Use of fluridone as a strigolactone inhibitor

The technology of fluridone and the fluridone, which is applied to the application field of fluridone as a strigolactone inhibitor, can solve the problems of high price, lack of a growth regulator of strigolactone inhibitor, and the like, and achieves the Inexpensive, enhances resistance to stress, and promotes plant branching

Active Publication Date: 2022-06-21
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price, there is no growth regulator of strigolactone inhibitors applicable to agricultural production at present.

Method used

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  • Use of fluridone as a strigolactone inhibitor
  • Use of fluridone as a strigolactone inhibitor
  • Use of fluridone as a strigolactone inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The effect of the spray concentration of fluridone solution on the branching of Arabidopsis thaliana

[0024] The experiment was repeated three times, and the method for each repetition was as follows:

[0025] (1) Material preparation. Arabidopsis was used as a model plant.

[0026] (2) Seed selection and disinfection. The Arabidopsis thaliana seeds were sequentially carried out according to the following steps: selecting the Columbia wild-type Arabidopsis thaliana seeds, soaking and disinfecting the seeds with 75% ethanol for 15 minutes; washing with sterilized deionized water for 5 times to obtain the Arabidopsis thaliana sterilized seeds.

[0027] (3) Seedling growth. The Arabidopsis thaliana seeds obtained in step (2) were sown on 1 / 2 MS solid medium, placed in a 4°C refrigerator for vernalization for 3 days, and then placed at 22°C for 7 days under 16h light and 8h dark conditions. When Arabidopsis grew 4 leaves, it was transplanted into small pots (...

Embodiment 2

[0034] Example 2 The effect of fluridone spray concentration on the branching of Arabidopsis thaliana

[0035] The experimental steps of the effect of fluoridone spray concentration on the branches of Arabidopsis are as follows:

[0036] (1) Material preparation. Arabidopsis was used as a model plant.

[0037] (2) Seed selection and disinfection. The Arabidopsis thaliana seeds are sequentially carried out according to the following steps: select the Columbia wild-type Arabidopsis thaliana seeds, use 75% ethanol to soak and disinfect the seeds for 15 minutes; wash with sterilized deionized water for 3-5 times to obtain the Arabidopsis thaliana disinfection seed.

[0038] (3) Seedling growth. The Arabidopsis thaliana seeds obtained in step (2) were sown on 1 / 2 MS solid medium, placed in a 4°C refrigerator for vernalization for 3 days, and then placed at 22°C for 7 days under 16h light and 8h dark conditions. When Arabidopsis grew 4 leaves, it was transplanted into small pot...

Embodiment 3

[0044] Example 3 The effect of fluridone spray concentration on the growth of Arabidopsis

[0045] The Arabidopsis thaliana plants sprayed with different concentrations of fluridone reagent ((0.01, 0.05, 0.1, 0.5, 1 μM) and control group CK) in Example 1 for one week were tested to determine their photosynthetic intensity under different concentration treatments. and physiological indicators.

[0046]Photosynthetic intensity measurement: use LI-COR / LI-6400 portable photosynthesis measurement system to measure and output photosynthetic intensity index.

[0047] The effects of different concentrations of fluridone solution on the leaves of Arabidopsis thaliana are as follows: figure 2 As shown in the figure, it can be seen from the figure that the leaves of Arabidopsis thaliana in the 0.1-1 μM concentration of fluridone solution all turn yellow to a certain extent. The photosynthetic intensity was measured as image 3 As shown in the figure, it can be seen from the figure th...

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Abstract

The invention discloses the application of fluridone as a strigolactone inhibitor. The fluridone can be used as a plant strigolactone inhibitor to promote plant branching, has obvious effect on increasing production of plants, and can The method is applied to various plants, has little environmental pollution, and the fluridone reagent is cheap, and can be applied on a large scale in agricultural production.

Description

technical field [0001] The invention belongs to the technical field of pesticides, in particular to the application of fluridone as a strigolactone inhibitor. Background technique [0002] Strigolactones is a plant hormone or its precursor discovered in recent years, which can inhibit the growth of plant branching and lateral buds. It cooperates with auxin and cytokinin to control plant branching (tiller). quantity. As a carotenoid derivative produced in plant roots, strigolactone promotes symbiosis between plants and soil microorganisms. In addition, in the process of interaction between plants and their parasitic plants, strigolactone also acts as a signal molecule, which can induce parasitic plants such as Striga spp., Orobanche spp. seeds germination. [0003] Due to the functional properties of strigolactone, it has become a new means of regulating plant branching, directly or indirectly inhibiting the germination of plant lateral buds to produce branching, regulatin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/40A01P21/00A01G7/06A01G13/00
CPCA01N43/40A01G7/06A01G13/00Y02A40/10
Inventor 肖浪涛苏益寻敏
Owner HUNAN AGRICULTURAL UNIV
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