Method for simultaneously detecting gamma-lactone and delta-lactone compounds in cheddar cheese
A technology of cheddar cheese and compounds, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of research and testing standards, and achieve the effect of strong selectivity and good repeatability
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Embodiment 1
[0033] Example 1: Determination of Eleven Kinds of Lactone Compounds GC-MS Detection Method
[0034] (1) Preparation of internal standard solution: Accurately weigh 0.22g of 2-octanol in a 100mL volumetric flask, dissolve it with ultrapure water and adjust the volume to the mark. The internal standard solution is stored at 4°C, and the validity period is 3 months;
[0035] (2) Preparation of 11 kinds of γ-lactone and δ-lactone mixed standard solution a: Weigh 0.1g (accurate to 0.1mg) of 11 kinds of γ-lactone and δ-lactone standards respectively, and dichloromethane As a solvent, dilute to a 100mL volumetric flask, and prepare a mixed standard solution a with a concentration of 1mg / mL. The mixed standard solution a is stored at 4°C and is valid for 3 months.
[0036] (3) Preparation of 11 kinds of γ-lactone and δ-lactone mixed standard solutions b: Accurately pipette 5mL mixed standard solution a, put it in a 50mL volumetric flask, dilute to the mark with dichloromethane, and...
Embodiment 2
[0043] Embodiment 2: Determination of the standard working curve of eleven kinds of lactone compounds
[0044] Freshly made cheddar cheese was selected as the blank matrix, and standard working solutions of different concentrations and 20 μL of 2-octanol (concentration of 220 mg / L) were added to the blank matrix, and the mixed matrix standard working solution was obtained after SAFE treatment for analysis. The chromatographic peak area of 11 kinds of γ-lactone and δ-lactone compounds and the internal standard 2-octanol in the matrix standard working solution is the ordinate (Y), and the corresponding mass concentration is the abscissa (X) to draw the standard curve , and carry out regression analysis, the regression equation and correlation coefficient are shown in Table 2.
[0045] Standard working curve and correlation coefficient of each lactone compound in table 2
[0046] lactone compound Standard working curve correlation coefficient γ-butyrolactone ...
Embodiment 3
[0047] Embodiment 3: the determination of lactone compound in the cheese to be tested
[0048] Weigh 30g of shredded cheddar cheese sample into a 250mL Erlenmeyer flask, add 60mL of dichloromethane, tie the mouth of the bottle tightly with gauze and cotton rope, shake it in a shaker at 4°C and 150r / min for 3h to obtain the extract; The obtained extract was centrifuged at 4° C. and 6000 rpm for 5 min, and the two phases were separated through a separatory funnel to obtain a clear sample liquid. For the obtained clarified sample liquid, the degree of vacuum is 10 -5 Torr, solvent-assisted flavor evaporation (SAFE) was performed while the circulating water bath was kept at a constant temperature of 50°C. After the treatment was completed, the liquid in the collection bottle was concentrated by nitrogen blowing, the temperature of nitrogen blowing was set at 15°C, and the pressure of nitrogen blowing was maintained at 5Pa. After nitrogen blowing, the concentrate was taken for GC...
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