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Application of aphanothece sacrum polysaccharide in preparation of medicine for treating scalds

A technology of cyanobacterial polysaccharide and Shuiqian Temple, which is applied in the field of biomedicine, can solve the problem that there is still less research on the mechanism of scald repair, and achieve the effects of reducing the production amount, slowing down the acute inflammatory response, and increasing the content.

Inactive Publication Date: 2021-07-13
圣珂兰投资有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on burn repair mainly focuses on anti-inflammation, and there are still few studies on the specific mechanism of burn repair.

Method used

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  • Application of aphanothece sacrum polysaccharide in preparation of medicine for treating scalds
  • Application of aphanothece sacrum polysaccharide in preparation of medicine for treating scalds
  • Application of aphanothece sacrum polysaccharide in preparation of medicine for treating scalds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Effect of Shuiqianji Spirulina Polysaccharide on Rat Scald Healing Rate

[0050] figure 2 It is the wound recovery of different treatment groups on the 15th, 17th, 19th, and 21st day. The front wound is the control wound, and the rear wound is the treatment wound. On the 15th day, under the effect of the rat's own recovery ability, the wound has basically healed. Scab, there is a certain amount of bleeding from the wound, which may be due to unnatural shedding of the scab due to scratching, scratching and other reasons. Compared with the normal saline negative control wound, the recovery effect of the other three groups was significantly better, and the recovery effect was negative control normal saline, combined drug group, silver sulfadiazine, and Shuiqiansi cyanobacteria polysaccharide. Therefore, it can be preliminarily determined that Shuiqianji cyanobacteria polysaccharide has a therapeutic effect on the recovery of scald wounds, and the therapeutic ef...

Embodiment 2

[0052] Example 2 Effect of Shuiqianji Spirulina Polysaccharide on Initial Scald Inflammation

[0053] 1. Determination of serum inflammatory factor levels

[0054] Use enzyme-linked immunosorbent assays (Enzyme linked immunosorbent assays, ELISA) to measure the levels of TNF-α and IL-1β (inflammatory factors) in serum. The kits used below are Abcam kits (ab100767 and ab46070). Operate according to the instructions, and each group 3 test samples, 2 parallel replicates for each sample.

[0055] Sample and reagent preparation: Dilute the standard, HRP, washing buffer, IL-1β antibody, TNF-α antibody and biotin-labeled secondary antibody to 1x, and use Diluent A to pretreat the sample.

[0056] IL-1β: add 100 μL standard and sample, incubate at room temperature for 2.5 hours; wash the plate with 300 μL washing buffer for each well, wash 4 times, remove as much liquid as possible each time; add 100 μL detection antibody to each well, incubate for 1 hour, gently Shake; wash the pla...

Embodiment 3

[0064] Example 3 Effect of Shuiqiansi Spirulina Polysaccharide on Type I and Type III Collagen Tissue Distribution

[0065] A. Fixative preparation (4% paraformaldehyde)

[0066] Phosphate buffer A (0.2mol / L): Take NaH 2 PO 4 2H 2 O 27.6g dissolved in ddH 2 O, set the volume to 1L.

[0067] Phosphate buffer B (0.2mol / L): take Na 2 HPO 4 12H 2 O 71.6g dissolved in ddH 2 O, set the volume to 1L.

[0068] PB solution (0.1mol / L): take buffer A and buffer B, mix them at a ratio of 19:81, adjust the pH value to 7.4, add an equal volume of ddH 2 O and 40g of paraformaldehyde powder, heat and stir at 65°C, after completely dissolving, dilute to 1L.

[0069] B. Paraffin tissue embedding (n-butanol method)

[0070] Prepare nine 50ml centrifuge tubes, mark No. 1-9, add 50% ethanol in order; ethanol: n-butanol: water (5:2:3) mixture; ethanol: n-butanol: water (10:7:3 ) mixture; ethanol: n-butanol: water (9:9:2) mixture; ethanol: n-butanol (1:3) mixture; ethanol: n-butanol (3:1...

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Abstract

The invention provides an application of aphanothece sacrum polysaccharidein preparation of a medicine for treating scalds. When being used for treating scalds, the aphanothece sacrum polysaccharidein can lower the IL-1beta level, relieve acute inflammatory response, lower the content of Col I and increase the content of Col III in the healing process, so that scars are not easy to leave after wounds are healed.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of Shuiqianji blue-green algae polysaccharide in the preparation of medicine for treating scalds. Background technique [0002] Scald is a common type of accidental injury. The skin covers the entire body surface and is the largest organ in the body. It is generally accompanied by tissue necrosis after burns. It not only damages local tissues at the wound, but also causes systemic damage and inflammation. Known as scald disease. The measurement of the severity of burns is mainly based on the comprehensive judgment of the burn site, area size, and burn depth. It is generally divided into the following four grades: [0003] First-degree burns: the burn depth is relatively shallow, only involving skin surface damage, resulting in local mild redness and swelling of the skin wound surface, specifically manifested as dry surface of the burn site and obvious pain, n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P17/02A61P29/00
CPCA61K31/715A61P17/02A61P29/00
Inventor 左正宏辛国彬金玉兰何承勇任宋雯杨春燕周懿翕
Owner 圣珂兰投资有限公司
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