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Hydrogel scaffold with polydopamine coating and preparation method thereof

A technology of polydopamine and hydrogel, which is applied in the field of osteochondral repair, can solve problems such as loss, and achieve the effect of simple steps, low price, and high-efficiency steps

Active Publication Date: 2021-07-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in the early stage of the operation, after the subchondral bone is punctured, the bone marrow mesenchymal stem cells flow out together with the bone marrow fluid. Effective repair of osteochondral damage

Method used

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  • Hydrogel scaffold with polydopamine coating and preparation method thereof
  • Hydrogel scaffold with polydopamine coating and preparation method thereof
  • Hydrogel scaffold with polydopamine coating and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of hydrogel scaffolds with polydopamine coating.

[0044] Gelatin modification: at 50°C, 10g of gelatin was dissolved in 100ml of PBS buffer solution, and then 4ml of methacrylic anhydride was slowly added dropwise, and stirred and reacted at 50°C for 3h. Then add 4500ml of PBS to terminate the reaction, put the product in a 12000-14000DA dialysis bag, and dialyze at 40°C for 5-7 days, then freeze the product in a -80°C refrigerator, and obtain a white floc after lyophilization The product is methacrylated gelatin.

[0045] Preparation of mixed slurry for 3D printing: Add 0.05g of photoinitiator I2959 and 0.022g of anhydrous calcium chloride into 10ml of deionized water at 50°C. After completely dissolving, add 1g of modified gelatin, and then add 0.5g of alginic acid sodium. Use a stirrer to stir evenly, and then ultrasonicate at 45° C. for 30 min to obtain a mixed slurry for printing.

[0046]Printing of pre-crosslinked composite scaffolds: first transf...

Embodiment 2

[0051] Surface morphology observation of modified gelatin / sodium alginate composite scaffold with polydopamine coating.

[0052] The modified gelatin / sodium alginate composite scaffold with polydopamine coating was prepared according to Example 1. Paste the stent sample on the conductive tape, and observe it with a tungsten filament scanning electron microscope after spraying gold for 60 seconds. Such as figure 1 As shown in (a), the lines of the scaffold without polydopamine coating are relatively smooth, which is not conducive to cell adhesion, such as figure 1 As shown in (b), the surface morphology of the sample with polydopamine coating becomes uneven due to the adhesion of many polydopamine microspheres, and the uneven surface is more conducive to cell adhesion.

Embodiment 3

[0054] Mechanical properties testing of modified gelatin / sodium alginate composite scaffolds with polydopamine coating.

[0055] The modified gelatin / sodium alginate composite scaffold with polydopamine coating was prepared according to Example 1. Swell the stent sample in deionized water at 37°C. After the swelling is balanced, take out the stent sample, gently wipe off the excess water on the surface with gauze, and test the compression performance of the material with a dynamic thermomechanical analyzer. Set the compression The speed is 1mm / min. Such as figure 2 As shown, after polydopamine coating, the mechanical properties of the stent have been improved to a certain extent, which may be because polydopamine enters the interior of the material system, and polydopamine itself has a certain adhesion effect, which makes the compressive modulus of the stent raised.

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Abstract

The invention relates to a preparation method of a hydrogel scaffold with a polydopamine coating, the preparation method comprises the following steps: dissolving anhydrous calcium chloride and a photoinitiator in deionized water, then adding modified gelatin and sodium alginate, and ultrasonically mixing uniformly to obtain mixed slurry; performing three-dimensional printing on the mixed slurry to obtain a pre-crosslinked composite scaffold; crosslinking the pre-crosslinked composite scaffold under ultraviolet light, and then soaking the pre-crosslinked composite scaffold in an anhydrous calcium chloride solution for secondary crosslinking to obtain a crosslinked composite scaffold; placing the cross-linked composite scaffold into a dopamine solution, taking out the cross-linked composite scaffold after reaction, washing the cross-linked composite scaffold with deionized water, and performing ultrasonic treatment to obtain the modified gelatin / sodium alginate composite scaffold with the polydopamine coating. By means of the method, the adhesion and osteogenic differentiation capacity of stem cells on the composite scaffold is improved, proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells are promoted, and the application range of the three-dimensional printing scaffold in the field of osteochondral cartilage is widened. The invention also relates to the hydrogel scaffold with the polydopamine coating.

Description

technical field [0001] The invention relates to the technical field of osteochondral repair, in particular to a hydrogel scaffold with a polydopamine coating and a preparation method thereof. Background technique [0002] The preparation of porous scaffolds by 3D printing technology has been widely used in the field of biomedicine. However, in the field of osteochondral repair, when using three-dimensional scaffolds to repair osteochondral, it will face the problem of insufficient number of cells. When cells are cultured in vitro, the number of planted cells is small, and it is difficult for cells to adhere to the scaffold. First, this is due to the characteristics of the three-dimensional scaffold itself. Because the scaffold itself has a large through-hole structure, cells tend to It will fall from the pores and finally fall on the top of the orifice plate, and there are fewer cells that actually grow on the scaffold; the second is because the material lacks biological ac...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/22A61L27/20A61L27/34A61L27/38B33Y10/00B33Y70/00B33Y80/00A61F2/28
CPCA61L27/52A61L27/222A61L27/20A61L27/34A61L27/3834A61L27/3852A61L27/3847B33Y10/00B33Y70/00B33Y80/00A61F2/28A61L2430/06A61L2430/02A61F2002/3006C08L5/04C08L79/04
Inventor 方立明陈文祥潘西满苏健裕祁海生
Owner SOUTH CHINA UNIV OF TECH