design primers, and perform BLAST comparative analysis on the designed primers to ensure the specificity of the primers. The primer sequences are shown in Table 1; the amplified sequence is the 213th base to the 1st base of SEQ ID NO:1 546 bases;

[0020] Table 1 TSA Gene PCR primer sequence

[0021] ;

[0022] 2. Qualitative verification of the species-specificity of the standard gene primers in C. fir

[0023] Yellow boletus liver, northern wind fungus, black bovine liver, milk milk fungus, blue head fungus, dry mushroom, chanterelle mushroom, matsutake, red chisel, white boletus, red mushroom, yellow raisin, chicken fir, old man's head , coral fungi, shiitake mushrooms, tea tree mushrooms, pleurotus eryngii, Tricholoma eryngii as templates, using primers T...

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Abstract

The invention discloses an application of gene TSA in detection of collybia albuminosa derived component. A set of qualitative and quantitative PCR detection method for collybia albuminosa is established, a deep processing product of collybia albuminosaisdetected and verified, analysis finds that a BLAST comparison result graph of the TSA gene shows that all sequences of other genes are not the same as or similar to the sequence of the TSA gene, which indicates that the TSA gene does not have homology with other genes and has extremely high species specificity, so that the TSA gene is selected as a candidate endogenous reference gene of thecollybia albuminosa, and the collybia albuminosa-derived components in food can be rapidly and sensitively detected. The method is high in sensitivity, high in specificity, simple to operate, low in cost, easy in reaction result observation and very suitable for on-site real-time detection, and has good development prospects in rapid biological source detection of collybia albuminosa and deeply processed products thereof.

Description

technical field [0001] The invention relates to the technical field of biological species identification and PCR detection, in particular to a gene TSA Application in the detection of components derived from chicken fir. Background technique [0002] Chicken fir ( Collybia albuminosa ) Because it tastes like chicken, it is also known as chicken silk fungus. It is mainly distributed in the tropics of Asia and Africa, the islands of the South Pacific and the subtropical regions of Asia. In my country, it is mainly produced in Yunnan, Guizhou, Sichuan, Taiwan, Tibet and other places. Chicken fir is rich in amino acids, riboflavin, K, Fe, Cu, Mg, Zn, Ca and other mineral elements. The protein and total sugar content reached 32.82% and 36.79% respectively. It is a delicious and nutritious food. Highly valuable edible fungi. However, due to its complex symbiotic relationship with termites, the growth environment of fir is extremely harsh, and there is no report of artificial ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/158C12Q2600/166
Inventor 商颖曹建新易伦朝刘耀李玲魏元苗
Owner KUNMING UNIV OF SCI & TECH
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