Whole blood DNA extraction kit and extraction method

Abstract

The invention belongs to the technical field of kits, and particularly relates to a whole blood DNA extraction kit. The whole blood DNA extraction kit comprises a buffer solution, a lysis solution, protease K, a purification solution, a washing solution 1, a washing solution 2 and an eluent; the buffer solution consists of 10 mM of Tris, 10 mM of NaCl and 5 mM of MgCl2; the lysis solution consists of 5 mM of guanidine isothiocyanate, 5 M of lithium iodide, 200 mM of trisodium citrate and 5% of Tween20; the protease K consists of of 20 mg of protease K; the purification solution consists of 7.5 M of lithium chloride and 7.5 M of sodium chloride; and the washing solution 1 consists of 4 mM of guanidine isothiocyanate, 50 mM of Tris-HCL and 250% of absolute ethyl alcohol. The whole blood DNA extraction kit has the beneficial effects that a used reagent does not contain toxic substances such as benzene, chloroform and the like and is harmless to a human body, extraction and purification steps are simple, the operation process is safe and convenient, the genome DNA product has high content, good integrity and high purity, the method is convenient for a worker to operate, the labor intensity of the worker is reduced, and subsequent experiments of researchers are facilitated.

Description

technical field [0001] The invention belongs to the technical field of kits, and in particular relates to a whole blood DNA extraction kit and an extraction method. Background technique [0002] With the development of molecular biology techniques, research at the genome level has become a hot spot. In molecular genetics and molecular epidemiology, whether it is genome-wide association analysis (GWAS), genotyping of candidate SNP sites, or genomic library The establishment of all kinds of samples needs to extract genomic DNA from various samples. Since blood samples are convenient to obtain and contain abundant and reliable genomic DNA, many of the above studies use blood as a sample to extract complete genomic DNA. [0003] Although the traditional method of extracting nucleic acid with phenol chloroform can obtain better quality nucleic acid, the chemical substances used in it have non-negligible toxicity, and the whole extraction process takes a long time, wasting a lot o...

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