Bifidobacterium lactis MN-Gup dairy product and application thereof in improving type 2 diabetes mellitus

A technology for type 2 diabetes and Bifidobacterium lactis, applied in bifidobacteria, dairy products, applications, etc., can solve problems such as reducing intervention effects, adverse drug side effects, and easily causing drug resistance, reducing blood sugar levels and improving regulation. Intestinal flora balance, the effect of restoring healthy intestinal flora

Active Publication Date: 2021-08-06
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in addition to lifestyle interventions, common interventions for type 2 diabetes are mainly hypoglycemic drug interventions, such as hypoglycemic drugs metformin, α-glucosidase inhibitors, thiazolidinedione drugs (TZDs) and GLP-1 receptor Drug therapy such as agonists can reduce the risk of diabetes in pre-diabetic populations, but most of these drugs will have adverse side effects, and are likely to cause drug resistance, reducing the effect of intervention

Method used

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  • Bifidobacterium lactis MN-Gup dairy product and application thereof in improving type 2 diabetes mellitus
  • Bifidobacterium lactis MN-Gup dairy product and application thereof in improving type 2 diabetes mellitus
  • Bifidobacterium lactis MN-Gup dairy product and application thereof in improving type 2 diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] 1. Preparation of Bifidobacterium lactis MN-Gup bacterial powder

[0097] The fermented strain Bifidobacterium lactis MN-Gup (the strain is preserved in the General Microorganism Center of the China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No. 15578) is activated and cultured with MRS, and cultured at 37°C for 16-20h, and cultured To the logarithmic phase, the number of viable bacteria reached 10 9 cfu / mL.

[0098] Bifidobacterium lactis MN-Gup was centrifuged at 6000rpm for 2.5h to collect wet cells, and lactose was added as a freeze-drying protectant. The mass ratio of the amount of freeze-drying protectant to the collected cells was 0.8:1 to obtain a concentrated bacterial liquid , the concentrated bacterial solution was freeze-dried at -50°C for 24 hours to obtain Mn-Gup bacterial powder, and the number of viable bacteria was greater than 3×10 11 cfu / g.

[0099] 2. Preparation of Mn-Gup fermented milk with impr...

Embodiment 2

[0124] 1. Improvement effect of Bifidobacterium lactis MN-Gup fermented milk on T2DM mouse model

[0125] Select 60 male SPF grade C57BL / 6J male mice aged 6 weeks, and house all the mice in an animal room with a constant temperature of 21-25°C and a humidity of 40-70%, and the room maintains a 12-hour light / dark cycle. The breeding process complied with the relevant guidelines for the management and protection of experimental animals. Mice were first fed with common feed to adapt to the environment for 1 week (recorded as the first week of the experiment). Then they were randomly divided into 4 groups, 15 mice in each group, namely control group, model group, HFD+Mn-Gup-low dose group and HFD+Mn-Gup-high dose group.

[0126] Control group: fed with common feed during the 2nd to 13th weeks of the experiment;

[0127] Model group: fed with high-fat feed. After 5 weeks of high-fat diet induction, the mice in the high-fat diet (HFD) group were intraperitoneally injected with st...

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Abstract

The invention provides a dairy product capable of improving type 2 diabetes mellitus or regulating intestinal flora. The dairy product contains bifidobacterium lactis MN-Gup. The dairy product provided by the invention can improve the characteristic intestinal flora of the type 2 diabetes mellitus in a targeted manner, and regulate the diversity of the intestinal flora and/or the balance of the intestinal flora; the dairy product can improve the type 2 diabetes mellitus through a comprehensive mechanism, including significant relieving of weight loss and islet injury, blood sugar level reduction, intestinal mucosa repairing, intestinal insulin improvement, in-vivo inflammation state improvement and healthy intestinal flora recovery.

Description

technical field [0001] The invention relates to the field of food, in particular to the application of a bifidobacterium lactis MN-Gup dairy product in improving type 2 diabetes. Background technique [0002] Type 2 diabetes mellitus (T2DM) is a common metabolic disorder worldwide characterized by hyperglycemia due to insulin resistance and relative insulin deficiency. Metabolic abnormalities in T2DM may lead to multiple serious complications, such as cardiovascular disease, diabetic retinopathy, neuropathy, and nephropathy. Diabetes imposes a heavy burden on global health and is one of the leading causes of death worldwide. The gut microbiota plays an important role in the pathogenesis and metabolic disturbances of T2DM. [0003] The gut microbiota is a complex overall ecosystem, and the genome of the entire gut microbiota, known as the "microbiome," exceeds the human nuclear genome by at least 100-fold. Excessive intake of high-fat and fructose foods disturbs the normal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/123A23C9/13
CPCA23C9/123A23C9/13A23C9/1307A23C9/1322A23C9/1315A23V2400/531Y02A50/30
Inventor 陈建国韩雨婷孙二娜孙健康小红朱振华房洪涛史苏华
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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