Application of gene TSA in detection of collybia albuminosa derived components
A technology of derived components and genes, applied in the field of biological species identification and PCR detection, to achieve the effect of less time-consuming, good specificity and simple operation
Pending Publication Date: 2021-06-29
KUNMING UNIV OF SCI & TECH
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The invention discloses an application of gene TSA in detection of collybia albuminosa derived component. A set of qualitative and quantitative PCR detection method for collybia albuminosa is established, a deep processing product of collybia albuminosaisdetected and verified, analysis finds that a BLAST comparison result graph of the TSA gene shows that all sequences of other genes are not the same as or similar to the sequence of the TSA gene, which indicates that the TSA gene does not have homology with other genes and has extremely high species specificity, so that the TSA gene is selected as a candidate endogenous reference gene of thecollybia albuminosa, and the collybia albuminosa-derived components in food can be rapidly and sensitively detected. The method is high in sensitivity, high in specificity, simple to operate, low in cost, easy in reaction result observation and very suitable for on-site real-time detection, and has good development prospects in rapid biological source detection of collybia albuminosa and deeply processed products thereof.
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