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A nucleic acid detection method for food-borne pathogens based on hollow gold-silver core-shell nanoflower SERS nanoprobe HAU/AGSNFS-ATP

A gold-silver core-shell and nano-probe technology, which is applied in the field of photochemical analysis technology and biological detection, to meet the needs of on-site detection, high sensitivity and specificity detection, and achieve the effect of portability

Active Publication Date: 2022-04-29
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nucleic acid detection is widely used in various aspects, such as early tumor nucleic acid marker detection, rapid identification in criminal investigation, rapid detection of food safety, rapid monitoring of bacteria and viruses in the environment, vaccine safety detection, screening of blood infectious diseases etc. Therefore, it is of great significance to establish a general nucleic acid rapid detection method, but a general and highly sensitive nucleic acid rapid detection method has not been reported yet.

Method used

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  • A nucleic acid detection method for food-borne pathogens based on hollow gold-silver core-shell nanoflower SERS nanoprobe HAU/AGSNFS-ATP
  • A nucleic acid detection method for food-borne pathogens based on hollow gold-silver core-shell nanoflower SERS nanoprobe HAU/AGSNFS-ATP
  • A nucleic acid detection method for food-borne pathogens based on hollow gold-silver core-shell nanoflower SERS nanoprobe HAU/AGSNFS-ATP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of SERS nanoprobe HAu / AgSNFs-ATP and establishment of paper-based SERS sensing rapid detection method

[0038] Step 1, HAu / AgSNFs preparation: in 1mL H 3 NO solution (1×10 -2 -6×10 -2M) and 1mL NaOH solution (0.01-0.08M) mixed with 18mL AgNO 3 (0.1-1 M) solution was stirred for 5-20min, then 10mL of HAuCl was added 4 solution (2×10 -4 -10×10 -4 M) and 200 μL C 6 h 5 Na 3 o 7 The solution (0.5%-1.5%, w / v) was reacted at 80-110°C for 5 minutes to obtain HAu / AgSNFs.

[0039] Step 2, functional modification of HAu / AgSNFs: add 30 μL C to 10 mL HAu / AgSNFs aqueous solution 6 h 7 NS alcohol-water mixed solution (0.01-1M), centrifuged after stirring for 15 minutes, washed twice, and finally dispersed in aqueous solution to obtain SERS nanoprobe based on HAu / AgSNFs-ATP.

[0040] Different AgNO 3 The transmission electron microscope images of the HAu / AgSNFs nanoprobes prepared under the concentration figure 1 shown by figure 1 As can be seen, A...

Embodiment 2

[0046] Example 2 Application of Paper-based SERS Sensing Rapid Detection Method

[0047] 2.1 Application of paper-based SERS sensing rapid detection method in bacterial detection

[0048] 2.1.1 Take Staphylococcus aureus, Escherichia coli and Salmonella as examples to conduct qualitative and quantitative detection of bacteria

[0049] 2.1.1.1 Staphylococcus aureus

[0050] Heat the Staphylococcus aureus with a concentration of 0-7cfu / mL to extract the crude DNA, add the crude extract dropwise to the pretreatment area to adsorb the interfering substances in the reaction system, and then fold the device to combine the pretreatment area and the amplification The region overlaps, and the gene amplification is carried out; the total volume of the amplification reaction is 20 μL, which contains a pair of primers P1 and P2 with concentrations of 10 -6 M, the system also includes a concentration of 5 × 10 -4 M dNTPs, 1×Isothermopol Buffer, and Bst 2.0 WarmStart DNA polymerase; the ...

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Abstract

The invention discloses a hollow gold-silver core-shell nanoflower SERS nanoprobe HAu / AgSNFs-ATP, which relates to the field of photochemical analysis technology and biological detection; the invention also discloses a nucleic acid qualitative and / or based on magenta sulfurous acid reagent and SERS Or quantitative detection sensor, through nano-controllable self-assembly to prepare hollow gold-silver core-shell nano-snowflake SERS nanoprobes, combined with chemometric methods and colorimetry to build a steady-state nucleic acid visualization and accurate quantitative model, and established a universal nucleic acid detection The method realizes the portability, high sensitivity and specificity detection of various nucleic acids.

Description

technical field [0001] The invention relates to the fields of photochemical analysis technology and biological detection, in particular to a hollow gold-silver core-shell nanoflower SERS nanoprobe HAu / AgSNFs-ATP. Background technique [0002] Surface-Enhanced Raman Scattering (SERS) refers to the enhancement of the electromagnetic field on the surface or near the surface of the sample, resulting in the adsorption of molecules on the surface or sol of some specially prepared metal good conductors. Raman scattering signal is greatly enhanced than normal Raman scattering (NRS) signal. SERS overcomes the shortcomings of low sensitivity of Raman spectroscopy, and can obtain structural information that is not easily obtained by conventional Raman spectroscopy. Structural analysis, etc., can effectively analyze the adsorption orientation of the compound on the interface, the change of the adsorption state, and the interface information. At present, SERS has been widely used in ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65C12Q1/6825
CPCG01N21/658C12Q1/6825G01N2021/656
Inventor 陈全胜许艺李欢欢焦天慧朱阿芳
Owner JIANGSU UNIV