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Method for preparing Anti-helicobacter pylori egg yolk antibody

A technology against Helicobacter pylori and Helicobacter pylori, which is applied in the direction of antibodies, antibacterial drugs, chemical instruments and methods, etc., to achieve the effect of high specificity and binding, and growth inhibition

Pending Publication Date: 2021-07-23
(株)AD生物技术
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] As a method for treating diseases caused by the infection of the above-mentioned Helicobacter pylori, chemotherapy utilizing antibiotics is given priority, however, there are side effects such as the emergence of antibiotic-resistant strains caused by continuous use of antibiotics, which is not preferable, so , is a very urgent reality for the development of new vaccines for the prevention and treatment of infection by Helicobacter pylori

Method used

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  • Method for preparing Anti-helicobacter pylori egg yolk antibody
  • Method for preparing Anti-helicobacter pylori egg yolk antibody
  • Method for preparing Anti-helicobacter pylori egg yolk antibody

Examples

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Effect test

preparation example 1

[0038] Preparation Example 1: Production of Antigen

[0039] 1) Helicobacter pylori (H. pylori)

[0040] As Helicobacter pylori, 43504 strain purchased from ATCC was used. Bacteria were scraped off (scraping) into blood agar (blood agar) with a sterilized cotton swab and placed in a bag equipped with a gas-pak, and placed in a 37°C incubator in CO 2 Cultured for 3 days under the condition of 10%. After culturing, bacteria were recovered using sterilized cotton swabs, and cultured at 37° C. in CO using a culture medium supplemented with 5% horse serum and antibiotics in Brucella broth (Difco Laboratories, USA). 2 Shaking culture was carried out under the condition of 10%. Thereafter, the antigen was inactivated by treatment with 0.1% formalin, and left standing at room temperature for 2 days. Then, after centrifugation at 6000 rpm for 20 minutes, the pellet was suspended in 1×PBS, treated with 0.1% formalin again, and stored in a refrigerator for use as an antigen.

preparation example 2

[0041] Preparation Example 2: Preparation of Mouse Antibody

[0042] Toxicity was suppressed by inactivating the antigen produced in Preparation Example 1 above, and it was stored in a refrigerator before use. A mouse antibody preparation vaccine was prepared using a complete adjuvant. Antigen vaccines were prepared and antibodies were prepared according to conventional mouse antibody preparation methods. Mouse antibodies were purified using a protein-A column from the recovered serum (Serum) after production.

preparation example 3

[0043] Preparation Example 3: Preparation of Antigen-Heterologous Antibody Complex

[0044] In order to bind the antigen produced in Preparation Example 1 above with the mouse antibody produced in Preparation Example 2, the following experiments were carried out. 100 μg of mouse antibodies produced against each antigen were mixed with the inactivated mixed vaccine, and antigen-antibody binding was induced at 4° C. overnight. The combined sample was centrifuged and recovered, and used as a sample for inoculation of laying hens.

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Abstract

The present invention relates to a method for preparing an anti-Helicobacter pylori egg yolk antibody, comprising injecting a Helicobacter pylori antigen into an animal that is different from a chicken so as to prepare a heterologous antibody, and then injecting, into a laying hen, a complex in which the antigen and heterologous antibody are combined, thereby preparing an egg yolk antibody. According to the present invention, the method for preparing an anti-Helicobacter pylori egg yolk antibody, of the present invention, can mass-manufacture and -produce an egg yolk antibody through the injection of a heterologous antigen-antibody complex into a laying hen, and the egg yolk antibody prepared thereby has high specificity and avidity to Helicobacter pylori, thereby effectively inhibiting the bacteria.

Description

technical field [0001] The present invention relates to the preparation method of anti-Helicobacter pylori egg yolk antibody, more specifically, to the preparation method of anti-Helicobacter pylori egg yolk antibody, that is, preparing heterologous After inoculating the complex of the above-mentioned antigen and heterologous antibody into laying hens, the egg yolk antibody is prepared. Background technique [0002] Antibody is a globulin protein formed by B lymphocytes or B cells in lymphoid tissue, and it is one of the important substances that interfere with specific immune recognition. Antibodies have two different functions. The first function is to neutralize the effect of antigens by specifically recognizing and binding to specific parts of antigens that generate immune responses. The second function is to collect antibodies by combining antibodies with antigens. Various substances and immune cell functions for removing antigens. For example, antibodies can neutrali...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K16/02A61K39/40A61P31/04A61P1/04A61P35/00
CPCC07K16/121C07K16/02A61P31/04A61P1/04A61P35/00C07K2317/11A61K2039/505
Inventor 郑洪杰白斗渊元美庆裴海得
Owner (株)AD生物技术
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