Isomaltooligosacharide prepared from ginkgo starch as well as preparation method and application thereof

A technology of isomaltooligosaccharide and starch, which is applied in the fields of application, function of food ingredients, food science, etc., can solve the problems of large amount of enzymes, high cost, and unseen problems, so as to simplify the production steps, reduce the types of use, and reduce the Effects of insufficient food resources

Pending Publication Date: 2021-07-20
NANJING FORESTRY UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no domestic research on the production of isomaltose oligosaccharides using ginkgo starch as a raw material using an all-enzyme method, and in the production of isomaltose reported in the literature, most of the saccharification steps use the method of compound enzyme ratio, and the amylases involved in the synergy include fungal α-amylase, pullulanase, β-amylase, and glucoamylase are produced in a variety of enzyme ratios of not less than three enzymes, which requires a large amount of enzymes and high costs
No operation has been seen for the saccharification step using only the two enzymes pullulanase and β-amylase

Method used

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  • Isomaltooligosacharide prepared from ginkgo starch as well as preparation method and application thereof
  • Isomaltooligosacharide prepared from ginkgo starch as well as preparation method and application thereof
  • Isomaltooligosacharide prepared from ginkgo starch as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] A method for preparing isomaltooligosaccharide with ginkgo starch, comprising the steps of:

[0045] (1) Preparation of ginkgo starch: shell the ginkgo seeds, remove the endocarp, mash, add 1% NaOH aqueous solution according to the mass ratio of 3:1, soak for 2 hours and centrifuge to get the precipitate, repeat the above method 3 times Combine the precipitates and wash with water until neutral to obtain wet starch, dry the wet starch by freeze-drying, seal it, and store it at room temperature;

[0046] (2) pulping: take by weighing 20g with the ginkgo starch that step (1) makes, join in the PBS damping fluid of 100mL, pH=6.0, be mixed with the ginkgo starch suspension that concentration is 20%, adjust pH to 5.8, Adjust the temperature of the water bath to 95°C, put it into a stirrer and stir for 5 minutes to gelatinize, and obtain a gelatinized liquid;

[0047] (3) Liquefaction: Add high-temperature-resistant α-amylase to the gelatinized liquid prepared in step (2) ac...

Embodiment 2

[0054] A method for preparing isomaltooligosaccharide with ginkgo starch, comprising the steps of:

[0055] (1) Preparation of ginkgo starch: shell the ginkgo seeds, remove the endocarp, mash, add 1% NaOH aqueous solution according to the mass ratio of 3.5:1, soak for 2.5h and then centrifuge to get the precipitate; repeat the above method for 4 times; combine the precipitates and wash to neutral to obtain wet starch, use freeze drying to dry the wet starch, seal it, and store it at room temperature;

[0056] (2) pulping: take by weighing the 25g ginkgo starch that step (1) makes, join in the PBS damping fluid of 100mL, pH=6.0, be mixed with the ginkgo starch suspension that concentration is 25%, adjust pH to 6.0, Adjust the temperature of the water bath to 98°C, put it into a stirrer and stir for 4 minutes to gelatinize to obtain a gelatinized liquid;

[0057] (3) Liquefaction: Add high-temperature-resistant α-amylase to the gelatinized liquid prepared in step (2) according ...

Embodiment 3

[0064] A method for preparing isomaltooligosaccharide with ginkgo starch, comprising the steps of:

[0065] (1) Preparation of ginkgo starch: shell the ginkgo seeds, remove the endocarp, mash, add 1% NaOH aqueous solution according to the mass ratio of 4:1, soak for 3 hours and then centrifuge to get the precipitate; repeat the above method 5 times Combine the precipitates and wash with water until neutral to obtain wet starch, dry the wet starch by freeze-drying, seal it, and store it at room temperature;

[0066] (2) Slurry: Take by weighing 30g of ginkgo starch prepared in step (1), add 100mL, PBS buffer solution of pH=6.0, be mixed with the ginkgo starch suspension that concentration is 30%, adjust pH to 6.2, adjust water bath The temperature of the pot is 100°C, put it into a stirrer and stir for 3 minutes to gelatinize, and obtain a gelatinized liquid;

[0067] (3) Liquefaction: Add high-temperature-resistant α-amylase to the gelatinized liquid prepared in step (2) acco...

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Abstract

The invention relates to the technical field of biological products, in particular to isomaltooligosacharide prepared from gingko starch and a preparation method and application thereof. The method for preparing the isomaltooligosacharide from the gingko starch comprises the steps of gingko starch extraction, pulp mixing, liquefaction, saccharification, glucoside conversion, fermentation purification and vacuum freeze drying aftertreatment to obtain the isomaltooligosacharide. According to the invention, the ginkgo starch is used as a raw material to produce the isomaltooligosacharide, the sum of four functional sugars of purified isomaltose, panose, isomaltotriose and isomaltotetraose is up to 54%, and by comparing the value-added effect of the ginkgo starch and commercially available corn isomaltooligosacharide on lactobacillus plantarum, the beneficial activity of the prepared isomaltooligosacharide exceeds that of common isomaltooligosacharide on the market at present. Aiming at the defects in the research field of producing isomaltooligosacharide from gingko starch, on one hand, the method for preparing isomaltooligosacharide from gingko starch is provided, and on the other hand, the utilization rate and economic value of gingko starch are improved, and the pressure of insufficient grain resources is relieved.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to isomaltooligosaccharide prepared from ginkgo starch and its preparation method and application. Background technique [0002] Ginkgo is a unique multi-purpose economic tree species in my country. The cultivation area of ​​Ginkgo in my country has reached 3 million mu, and the annual output of ginkgo has reached more than 30 million kilograms, and the dry green leaves are 60 million kilograms. At present, the ginkgo industry chain can be divided into three parts: nursery stock cultivation, landscaping and product processing, among which product processing plays a leading role in the ginkgo production chain. In the processing of ginkgo products, ginkgo fruit is rich in nutrition, contains flavonoids, lactones and other biologically active components, has a very high medical and health value, and has a history of more than 1,000 years as a nourishing and health food. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/22C12P19/18C12P19/16C12P19/14C12P19/00C12P19/04A23L33/21
CPCC12P19/22C12P19/18C12P19/16C12P19/14C12P19/00C12P19/04A23L33/21A23V2002/00A23V2250/6412A23V2200/3202
Inventor 庄国宏李婷婷宋阳董莉莉张心怡吴彩娥范龚健陈明蔡雯瑞
Owner NANJING FORESTRY UNIV
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