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Method for detecting biomolecules by using Lamb wave resonator and microfluidic device

A microfluidic and resonator technology, applied in the field of detection of biological substances, can solve the problems of poor specificity, high voltage, easy blocking and so on

Pending Publication Date: 2021-08-03
SAVELIFE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, these technologies are limited by the disadvantages of high voltage (hundreds to thousands of volts), low flux, and easy blockage. They need to generate a very large field intensity distribution locally, which has great limitations on molecular manipulation.
At the same time, manipulating molecules directly using physics is less specific

Method used

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  • Method for detecting biomolecules by using Lamb wave resonator and microfluidic device
  • Method for detecting biomolecules by using Lamb wave resonator and microfluidic device
  • Method for detecting biomolecules by using Lamb wave resonator and microfluidic device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Preparation of microfluidic channel and Lamb wave resonator (Lamb Wave Resonator, LWR):

[0084] Manufacturing process of Lamb wave resonator based on CMOS compatible process

[0085] figure 1 Schematic diagram of the manufacturing process. First, the silicon substrate is etched by reactive ion etching, and an air cavity ( figure 1 a). Afterwards, phosphosilicate glass (PSG) used as a sacrificial layer was deposited on the substrate using chemical vapor deposition (CVD) ( figure 1 b). After planarization by chemical mechanical polishing (CMP) ( figure 1 c), followed by step-by-step deposition of the bottom electrode (200nm Mo film) on the substrate ( figure 1 d), the piezoelectric layer (450nm AlN film) ( figure 1 e) and the top electrode (200nm Mo film) ( figure 1 f). Then, based on Cl 2 plasma etching and potassium hydroxide wet etching to etch the AlN film ( figure 1 g). Afterwards, Au was deposited by evaporation and partially removed by exfoliation to f...

Embodiment 2

[0091] In the specific implementation process of this embodiment, a microfluidic device is provided, which can be used to detect the particle-target biological substance complex in the sample.

[0092] The method of the present invention involves adopting a microfluidic device with a Lamb wave resonator, converging the particles combined with the target biological substance into the swirling flow generated by the bulk acoustic wave emitted by the Lamb wave resonator in the fluid, realizing the control of the Enrichment and detection of biological substances and detectable labels thereon. figure 2It is a schematic diagram of an exemplary application of the method and microfluidic device of the present invention. The figure on the left shows: a Lamb wave bulk acoustic resonator is set at the bottom of the microchannel of the microfluidic device (top not shown); PS microspheres and PSA functionalized with capture antibodies are introduced from the entrance of the microchannel. ...

Embodiment 3

[0105] Materials and Experiments

[0106] The method of the present invention involves the specific binding of particle-ligand-target substance. In the exemplary embodiment of the present application, one of the target substances is prostate specific antigen (PSA), and its ligand is an anti-PSA antibody. PSA or its antibodies were labeled with different fluorescent signals, respectively.

[0107] In the examples of this application, commercially available polystyrene (PS) microspheres were used as particles. Label PS microspheres with fluorophores or protein moieties.

[0108] Relevant materials include:

[0109] FITC-labeled polystyrene particles were purchased from Shanghai Aladdin Industrial Corporation (Shanghai, China);

[0110] Prostate-specific antigen (PSA), capture antibody (mouse anti-PSA antibody, primary antibody, Ab1) and detection antibody (horseradish peroxidase-modified anti-PSA antibody, secondary antibody, Ab2) were purchased from Linc-bio (Shanghai) , w...

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Abstract

The invention provides a method for enriching and detecting small biological substances by using a microfluidic system for generating bulk acoustic waves by using a Lamb wave resonator. The invention also provides equipment or a device for enriching and detecting the small biological substances based on the method. The method and equipment provided by the invention have the advantages of high sensitivity, short detection time and simplicity and convenience in operation, and are suitable for miniaturized instant detection.

Description

[0001] This application claims the priority of the following Chinese patent application: filed on February 3, 2020, the application number is 202010078266.3, and the title of the invention is "Method and microfluidic device for detecting biomolecules using a Lamb wave resonator", the entire content of which Incorporated in this application by reference. technical field [0002] The invention relates to the fields of cell research methodology and medical equipment. Specifically, the present invention relates to a microfluidic device for detecting biomolecules using a Lamb wave resonator and a method for using the device to detect biological substances. Background technique [0003] In modern biomedical engineering, the manipulation and control of micron-scale cells or even smaller submicron-scale biochemical macromolecules is the key to realizing various biomedical engineering, such as cell imaging, biomolecular enrichment, artificial tissue generation, etc. important means....

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N15/00
CPCB01L3/502761B01L3/50273G01N15/00B01L2200/0647B01L2300/0861B01L2400/0436G01N15/01
Inventor 段学欣陈娴
Owner SAVELIFE BIOTECHNOLOGY CO LTD
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