Aggregation-induced emission peptide assembly, preparation method, detection method and application of aggregation-induced emission peptide assembly
A technology of aggregation-induced luminescence and aggregation-induced fluorescence, which is applied in the field of biomedical engineering materials, can solve the problems of inability to meet the detection requirements of bacterial endotoxin, insufficient stability and sensitivity of the sensor, etc., achieve good market transformation prospects, difficult test results, and easy preparation Effect
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Embodiment 1
[0056] This embodiment provides a polydopamine-coated black phosphorus nanosheet, the specific steps of preparation are:
[0057] A1 Put lumpy black phosphorus in 20ml of anhydrous 1-methyl-2-pyrrolidone, sonicate for 0.5h to fully disperse, place in an ice-water bath to maintain the temperature at 15°C, and use a 650w ultrasonic breaker to ultrasonically break for 6h , set the single ultrasonic time to 10s, and the single interval time to 5s, then centrifuge at 2000rpm for 20min to remove unexfoliated black phosphorus particles, collect the brown supernatant, and store it at low temperature;
[0058] A2 Centrifuge the brown supernatant obtained in step A1 at a speed of 9000rpm for 15min to remove 1-methyl-2-pyrrolidone, collect the flaky black phosphorus precipitate after peeling off, and freeze-dry the flaky black phosphorus precipitate. Ultrasonic dispersion for 0.5-1h to uniformly disperse in 5ml of absolute ethanol, add 10μL of dopamine hydrochloride aqueous solution and ...
Embodiment 2
[0062] This embodiment provides a polydopamine-coated black phosphorus nanosheet, the specific steps of preparation are:
[0063] A1 Put block black phosphorus in 20ml of anhydrous 1-methyl-2-pyrrolidone, sonicate for 1h to fully disperse, place in an ice-water bath to maintain the temperature at 25°C and use a 650w ultrasonic breaker for 9h, Set the single ultrasonic time to 10s, the single interval time to 5s, then centrifuge at 4000rpm for 30min to remove unexfoliated black phosphorus particles, collect the brown supernatant, and store it at low temperature;
[0064] A2 Centrifuge the brown supernatant obtained in step A1 at a speed of 12000rpm for 30min to remove 1-methyl-2-pyrrolidone, collect the flaky black phosphorus precipitate after peeling off, and freeze-dry the flaky black phosphorus precipitate. Ultrasonic dispersion for 1 h to uniformly disperse in 10 ml of absolute ethanol, add 50 μL of dopamine hydrochloride aqueous solution and 50 μL of sodium hydroxide aqueo...
Embodiment 3
[0068] This example provides a bacterial endotoxin targeting peptide modified by aggregation-induced fluorescent molecules, and the specific steps of preparation are as follows:
[0069] Dissolve a certain amount of tetraphenylethylene derivatives at room temperature in 5-10ml PBS phosphate buffer solution, add a certain amount of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, stirred for 3-5 hours, added bacterial endotoxin targeting peptide to react for 10-15 hours, protected from light, dialyzed in a dialysis bag, and freeze-dried to obtain tetraphenylethylene-modified bacterial endotoxin targeting peptide.
[0070] The mol ratio of described tetraphenylethylene derivative, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and bacterial endotoxin targeting peptide is 1:2:2:2; the molecular weight intercepted by the dialysis bag is 1000-2000Da.
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Abstract
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