A non-damaging circulation culture device for salina

Abstract

The invention provides a non-damaging circulating culture device for salina, comprising a liquid level difference airlift reactor using a combination of negative pressure and positive pressure, a photobioreactor communicating with the upper end and the lower end of the airlift reactor, The air-lift reactor adds a bypass cycle, which can independently control the size, size, or opening and closing of the valve according to the dissolved oxygen concentration in the device. In addition, the carbon dioxide gas supply device is structurally separated from the dissolved oxygen analysis mechanism. The invention adopts a jet to introduce sterilized compressed air to lift the salt algae liquid up to realize a non-damaging circulation, the carbon dioxide replenishment module and the dissolved oxygen analysis module are physically separated, and the bypass circulation can adjust the bypass circulation flow rate and circulation time according to the dissolved oxygen value in the exchange column , improve the utilization efficiency of carbon dioxide, and then promote the photosynthesis of salina cells to increase the biological yield, and finally realize the artificial controllability of salina culture in brine.

Description

technical field [0001] The invention relates to the technical field of salina cultivation devices, in particular to a salina non-damaging circulation cultivation device. Background technique [0002] Salina is a single-celled green alga with double flagella. The length of the flagella is 1-2 times the length of the cell, and it is extremely vulnerable to external mechanical damage. Its cell wall structure is mainly composed of glycoprotein complexes and lacks rigid cellulose components. This flexible cell structure also makes Salina cells extremely vulnerable to external mechanical shear damage and growth in the process of industrial cultivation. decline and even cell death. In addition, the damage and death of salina cells will release a large amount of organic matter into the culture system, which will promote the reproduction of bacteria and even harmful organisms; the organic matter such as protein dissolved in the system will generate a large number of air bubbles unde...

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Problems solved by technology

Cai Zhiwu's "A production device for industrialized cultivation of microalgae" (Chinese patent, CN201245640Y) discloses a closed photobioreactor model operated by air push, but this invention is only applicable to liquids flowing horizontally

"A kind of salina culture device" (Chinese patent, CN209722114U) of Luo Guanghong et al. uses a motor to drive the stirring shaft and stirring fins to stir the salina culture solution to avoid cell attachment, but its mechanical rotation will still introduce exogenous machinery. Shear force, which may damage the cells; in addition, agitation will increase the energy consumption of the equipment

"A self-absorbing oxygen-type tubular photobioreactor" (Chinese patent, CN107043693B) by He Yaling et al. absorbs the high concentration of dissolved oxygen in the reactor by introducing the fuel cell stack, but still needs the aid of a circulating water pump to realize the algae liquid. cycle

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