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Application of Neofomitella fumosipora Han in laccase production and laccase production method from Neofomitella fumosipora Han

The technology of layer-porous bacteria and gray-porous bacteria is applied in the application of new microporous layer-porous bacteria in the field of laccase production and its induction and acceleration of laccase production, which can solve the problem of complex fermentation substrate components, shortening laccase production cycle, and lacquer production. The problem of high cost of enzyme production, to achieve the effect of convenient purification and utilization, shortening the production cycle, and conducive to environmental protection

Pending Publication Date: 2021-07-23
LANGFANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of ash pore new microporia in order to solve the technical problems such as low laccase yield, long enzyme time, complicated fermentation matrix components and high laccase production cost in the existing process of using fungi to produce laccase. The application in the production of laccase and the method for producing laccase, the method of the present invention inoculates the fungus Neofominella fumosipora Han 386 into the basal medium containing lignocellulosic materials, carries out fermentation and culture, and obtains Utilize the method of producing laccase efficiently and quickly by the bacteria Neolamina spp., the method of the present invention has short fermentation time, high activity of laccase, and significant extraction of laccase production time, greatly shortens the production cycle of laccase and improves the production efficiency of laccase , reduce production cost of laccase

Method used

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  • Application of Neofomitella fumosipora Han in laccase production and laccase production method from Neofomitella fumosipora Han

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Experimental program
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Effect test

Embodiment 1

[0050] 1. Experimental strains

[0051] Neofomitella fumosipora Han 386 was provided by the North China Forestry Experimental Center of the Chinese Academy of Forestry and is now preserved in the College of Life Sciences, Langfang Teachers College.

[0052] The above-mentioned bacterial strain of the present invention is provided by the North China Forestry Experimental Center of the Chinese Academy of Forestry Sciences, and its strain number is HFNC 851 in the North China Forestry Experimental Center Herbarium of the Chinese Academy of Forestry Sciences, and its preservation address is No. 1, Shuizha West Road, Mentougou District, Beijing.

[0053] 2. Medium

[0054] Malt extract agar medium (Malt extract agar, MEA): glucose 10g, malt extract powder 20g, potassium dihydrogen phosphate 3g, agar 20g, water to 1L.

[0055] Complete Yeast Medium (CYM): glucose 20g, peptone 2g, yeast extract powder 2g, magnesium sulfate heptahydrate 0.5g, dipotassium hydrogen phosphate trihydrate...

Embodiment 2

[0064] 2-1), N.fumosipora Han 386 provided by the North China Forestry Experimental Center of the Chinese Academy of Forestry and stored on the malt extract powder agar medium MEA was inoculated into solid complete medium (CYM plate Complete culture medium), under the condition of 26±0.5°C (usually 25-28°C), constant temperature culture for 8 days (usually 7-9 days), and strain activation treatment;

[0065] 2-2), use a sterile puncher to punch the activated culture of the strain after 8 days of activation culture, and obtain an expanded culture inoculum with a diameter of 5 mm; then, under aseptic conditions, inoculate the expanded culture inoculum to In the liquid complete medium, wherein in every 100mL liquid complete medium, inoculate 5 described expansion culture inoculums; Then carry out dark liquid expansion culture on the shaker, obtain the expansion culture mixture (containing expansion Cultivate to obtain gray hole new small layer hole mycelium ball); wherein the cul...

Embodiment 3

[0079] In addition to the corresponding Beijing poplar wood powder, Chinese toon wood powder, and weeping willow wood powder in medium 1 (T1), medium 2 (T2), medium 3 (T3) and medium 4 (T4) in steps 2-4) , The consumption of sorghum stalk powder is respectively except 2g, and all the other are identical with embodiment 2.

[0080] The laccase activity measurement result shows: Neofomitella fumosipora Han 386 of the present invention uses the culture medium of the present invention as the culture medium to induce culture, all just can reach maximum production laccase activity on the 1st day, and T2 culture medium The activity of producing laccase in the culture medium containing Toona sinensis wood flour was higher than that in culture medium T1, T3 and T4.

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Abstract

The invention discloses an application of Neofomitella fumosipora Han in laccase production and a laccase production method from the Neofomitella fumosipora Han. The Neofomitella fumosipora Han 386 is inoculated into a basal culture medium containing a lignocellulose material, fermentation culture is carried out, and the laccase is rapidly produced. According to the method disclosed by the invention, high laccase activity can be obtained in the first day of fermentation by utilizing the Neofomitella fumosipora Han, so that the whole laccase fermentation production time is quickly advanced, the laccase production period is remarkably shortened, the laccase yield and the laccase production efficiency are improved, and the laccase production cost is reduced; in addition, the components of the fermentation culture medium used in the method are simple and easy to obtain; the fermentation method is simple, operation conditions are easy to control, and product quality is stable and reliable; and time and labor are saved, repetition is easy, and wide application and popularization are facilitated.

Description

technical field [0001] The present invention relates to a new application of microorganisms and a method for accelerating the production of laccase, in particular to the application of a strain of Neophyropora cinereus in producing laccase and its method for inducing and accelerating the production of laccase, which belongs to fungi application field. Background technique [0002] The application of enzymes is a biocatalyst process with a fast conversion rate in biochemical reactions. Therefore, enzymes generally play an important role in various aspects of biotechnology and industry. Compared with pure chemical methods, enzymatic methods have the advantages of fast catalytic efficiency, friendly control, high substrate specificity, wide substrate range, energy saving, mild reaction process, and non-toxicity. Enzymes are usually proteins with the ability to degrade a wide variety of complex substances into other intermediate metabolites. Therefore, enzymes are beneficial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12R1/645
CPCC12N9/0061C12Y110/03002
Inventor 安琪韩美玲侯晓强付亚娟闫训友吴智艳
Owner LANGFANG NORMAL UNIV
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